| Full text | |
| Author(s): |
Porcari, Andreia M.
[1]
;
Fernandes, Gabriel D.
[2]
;
Belaz, Katia Roberta A.
[3]
;
Schwab, Nicolas V.
[4]
;
Santos, Vanessa G.
[5]
;
Alberici, Rosana M.
[6]
;
Gromova, Varvara A.
[7]
;
Eberlin, Marcos N.
[8]
;
Lebedev, Albert T.
[9]
;
Tata, Alessandra
[10]
Total Authors: 10
|
| Affiliation: | [1] Univ Estadual Campinas. Inst Chem
[2] Univ Estadual Campinas. Fac Food Engn
[3] Univ Estadual Campinas. Inst Chem
[4] Univ Estadual Campinas. Inst Chem
[5] Univ Estadual Campinas. Inst Chem
[6] Univ Estadual Campinas. Inst Chem
[7] Russian Federat Res Inst Fisheries & Oceanog. Moscow 107140
[8] Univ Estadual Campinas. Inst Chem
[9] Moscow MV Lomonosov State Univ. Organ Chem Dept
[10] Univ Estadual Campinas. Inst Chem
Total Affiliations: 10
|
| Document type: | Journal article |
| Source: | ANALYTICAL METHODS; v. 6, n. 8, p. 2436-2443, 2014. |
| Web of Science Citations: | 15 |
| Abstract | |
The lipid profile of Sturgeon roe (caviar) was monitored by matrix assisted laser desorption/ionization mass spectrometry (MALDI(+)-MS), thermal imprinting easy ambient sonic-spray ionization mass spectrometry (TI-EASI(+)-MS) and electrospray mass spectrometry (ESI(+)-MS). Freshly salted and commercially salted pasteurized caviar samples of Atlantic sturgeon (Acipenser sturio) were stored either at +4 degrees C or at room temperature for 4 weeks. The different types of chemical information achieved by these MS techniques were compared: MALDI(+)-MS detects mainly phospholipids (PL) whereas TI-EASI(+)-MS allows monitoring of both triacylglycerols (TAG) and PL. ESI(+) coupled to Fourier transform ion cyclotron resonance high resolution mass spectrometer (FT-ICR-MS) and MS/MS experiments were used to fully characterize the detected lipids, ensure the absence of oxidation products in the degradation process and confirm the high efficiency of the thermal imprinting extraction. TI-EASI(+)-MS, via a more comprehensive profiling and easier operation, has therefore been demonstrated to provide caviar lipidomic profiles and discriminate its changes as a function of storage time and temperature. The data have also confirmed that hydrolysis is the main process of lipid degradation in caviar. (AU) | |
| FAPESP's process: | 11/06191-7 - MALDI-TOF and ESI-MS fingerprinting for bacteria identification in culture media and semen as a tool for sanitary excelence in bovine embryo in vitro production |
| Grantee: | Alessandra Tata |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/07206-0 - Search for a Molecular Signature of Receptivity in Cattle by Mass Spectrometry |
| Grantee: | Katia Roberta Anacleto Belaz |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |