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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A simple liquid chromatography coupled to quadrupole time of flight mass spectrometry method for macrolide determination in tilapia fillets

Full text
Author(s):
Sismotto, Marcela [1] ; Paschoal, Jonas A. R. [1] ; Teles, Juliana A. [1] ; de Rezende, Renata A. E. [1] ; Reyes, Felix G. R. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Food Composition and Analysis; v. 34, n. 2, p. 153-162, JUN 2014.
Web of Science Citations: 12
Abstract

A method for the identification and quantification of macrolides (erythromycin, josamycin, tilmicosin, tylosin, spiramycin and neospiramycin) in tilapia fillets by liquid chromatography coupled to quadrupole time of flight mass (LC-QToF) spectrometry is presented. Sample preparation was quite simple and low cost: proteins were precipitated and the analytes were extracted with ethanol, extract was defatted with hexane and concentrated by solvent evaporation. The matrix effect was statistically demonstrated during method validation, in which matrix-matched calibration was applied. Matrix effect mechanism was clarified thanks to the capability of QToF mass spectrometer of generating full scan spectra with accurate mass measurement. The limits of quantification were at least 45% lower than the maximum residue limits. The method was able to identify the studied macrolides with relative m/z errors lower than 2.5 ppm and to monitor two fragment ions per analyte, which is in accordance with the European Community recommendations for the analysis of contaminants in foods. Samples from the retail market of Sao Paulo State, Brazil, were analyzed by the developed method and none of them presented positive results for the macrolides studied. (C) 2014 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/07963-3 - Development and validation of analytical method using LC-MS/MS for the determination of macrolides in Nile tilapia (Oreochromis niloticus) meat
Grantee:Felix Guillermo Reyes Reyes
Support Opportunities: Regular Research Grants
FAPESP's process: 08/00456-6 - Macrolides and quinolones in fish: development and validation of analytical methods using LC-MS/MS
Grantee:Jonas Augusto Rizzato Paschoal
Support Opportunities: Scholarships in Brazil - Post-Doctoral