Shifts in phylogenetic diversity of archaeal communities in mangrove sediments at different sites and depths in southeastern Brazil

Author(s)

Mendes, Lucas William ^[1] ; Taketani, Rodrigo Gouvea ^[2] ; Navarrete, Acacio Aparecido ^[3] ; Tsai, Siu Mui ^[4]

Affiliation

^[1] Univ Sao Paulo. Ctr Nucl Energy Agr

^[2] Univ Sao Paulo. Ctr Nucl Energy Agr

^[3] Univ Sao Paulo. Ctr Nucl Energy Agr

^[4] Univ Sao Paulo. Ctr Nucl Energy Agr

• Document type: Journal article
• Source: Research in Microbiology; v. 163, n. 5, p. 366-377, JUN 2012.
• Web of Science Citations: 18

Abstract

This study focused on the structure and composition of archaeal communities in sediments of tropical mangroves in order to obtain sufficient insight into two Brazilian sites from different locations (one pristine and another located in an urban area) and at different depth levels from the surface. Terminal restriction fragment length polymorphism (T-RFLP) of PCR-amplified 16S rRNA gene fragments was used to scan the archaeal community structure, and 16S rRNA gene clone libraries were used to determine the community composition. Redundancy analysis of T-RFLP patterns revealed differences in archaeal community structure according to location, depth and soil attributes. Parameters such as pH, organic matter, potassium and magnesium presented significant correlation with general community structure. Furthermore, phylogenetic analysis revealed a community composition distributed differently according to depth where, in shallow samples, 74.3% of sequences were affiliated with Euryarchaeota and 25.7% were shared between Crenarchaeota and Thaumarchaeota, while for the deeper samples, 24.3% of the sequences were affiliated with Euryarchaeota and 75.7% with Crenarchaeota and Thaumarchaeota. Archaeal diversity measurements based on 16S rRNA gene clone libraries decreased with increasing depth and there was a greater difference between depths (<18% of sequences shared) than sites (>25% of sequences shared). Taken together, our findings indicate that mangrove ecosystems support a diverse archaeal community; it might possibly be involved in nutrient cycles and are affected by sediment properties, depth and distinct locations. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

• FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
• Grantee: Itamar Soares de Melo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants