Meta-analysis of Methylenetetrahydrofolate reductase maternal gene in Down syndrome: increased susceptibility in women carriers of the MTHFR 677T allele

Author(s)

Victorino, D. B. ^[1] ; Godoy, M. F. ^[2] ; Goloni-Bertollo, E. M. ^[1] ; Pavarino, E. C. ^[1]

Affiliation

^[1] Fac Med Sao Jose Rio Preto FAMERP, Unidade Pesquisa Genet & Biol Mol UPGEM, BR-15090000 Sao Paulo - Brazil

^[2] Fac Med Sao Jose Rio Preto FAMERP, Nucleo Transdisciplinar Estudo Caos & Complexidad, BR-15090000 Sao Paulo - Brazil

• Document type: Journal article
• Source: MOLECULAR BIOLOGY REPORTS; v. 41, n. 8, p. 5491-5504, AUG 2014.
• Web of Science Citations: 4

Abstract

Because a number of data studies include some controversial results about Methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Down syndrome (DS), we performed a meta-analysis to determine a more precise estimation of this association. Studies were searched on PubMed, EMBASE and Lilacs-Scielo, up to April 2013, and they were eligible if they included case mothers (DSM) that have gave birth to children with DS, and controls mothers (CM) that have gave birth to healthy children without chromosomal abnormality, syndrome or malformation. The combined odds ratio with 95 % confidence intervals was calculated by fixed or random effects models to assess the strength of associations. Potential sources of heterogeneity between studies were evaluated using Q test and the I-2. Publication bias was estimated using Begg's test and Egger's linear regression test. Sensitivity analyses were performed by using allelic, dominant, recessive and codominant genetic models, Hardy-Weinberg equilibrium (HWE) and ethnicity. Twenty-two studies with 2,223 DSM and 2,807 CM were included for MTHFR C677T and 15 studies with 1,601 DSM and 1,849 CM were included for MTHFR A1298C. Overall analysis suggests an association of the MTHFR C677T polymorphism with maternal risk for DS. Moreover, no association between the MTHFR A1298C polymorphism and maternal risk for DS was found. There is also evidence of higher heterogeneity, with I-2 test values ranging from 8 to 89 %. No evidence of publication bias was found. Taken together, our meta-analysis implied that the T allele carriers might carry an increased maternal risk for DS. (AU)

• FAPESP's process: 12/04713-9 - A SYSTEMATIC REVIEW AND META-ANALYSIS OF THE ASSOCIATION BETWEEN POLYMORPHISMS IN MATERNAL GENES INVOLVED IN FOLATE METABOLISM AND THE BIRTH OF INDIVIDUALS WITH DOWN SYNDROME.
• Grantee: Daniella Balduino Victorino
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/04664-8 - A systematic review and meta-analysis of the association between polymorphisms in maternal genes involved in folate metabolism and the birth of individuals with Down Syndrome
• Grantee: Erika Cristina Pavarino
• Support Opportunities: Regular Research Grants