Cellular and molecular responses of irradiated glioblastoma cell lines under inhib...
| Full text | |
| Author(s): |
Borges, Kleiton Silva
[1]
;
Brassesco, Maria Sol
[2]
;
Scrideli, Carlos Alberto
[2]
;
Egea Soares, Ademilson Espencer
[1]
;
Tone, Luiz Gonzaga
[1, 2]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14048900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pediat, Escola Med Ribeirao Preto, Div Pediat Oncol, BR-14048900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | GENETICS AND MOLECULAR BIOLOGY; v. 34, n. 2, p. 310-314, 2011. |
| Web of Science Citations: | 17 |
| Abstract | |
Propolis is a resin formed by a complex chemical composition of substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine, due to its biological properties, that include antimicrobial, anti-inflammatory, antitumoral and immunomodulatory activities. Glioblastoma is the most common human brain tumor. Despite the improvements in GBM standard treatment, patients' prognosis is still very poor. The aim of this work was to evaluate in vitro the Tubi-bee propolis effects on human glioblastoma (U251 and U343) and fibroblast (MRC-5) cell lines. Proliferation, clonogenic capacity and apoptosis were analyzed after treatment with 1 mg/mL and 2 mg/mL propolis concentrations for different time periods. Additionally, glioblastoma cell lines were submitted to treatment with propolis combined with temozolomide (TMZ). Data showed an antiproliferative effect of tubi-bee propolis against glioblastoma and fibroblast cell lines. Combination of propolis with TMZ had a synergic antiproliferative effect. Moreover, propolis caused decrease in colony formation in glioblastoma cell lines. Propolis treatment had no effects on apoptosis, demonstrating a cytostatic action. Further investigations are needed to elucidate the molecular mechanism of the antitumor effect of propolis, and the study of its individual components may reveal specific molecules with antiproliferative capacity. (AU) | |
| FAPESP's process: | 10/08699-5 - Mechanisms involved in the inhibition of aurora-kinases in adrenal carcinoma |
| Grantee: | Kleiton Silva Borges |
| Support type: | Scholarships in Brazil - Doctorate |