| Full text | |
| Author(s): |
Evaristo, Thaiane Cristine
[1]
;
Maciel da CruzAlves, Flavia Cilene
[2]
;
Moroz, Andrei
[3]
;
Mion, Woner
[4]
;
Acorci-Valerio, Michele Janegitz
[5]
;
Felisbino, Sergio Luis
[6]
;
Rossi-Ferreira, Rosana
[7]
;
Ruiz, Jr., Raul Lopes
[8]
;
Deffune, Elenice
[9]
Total Authors: 9
|
| Affiliation: | [1] Paulista State Univ UNESP, Sch Med, Dept Surg & Orthopedy, Postgrad Program Gen Basis Surg, Botucatu, SP - Brazil
[2] Herminio Ometto Univ Ctr, Grad Program Biomed Sci, Araras, SP - Brazil
[3] UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, SP - Brazil
[4] UNESP, Sch Med, Ctr Blood Transfus, Cell Engn Lab, Botucatu, SP - Brazil
[5] Paulista Univ UNIP, Dept Hlth Sci, Bauru, SP - Brazil
[6] UNESP, Biosci Inst, Dept Morphol, Botucatu, SP - Brazil
[7] UNESP, Fac Sci, Dept Sci Biol, Bauru, SP - Brazil
[8] UNESP, Sch Med, Dept Surg & Orthopedy, Botucatu, SP - Brazil
[9] UNESP, Sch Med, Dept Urol, Botucatu, SP - Brazil
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | Acta Cirurgica Brasileira; v. 29, n. 8, p. 485-492, AUG 2014. |
| Web of Science Citations: | 7 |
| Abstract | |
PURPOSE: To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS:Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION:The light-emitting diode is a promising tool for decellularization of soft tissues. (AU) | |