Assessment of Bacterial bph Gene in Amazonian Dark Earth and Their Adjacent Soils

Author(s)

de Lima Brossi, Maria Julia ^[1] ; Mendes, Lucas William ^[2] ; Germano, Mariana Gomes ^[3] ; Lima, Amanda Barbosa ^[4] ; Tsai, Siu Mui ^[5]

Affiliation

^[1] Univ Sao Paulo. Ctr Nucl Energy Agr

^[2] Univ Sao Paulo. Ctr Nucl Energy Agr

^[3] Embrapa Soybean. Brazilian Agr Res Corp

^[4] Univ Sao Paulo. Ctr Nucl Energy Agr

^[5] Univ Sao Paulo. Ctr Nucl Energy Agr

• Document type: Journal article
• Source: PLoS One; v. 9, n. 6 JUN 13 2014.
• Web of Science Citations: 1

Abstract

Amazonian Anthrosols are known to harbour distinct and highly diverse microbial communities. As most of the current assessments of these communities are based on taxonomic profiles, the functional gene structure of these communities, such as those responsible for key steps in the carbon cycle, mostly remain elusive. To gain insights into the diversity of catabolic genes involved in the degradation of hydrocarbons in anthropogenic horizons, we analysed the bacterial bph gene community structure, composition and abundance using T-RFLP, 454-pyrosequencing and quantitative PCR essays, respectively. Soil samples were collected in two Brazilian Amazon Dark Earth (ADE) sites and at their corresponding non-anthropogenic adjacent soils (ADJ), under two different land use systems, secondary forest (SF) and manioc cultivation (M). Redundancy analysis of T-RFLP data revealed differences in bph gene structure according to both soil type and land use. Chemical properties of ADE soils, such as high organic carbon and organic matter, as well as effective cation exchange capacity and pH, were significantly correlated with the structure of bph communities. Also, the taxonomic affiliation of bph gene sequences revealed the segregation of community composition according to the soil type. Sequences at ADE sites were mostly affiliated to aromatic hydrocarbon degraders belonging to the genera Streptomyces, Sphingomonas, Rhodococcus, Mycobacterium, Conexibacter and Burkholderia. In both land use sites, shannon's diversity indices based on the bph gene data were higher in ADE than ADJ soils. Collectively, our findings provide evidence that specific properties in ADE soils shape the structure and composition of bph communities. These results provide a basis for further investigations focusing on the bio-exploration of novel enzymes with potential use in the biotechnology/biodegradation industry. (AU)

• FAPESP's process: 11/50914-3 - The microbiome of Amazonian dark earth: structure and function of the microbial communities from rhizosphere and biochar associated to the biogeochemical cycles
• Grantee: Tsai Siu Mui
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants