A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

Author(s)

Gasparotto, Vinicius P. O. ^[1] ; Landim-Alvarenga, Fernanda C. ^{[2, 3]} ; Oliveira, Alexandre L. R. ^{[4, 2]} ; Simoes, Gustavo Ferreira ^[4] ; Lima-Neto, Joao F. ^[2] ; Barraviera, Benedito ^{[2, 1]} ; Ferreira, Jr., Rui S. ^{[2, 1]}

Affiliation

^[1] Univ Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Trop Dis & Image Diag, Botucatu Med Sch, BR-18618970 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, UNESP, Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim UNESP, CEVAP, BR-18610307 Sao Paulo - Brazil

^[3] Univ Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim, BR-18618970 Sao Paulo - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Dept Struct & Funct Biol, Lab Nerve Regenerat, BR-13083865 Sao Paulo - Brazil

• Document type: Journal article
• Source: STEM CELL RESEARCH & THERAPY; v. 5, JUN 10 2014.
• Web of Science Citations: 35

Abstract

Introduction: The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment. Methods: The characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced. Results: The fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival. Conclusions: The new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need. (AU)

• FAPESP's process: 12/08101-8 - In vivo imaging of nerve regeneration following ventral root replantation with fluo-stained fibrin sealant associated with mesenchymal stem cells
• Grantee: Rui Seabra Ferreira Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/06280-0 - Sexual, ontogenetic and enviromental variation in the venom of Crotalus durissus terrificus and Bothrops jararaca on Botucatu region - Sao Paulo State - Brazil: Pharmacological, Biochemical and Enzymatical Characterization.
• Grantee: Rui Seabra Ferreira Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/53846-9 - Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant
• Grantee: Benedito Barraviera
• Support Opportunities: Multi-user Equipment Program