Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles

Full text
Author(s):
Buffoni Campos Carneiro, Lara Aparecida [1] ; Costa-Silva, Tales Alexandre [1] ; Fernandes Souza, Claudia Regina [1] ; Bachmann, Luciano [2] ; Oliveira, Wanderley Pereira [1] ; Said, Suraia [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14049 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Archives of Biology and Technology; v. 57, n. 4, p. 578-586, JUL-AUG 2014.
Web of Science Citations: 4
Abstract

This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of storage at 4°C, while lipases immobilized on pre-activated CAM maintained its activity up to 180 days at same conditions. Taken together the results indicate that chitosan microparticles provide an optimal microenvironment for the immobilized enzyme to maintain good activity and stability. (AU)

FAPESP's process: 11/00743-8 - Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications
Grantee:Tales Alexandre da Costa e Silva
Support Opportunities: Scholarships in Brazil - Doctorate