Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos

• Processo: 15/13684-0
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Data de Início da vigência: 01 de março de 2017
• Data de Término da vigência: 28 de fevereiro de 2023
• Área do conhecimento: Ciências Biológicas - Biofísica - Biofísica Molecular
• Pesquisador responsável: Igor Polikarpov
• Beneficiário: Igor Polikarpov
• Instituição Sede: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brasil
• Município da Instituição Sede: São Carlos
• Pesquisadores principais: Francisco Eduardo Gontijo Guimaraes
• Pesquisadores associados: Alessandro Silva Nascimento ; Camila Alves de Rezende ; Eduardo Ribeiro de Azevedo ; Marcos Silveira Buckeridge ; Munir Salomao Skaf
• Auxílio(s) vinculado(s): 18/22300-0 - Aumento da oxidação enzimática de lignocelulose através de atividades sinérgicas usando luz e LPMOs, AP.R SPRINT
• Bolsa(s) vinculada(s): 21/13009-2 - Estudos estruturais e funcionais de enzimas que participam na sintese e degradação de carboidratos complexos, BP.PD; 20/15588-7 - Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos, BP.TT; 19/19360-3 - Hidrogéis nanocompósitos inspirados na parede celular vegetal para aplicações biomédicas, BP.DR; + mais bolsas vinculadas 19/21116-3 - Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos, BP.TT; 19/10942-0 - Produção heteróloga e caracterização da xilanases de famílias GH10 e GH11 e avaliação das suas aplicações na degradação de xilano e obtenção de xilooligossacarídeos a partir da biomassa lignocelulósica, BP.IC; 18/24705-7 - Estudos estruturais e funcionais de enzimas que participam na sintese e degradação de carboidratos complexos, BP.PD; 18/00082-0 - Estudos estruturais e bioquímicos de enzimas envolvidas na degradação de glucuroarabinoxilano (GAX), BP.IC; 18/02028-3 - Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos, BP.TT; 17/20706-6 - Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos, BP.TT - menos bolsas vinculadas
• Assunto(s): Cristalografia de proteínas Hidrolases Glicosídeos Hidrólise Parede celular vegetal Biocombustíveis
• Palavra(s)-Chave do Pesquisador: carboidratos complexos | Cristalografia de Proteínas | Hidrolases e Transferases de glicosídeos | Hidrolise de parede celular vegetal | Síntese de parede celular vegetal | Biologia Molecular Estrutural

Resumo

Os carboidratos, como mono- e oligossacarídeos, polissacarídeos e glicoconjugados, desempenham papéis extremamente importantes em todas as formas de vida, participando em interações patógeno-hospedeiro, transdução de sinais, inflamação, tráfego intracelular e diferenciação. Além disso, os carboidratos correspondem a cerca de três quartos da biomassa renovável das plantas (~135 bilhões de toneladas/ano) cujas paredes celulares e açúcares de reserva representam uma fonte renovável de energia e materiais "verdes". Entretanto, a diversidade dos carboidratos e a complexidade da parede celular vegetal tornam a disponibilização enzimática de açúcares simples para produção de biocombustíveis e produtos químicos "verdes" uma tarefa difícil. A nossa atual compreensão de síntese e degradação enzimática de carboidratos complexos no contexto de parede celular está no mínimo incompleta. Estima-se que atualmente menos de 3,8% de enzimas e proteínas auxiliares envolvidas na síntese e degradação de carboidratos estão caracterizadas bioquimicamente e menos de 0,42% estão caracterizadas estruturalmente (Lombard et al., Nucl. Acids Res. (2014) 42: D490-D495). Portanto, neste projeto propomos um estudo estrutural e funcional das enzimas com potencial de depolimerização e síntese de polissacarídeos, visando uma melhor compreensão da sua especificidade e dos mecanismos de ação no contexto da parede celular e sobre carboidratos isolados. Os resultados deste estudo vão trazer conhecimentos básicos em relação da ação enzimática sobre biopolímeros e podem ser importantes para aplicações biotecnológicas como a hidrólise enzimática de materiais lignocelulósicos e transformação de carboidratos em compostos químicos "verdes" e biocombustíveis, entre outras. (AU)

Publicações científicas

(65)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

EVANGELISTA, DANILO ELTON; DE ARAUJO, EVANDRO A.; OLIVEIRA NETO, MARIO; SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS structure of an exopolygalacturonase from Bacillus licheniformis. NEW BIOTECHNOLOGY, v. 40, n. B, p. 268-274, JAN 25 2017. (11/20505-4, 15/13684-0)

DE ARAUJO, EVANDRO ARES; DE OLIVEIRA NETO, MARIO; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS structure of two-domain endoglucanase BlCel9 from Bacillus licheniformis. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1275-1287, FEB 2019. (15/13684-0)

MELLO, BRUNO L.; ALESSI, ANNA M.; RIANO-PACHON, DIEGO M.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. G.; ESPIRITO SANTO, MELISSA C.; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-beta-xylanase activity. BIOTECHNOLOGY FOR BIOFUELS, v. 10, NOV 2 2017. (11/21608-1, 10/52362-5, 15/13684-0)

SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Structural insights into the hydrolysis pattern and molecular dynamics simulations of GH45 subfamily a endoglucanase from Neurospora crassa OR74A. Biochimie, v. 165, p. 275-284, OCT 2019. (15/13684-0, 18/11730-3)

DO ESPIRITO SANTO, MELISSA CRISTINA; CARDOSO, ELIANO BRITO; GONTIJO GUIMARAES, FRANCISCO EDUARDO; DEAZEVEDO, EDUARDO RIBEIRO; DA CUNHA, GIOVANNI PARO; NOVOTNY, ETELVINO HENRIQUE; ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; CHANDEL, ANUJ KUMAR; LUCIANO SILVEIRA, MARCOS HENRIQUE; POLIKARPOV, IGOR. Multifaceted characterization of sugarcane bagasse under different steam explosion severity conditions leading to distinct enzymatic hydrolysis yields. INDUSTRIAL CROPS AND PRODUCTS, v. 139, NOV 1 2019. (15/13684-0)

HANS, MEENU; KUMAR, SACHIN; CHANDEL, ANUJ KUMAR; POLIKARPOV, IGOR. A review on bioprocessing of paddy straw to ethanol using simultaneous saccharification and fermentation. Process Biochemistry, v. 85, p. 125-134, OCT 2019. (15/13684-0)

FARRO, ERICK GIANCARLO S.; LEITE, ANA ELISA T.; SILVA, ISABELA A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; POLIKARPOV, IGOR; NASCIMENTO, ALESSANDRO S.. GH43 endo-arabinanase from Bacillus licheniformis: Structure, activity and unexpected synergistic effect on cellulose enzymatic hydrolysis. International Journal of Biological Macromolecules, v. 117, p. 7-16, OCT 1 2018. (14/06565-2, 15/13684-0, 14/03768-0)

SEIKI KADOWAKI, MARCO ANTONIO; RABELO HIGASI, PAULA MIWA; DE GODOY, MARIANA ORTIZ; DE ARAUJO, EVANDRO ARES; GODOY, ANDRE SCHUTZER; PRADE, ROLF ALEXANDER; POLIKARPOV, IGOR. Enzymatic versatility and thermostability of a new aryl-alcohol oxidase from Thermothelomyces thermophilus M77. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1864, n. 10, OCT 2020. (15/13684-0, 11/20505-4)

BESSER, KATRIN; MALYON, GRAHAM P.; EBORALL, WILLIAM S.; DA CUNHA, GIOVANNI PARO; FILGUEIRAS, JEFFERSON G.; DOWLE, ADAM; GARCIA, LOURDES CRUZ; PAGE, SAMUEL J.; DUPREE, RAY; KERN, MARCELO; et al. Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans. NATURE COMMUNICATIONS, v. 9, DEC 3 2018. (15/13684-0, 17/24465-3)

BERTO, GABRIELA LEILA; VELASCO, JOSMAN; CABOS RIBEIRO, CAIO TASSO; ZANPHORLIN, LETICIA MARIA; DOMINGUES, MARIANE NORONHA; MURAKAMI, MARIO TYAGO; POLIKARPOV, IGOR; DE OLIVEIRA, LEANDRO CRISTANTE; FERRAZ, ANDRE; SEGATO, FERNANDO. Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45. Enzyme and Microbial Technology, v. 120, p. 23-35, JAN 2019. (14/06923-6, 14/18714-2, 15/13684-0, 15/26982-0)

SARTORI, GERALDO RODRIGUES; NASCIMENTO, ALESSANDRO S.. Comparative Analysis of Electrostatic Models for Ligand Docking. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 6, JUL 3 2019. (15/13684-0, 17/18173-0, 15/26722-8)

KANE, AISSATA OUSMANE; SCOPEL, EUPIDIO; CORTEZ, ANELYSE ABREU; ROSSI, BRUNO ROBERTO; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; REZENDE, CAMILA ALVES; POLIKARPOV, IGOR. Enzyme-assisted production of cellulose nanofibers from bleached and bleached/sulfonated sugarcane bagasse: impact of sulfonation on nanocellulose properties and yields. Cellulose, v. 30, n. 18, p. 16-pg., 2023-11-18. (19/19360-3, 21/08780-1, 15/13684-0, 21/12071-6, 18/23769-1)

VELDMAN, WAYDE; LIBERATO, MARCELO VIZONA; ALMEIDA, VITOR M.; SOUZA, VALQUIRIA P.; FRUTUOSO, MAIRA A.; MARANA, SANDRO R.; MOSES, VUYANI; BISHOP, OZLEM TASTAN; POLIKARPOV, IGOR. X-ray Structure, Bioinformatics Analysis, and Substrate Specificity of a 6-Phospho-beta-glucosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 60, n. 12, p. 6392-6407, DEC 28 2020. (15/13684-0)

SEPULCHRO, V, ANA GABRIELA; PELLEGRINI, VANESSA O. A.; DIAS, LUCAS D.; KADOWAKI, MARCO A. S.; CANNELLA, DAVID; POLIKARPOV, IGOR. Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A. BIOFUEL RESEARCH JOURNAL-BRJ, v. 8, n. 3, p. 1454-1464, SUM 2021. (18/22300-0, 15/13684-0, 19/13569-8)

DE AZEVEDO, ERIKA CHANG; NASCIMENTO, ALESSANDRO S.. Energy landscape of the domain movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase. Journal of Structural Biology, v. 207, n. 2, p. 158-168, AUG 1 2019. (15/13684-0, 17/18173-0, 15/26722-8)

EVANGELISTA, DANILO ELTON; SEIKI KADOWAKI, MARCO ANTONIO; MELLO, BRUNO LUAN; POLIKARPOV, IGOR. Biochemical and biophysical characterization of novel GH10 xylanase prospected from a sugar cane bagasse compost-derived microbial consortia. International Journal of Biological Macromolecules, v. 109, p. 560-568, APR 2018. (11/20505-4, 11/21608-1, 10/52362-5, 15/13684-0)

VIEIRA, PLINIO S.; BONFIM, ISABELA M.; ARAUJO, EVANDRO A.; MELO, RICARDO R.; LIMA, AUGUSTO R.; FESSEL, MELISSA R.; PAIXAO, DOUGLAS A. A.; PERSINOTI, GABRIELA F.; ROCCO, SILVANA A.; LIMA, TATIANI B.; et al. Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors. NATURE COMMUNICATIONS, v. 12, n. 1, JUN 30 2021. (17/14253-9, 15/26982-0, 16/06509-0, 15/13684-0, 18/03724-3, 19/13936-0, 17/00203-0, 16/19995-0)

KADOWAKI, MARCO A. S.; BRIGANTI, LORENZO; EVANGELISTA, DANILO E.; ECHEVARRIA-POZA, ALBERTO; TRYFONA, THEODORA; PELLEGRINI, VANESSA O. A.; NAKAYAMA, DARLAN G.; DUPREE, PAUL; POLIKARPOV, IGOR. Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost-derived consortium. Biotechnology and Bioengineering, v. 118, n. 10, JUL 2021. (15/13684-0)

KANE, AISSATA OUSMANE; PELLERGINI, VANESSA O. ARNOLDI; ESPIRITO SANTO, MELISSA C.; NGOM, BALLA DIOP; GARCIA, JOSE M.; ACEVEDO, ALBERTO; ERAZZU, LUIS E.; POLIKARPOV, IGOR. valuating the Potential of Culms from Sugarcane and Energy Cane Varieties Grown in Argentina for Second-Generation Ethanol Productio. WASTE AND BIOMASS VALORIZATION, v. 13, n. 1, SI, JUL 2021. (15/13684-0)

BARRA, ANGELICA LUANA C.; ULLAH, NAJEEB; MORAO, LUANA G.; WRENGER, CARSTEN; BETZEL, CHRISTIAN; NASCIMENTO, ALESSANDRO S.. Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes in Plasmodium: Advances and Open Questions. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUL 13 2021. (18/21213-6, 19/20219-3, 20/03983-9, 15/13684-0, 19/26428-3, 15/26722-8, 17/03966-4)

ESPIRITO SANTO, MELISSA C.; KANE, AISSATA OUSMANE; PELLEGRINI, VANESSA O. A.; THEMA, FORCE TEFO; GARCIA, JOSE M.; ACEVEDO, ALBERTO; ERAZZU, LUIS E.; GUIMARAES, FRANCISCO E. G.; DEAZEVEDO, EDUARDO R.; POLIKARPOV, IGOR. Leaves from four different sugarcane varieties as potential renewable feedstocks for second-generation ethanol production: Pretreatments, chemical composition, physical structure, and enzymatic hydrolysis yields. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 45, p. 13-pg., 2022-10-01. (15/13684-0)

VELDMAN, WAYDE; LIBERATO, MARCELO VIZONA; SOUZA, VALQUIRIA P.; ALMEIDA, VITOR M.; MARANA, SANDRO R.; BISHOP, OZLEM TASTAN; POLIKARPOV, IGOR. Differences in Gluco and Galacto Substrate-Binding Interactions in a Dual 6P beta-Glucosidase/6P beta-Galactosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 61, n. 9, p. 4554-4570, SEP 27 2021. (15/13684-0)

KANE, AISSATA OUSMANE; CORTEZ, ANELYSE ABREU; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; NGOM, BALLA DIOP; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; POLIKARPOV, IGOR. Combined liquid hot water and sulfonation pretreatment of sugarcane bagasse to maximize fermentable sugars production. INDUSTRIAL CROPS AND PRODUCTS, v. 201, p. 13-pg., 2023-05-23. (15/13684-0, 21/08780-1)

VACILOTTO, MILENA MOREIRA; ARNOLDI PELLEGRINI, VANESSA O.; VEIGA SEPULCHRO, ANA GABRIELA; DE MELLO CAPETTI, CAIO C.; CURVELO, ANTONIO APRIGIO S.; MARCONDES, WILIAN FIORELI; ARANTES, VALDEIR; POLIKARPOV, IGOR. Paludibacter propionicigenes GH10 xylanase as a tool for enzymatic xylooligosaccharides production from heteroxylans. Carbohydrate Polymers, v. 275, JAN 1 2022. (15/13684-0)

DE AZEVEDO, ERIKA CHANG; NASCIMENTO, ALESSANDRO S.. The beta-lactam ticarcillin is a Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase binder. Biochimie, v. 197, p. 8-pg., 2022-02-03. (17/18173-0, 15/26722-8, 15/13684-0, 20/03983-9)

ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; VEIGA SEPULCHRO, ANA GABRIELA; POLIKARPOV, IGOR. Enzymes for lignocellulosic biomass polysaccharide valorization and production of nanomaterials. CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY, v. 26, DEC 2020. (15/13684-0)

SCOPEL, EUPIDIO; PINTO, LIDIANE O.; REZENDE, CAMILA A.. Single-step bleaching versus organosolv-bleaching of sugarcane bagasse: tuning TEMPO-oxidized nanocellulose morphology via delignification strategy. International Journal of Biological Macromolecules, v. 325, p. 10-pg., 2025-09-25. (21/12071-6, 23/01620-4, 15/13684-0, 19/19360-3)

PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; RATTI, REGIANE PRISCILA; FILGUEIRAS, JEFFERSON GONCALVES; FALVO, MAURICIO; CORAL, MARISA APARECIDA LIMA; GUIMARAES, FRANCISCO EDUARDO GONTIJO; DEAZEVEDO, EDUARDO RIBEIRO; POLIKARPOV, IGOR. Differences in chemical composition and physical properties caused by industrial storage on sugarcane bagasse result in its efficient enzymatic hydrolysis. SUSTAINABLE ENERGY & FUELS, v. 6, n. 2, NOV 2021. (15/13684-0)

HANS, MEENU; PELLEGRINI, VANESSA O. A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. C.; CHANDEL, ANUJ KUMAR; POLIKARPOV, IGOR; CHADHA, BHUPINDER SINGH; KUMAR, SACHIN. Optimization of Dilute Acid Pretreatment for Enhanced Release of Fermentable Sugars from Sugarcane Bagasse and Validation by Biophysical Characterization. BioEnergy Research, v. N/A, p. 19-pg., 2022-05-19. (15/13684-0)

KADOWAKI, MARCO A. S.; VARNAI, ANIKO; JAMESON, JOHN-KRISTIAN; LEITE, ANA E. T.; COSTA-FILHO, ANTONIO J.; KUMAGAI, PATRICIA S.; PRADE, ROLF A.; POLIKARPOV, IGOR; EIJSINK, VINCENT G. H.. Functional characterization of a lytic polysaccharide monooxygenase from the thermophilic fungus Myceliophthora thermophila. PLoS One, v. 13, n. 8, p. 16-pg., 2018-08-20. (11/20505-4, 15/13684-0)

ARAUJO, EVANDRO A.; DIAS, ARTUR HERMANO SAMPAIO; KADOWAKI, MARCO A. S.; PIYADOV, VASILY; PELLEGRINI, VANESSA O. A.; URIO, MATEUS B.; RAMOS, LUIZ P.; SKAF, MUNIR S.; POLIKARPOV, IGOR. mpact of cellulose properties on enzymatic degradation by bacterial GH48 enzymes: Structural and mechanistic insights from processive Bacillus licheniformis Cel48B cellulas. Carbohydrate Polymers, v. 264, JUL 15 2021. (13/08293-7, 15/13684-0, 14/00769-5, 19/17350-0)

SANTO, MELISSA ESPIRITO; REZENDE, CAMILA A.; BERNARDINELLI, OIGRES D.; PEREIRA, JR., NEI; CURVELO, ANTONIO A. S.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANSCISCO E. G.; POLIKARPOV, IGOR. Structural and compositional changes in sugarcane bagasse subjected to hydrothermal and organosolv pretreatments and their impacts on enzymatic hydrolysis. INDUSTRIAL CROPS AND PRODUCTS, v. 113, p. 64-74, MAR 2018. (10/52362-5, 15/13684-0)

PELLEGRINI, VANESSA O. A.; BERNARDES, AMANDA; REZENDE, CAMILA A.; POLIKARPOV, IGOR. Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases. Cellulose, v. 25, n. 3, p. 1865-1881, MAR 2018. (12/22802-9, 10/18773-8, 15/13684-0, 16/13602-7)

DE ARAUJO, EVANDRO ARES; MANZINE, LIVIA REGINA; PIIADOV, VASSILI; SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Biochemical characterization, low-resolution SAXS structure and an enzymatic cleavage pattern of BlCel48 from Bacillus licheniformis. International Journal of Biological Macromolecules, v. 111, p. 302-310, MAY 2018. (14/00769-5, 11/20505-4, 10/52362-5, 15/13684-0)

GODOY, ANDRE S.; PEREIRA, CAROLINE S.; RAMIA, MARINA PAGLIONE; SILVEIRA, RODRIGO L.; CAMILO, CESAR M.; KADOWAKI, MARCO A.; LANGE, LENE; BUSK, PETER K.; NASCIMENTO, ALESSANDRO S.; SKAF, MUNIR S.; et al. Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members. SCIENTIFIC REPORTS, v. 8, FEB 27 2018. (15/13684-0, 11/21608-1, 10/52362-5, 11/20505-4)

KADOWAKI, MARCO A. S.; HIGASI, PAULA; DE GODOY, MARIANA O.; PRADE, ROLF A.; POLIKARPOV, IGOR. Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila. FEBS Journal, v. 285, n. 3, p. 559-579, FEB 2018. (09/52840-7, 15/13684-0, 11/20505-4)

PUHL, ANA C.; PRATES, ERICA T.; ROSSETO, FLAVIO R.; MANZINE, LIVIA R.; STANKOVIC, IVANA; DE ARAUJO, SIMARA S.; ALVAREZ, THABATA M.; SQUINA, FABIO M.; SKAF, MUNIR S.; POLIKARPOV, IGOR. Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern. International Journal of Biological Macromolecules, v. 136, p. 493-502, SEP 1 2019. (11/20505-4, 15/13684-0, 09/52840-7, 10/52362-5, 11/21608-1, 15/50590-4)

EVANGELISTA, DANILO ELTON; ARNOLDI PELLEGRIN, VANESSA DE OLIVEIRA; SANTO, MELISSA ESPIRITO; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS shape of a novel GH11 exo-1,4-beta-xylanase identified in a microbial consortium. Applied Microbiology and Biotechnology, v. 103, n. 19, p. 8035-8049, OCT 2019. (15/13684-0, 11/21608-1, 10/52362-5, 11/20505-4)

BRAR, KAMALPREET KAUR; SANTO, MELISSA C. ESPIRITO; PELLEGRINI, VANESSA O. A.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. C.; POLIKARPOV, IGOR; CHADHA, BHUPINDER SINGH. Enhanced hydrolysis of hydrothermally and autohydrolytically treated sugarcane bagasse and understanding the structural changes leading to improved saccharification. BIOMASS & BIOENERGY, v. 139, AUG 2020. (15/13684-0)

LEITE, ANA ELISA T.; BRIGANTI, LORENZO; DE ARAUJO, EVANDRO ARES; ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; CAMILO, CESAR MOYSES; POLIKARPOV, IGOR. Low-resolution molecular shape, biochemical characterization and emulsification properties of a halotolerant esterase fromBacillus licheniformis. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, v. 49, n. 6, JUL 2020. (15/13684-0)

TOMAZINI, ATILIO; HIGASI, PAULA; MANZINE, LIVIA R.; STOTT, MATTHEW; SPARLING, RICHARD; LEVIN, DAVID B.; POLIKARPOV, IGOR. A novel thermostable GH5 beta-xylosidase from Thermogemmatispora sp. T81. NEW BIOTECHNOLOGY, v. 53, p. 57-64, NOV 25 2019. (15/13684-0)

KELLER, M. B.; FELBY, C.; LABATE, C. A.; PELLEGRINI, V. O. A.; HIGASI, P.; SINGH, R. K.; POLIKARPOV, I.; BLOSSOM, B. M.. A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases. Biotechnology Letters, v. 42, n. 1, p. 93-102, JAN 2020. (17/01165-4, 15/13684-0)

PIIADOV, VASSILI; DE ARAUJO, EVANDRO ARES; OLIVEIRA NETO, MARIO; CRAIEVICH, ALDO FELIX; POLIKARPOV, IGOR. SAXSMoW 2.0: Online calculator of the molecular weight of proteins in dilute solution from experimental SAXS data measured on a relative scale. Protein Science, v. 28, n. 2, p. 454-463, FEB 2019. (14/00769-5, 15/13684-0)

KADOWAKI, MARCO A. S.; VARNAI, ANIKO; JAMESON, JOHN-KRISTIAN; LEITE, ANA E. T.; COSTA-FILHO, ANTONIO J.; KUMAGAI, PATRICIA S.; PRADE, ROLF A.; POLIKARPOV, IGOR; EIJSINK, VINCENT G. H.. Functional characterization of a lytic polysaccharide monooxygenase from the thermophilic fungus Myceliophthora thermophila. PLoS One, v. 13, n. 8, AUG 20 2018. (11/20505-4, 15/13684-0)

ESPIRITO SANTO, MELISSA C.; FOCKINK, DOUGLAS H.; PELLEGRINI, VANESSA O. A.; GUIMARAES, FRANCISCO E. G.; DEAZEVEDO, EDUARDO R.; RAMOS, LUIZ PEREIRA; POLIKARPOV, IGOR. Physical techniques shed light on the differences in sugarcane bagasse structure subjected to steam explosion pretreatments at equivalent combined severity factors. INDUSTRIAL CROPS AND PRODUCTS, v. 158, DEC 15 2020. (15/13684-0)

VELASCO, JOSMAN; SEPULCHRO, ANA GABRIELA V.; HIGASI, PAULA M. R.; PELLEGRINI, VANESSA O. A.; CANNELLA, DAVID; DE OLIVEIRA, LEANDRO CRISTANTE; POLIKARPOV, IGOR; SEGATO, FERNANDO. Light Boosts the Activity of Novel LPMO from Aspergillus fumigatus Leading to Oxidative Cleavage of Cellulose and Hemicellulose. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 10, n. 50, p. 16-pg., 2022-12-08. (19/22284-7, 18/22300-0, 19/01165-0, 21/06679-1, 14/18714-2, 15/13684-0)

ARAUJO, EVANDRO A.; DIAS, ARTUR HERMANO SAMPAIO; KADOWAKI, MARCO A. S.; PIYADOV, VASILY; PELLEGRINI, VANESSA O. A.; URIO, MATEUS B.; RAMOS, LUIZ P.; SKAF, MUNIR S.; POLIKARPOV, IGOR. Impact of cellulose properties on enzymatic degradation by bacterial GH48 enzymes: Structural and mechanistic insights from processive Bacillus licheniformis Cel48B cellulase. Carbohydrate Polymers, v. 264, p. 13-pg., 2021-04-12. (14/00769-5, 13/08293-7, 15/13684-0, 19/17350-0)

EVANGELISTA, DANILO ELTON; DE ARAUJO, EVANDRO A.; OLIVEIRA NETO, MARIO; SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS structure of an exopolygalacturonase from Bacillus licheniformis. NEW BIOTECHNOLOGY, v. 40, p. 7-pg., 2018-01-25. (15/13684-0, 11/20505-4)

BRIGANTI, LORENZO; CAPETTI, CAIO; PELLEGRINI, VANESSA O. A.; GHIO, SILVINA; CAMPOS, ELEONORA; NASCIMENTO, ALESSANDRO S.; POLIKARPOV, IGOR. Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v. 19, p. 1557-1566, 2021. (20/03983-9, 15/26722-8, 15/13684-0)

CAPETTI, CAIO CESAR DE MELLO; VACILOTTO, MILENA MOREIRA; DABUL, ANDREI NICOLI GEBIELUCA; SEPULCHRO, ANA GABRIELA VEIGA; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; POLIKARPOV, IGOR. Recent advances in the enzymatic production and applications of xylooligosaccharides. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 37, n. 10, OCT 2021. (15/13684-0)

HANS, MEENU; GARG, SHRUTI; PELLEGRINI, VANESSA O. A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. C.; CHANDEL, ANUJ KUMAR; POLIKARPOV, IGOR; CHADHA, BHUPINDER SINGH; KUMAR, SACHIN. Liquid ammonia pretreatment optimization for improved release of fermentable sugars from sugarcane bagasse. JOURNAL OF CLEANER PRODUCTION, v. 281, JAN 25 2021. (15/13684-0)

ROSSI, BRUNO R.; PELLEGRINI, VANESSA O. A.; CORTEZ, ANELYSE A.; CHIROMITO, EMANOELE M. S.; CARVALHO, ANTONIO J. F.; PINTO, LIDIANE O.; REZENDE, CAMILA A.; MASTELARO, VALMOR R.; POLIKARPOV, IGOR. Cellulose nanofibers production using a set of recombinant enzymes. Carbohydrate Polymers, v. 256, MAR 15 2021. (15/13684-0)

VACILOTTO, MILENA MOREIRA; SEPULCHRO, ANA GABRIELA VEIGA; PELLEGRINI, VANESSA O. A.; POLIKARPOV, IGOR. Production of prebiotic xylooligosaccharides from arabino- and glucuronoxylan using a two-domain Jonesia denitrificans xylanase from GH10 family. Enzyme and Microbial Technology, v. 144, MAR 2021. (15/13684-0)

VELASCO, JOSMAN; PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; SEPULCHRO, ANA GABRIELA VEIGA; KADOWAKI, MARCO ANTONIO SEIKI; SANTO, MELISSA CRISTINA ESPIRITO; POLIKARPOV, IGOR; SEGATO, FERNANDO. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification. Enzyme and Microbial Technology, v. 144, MAR 2021. (19/22284-7, 15/13684-0, 14/06923-6, 14/18714-2, 19/01165-0, 17/50025-0)

DO ESPIRITO SANTO, MELISSA CRISTINA; THEMA, FORCE TEFO; ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; KANE, AISSATA OUSMANE; GONTIJO GUIMARAES, FRANCISCO EDUARDO; FILGUEIRAS, JEFFERSON G.; NOVOTNY, ETELVINO HENRIQUE; DEAZEVEDO, EDUARDO RIBEIRO; POLIKARPOV, IGOR. When the order matters: Impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse. INDUSTRIAL CROPS AND PRODUCTS, v. 180, p. 12-pg., 2022-03-07. (15/13684-0)

CAPETTI, CAIO CESAR DE MELLO; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; SANTO, MELISSA CRISTINA ESPIRITO; CORTEZ, ANELYSE ABREU; FALVO, MAURICIO; CURVELO, ANTONIO APRIGIO DA SILVA; CAMPOS, ELEONORA; FILGUEIRAS, JEFFERSON GONSALVES; GUIMARAES, FRANCISCO EDUARDO GONTIJO; DE AZEVEDO, EDUARDO RIBEIRO; et al. Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies. Carbohydrate Polymers, v. 299, p. 12-pg., 2023-01-01. (19/10942-0, 15/13684-0)

VELDMAN, WAYDE; LIBERATO, MARCELO VIZONA; ALMEIDA, VITOR M.; SOUZA, VALQUIRIA P.; FRUTUOSO, MAIRA A.; MARANA, SANDRO R.; MOSES, VUYANI; BISHOP, OZLEM TASTAN; POLIKARPOV, IGOR. X-ray Structure, Bioinformatics Analysis, and Substrate Specificity of a 6-Phospho-β-glucosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 60, n. 12, p. 16-pg., 2020-12-28. (15/13684-0)

VIEIRA, F. R.; PECCHIA, J. A.; SEGATO, F.; POLIKARPOV, I.. Exploring oyster mushroom (Pleurotus ostreatus) substrate preparation by varying phase I composting time: changes in bacterial communities and physicochemical composition of biomass impacting mushroom yields. Journal of Applied Microbiology, v. 126, n. 3, p. 931-944, MAR 2019. (13/21545-5, 14/18714-2, 15/13684-0, 09/52840-7)

BERNARDES, A.; PELLEGRINI, V. O. A.; CURTOLO, F.; CAMILO, C. M.; MELLO, B. L.; JOHNS, M. A.; SCOTT, J. L.; GUIMARAES, F. E. C.; POLIKARPOV, I.. Carbohydrate binding modules enhance cellulose enzymatic hydrolysis by increasing access of cellulases to the substrate. Carbohydrate Polymers, v. 211, p. 57-68, MAY 1 2019. (10/18773-8, 15/13684-0)

NETO, MARIO DE OLIVEIRA; FERNANDES, ADRIANO DE FREITAS; PIIADOV, VASSILI; CRAIEVICH, ALDO FELIX; DE ARAUJO, EVANDRO ARES; POLIKARPOV, IGOR. SAXSMoW 3.0: New advances in the determination of the molecular weight of proteins in dilute solutions from SAXS intensity data on a relative scale. Protein Science, v. 31, n. 1, SI, NOV 2021. (18/22300-0, 15/13684-0)

HIGASI, PAULA M. R.; VELASCO, JOSMAN A.; PELLEGRINI, VANESSA O. A.; DE ARAUJO, EVANDRO A.; FRANCA, BRUNO ALVES; KELLER, MALENE B.; LABATE, CARLOS A.; BLOSSOM, BENEDIKT M.; SEGATO, FERNANDO; POLIKARPOV, IGOR. Light-stimulated T. thermophilus two-domain LPMO9H: Low-resolution SAXS model and synergy with cellulases. Carbohydrate Polymers, v. 260, MAY 15 2021. (15/13684-0, 17/01165-4)

SONODA, MILTON T.; GODOY, ANDRE S.; PELLEGRINI, VANESSA O. A.; KADOWAKI, MARCO A. S.; NASCIMENTO, ALESSANDRO S.; POLIKARPOV, IGOR. Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1863, n. 6, p. 1015-1026, JUN 2019. (14/06565-2, 15/13684-0, 17/18173-0)

SEIKI KADOWAKI, MARCO ANTONIO; DE GODOY, MARIANA ORTIZ; KUMAGAI, PATRICIA SUEMY; DA COSTA-FILHO, ANTONIO JOSE; MORT, ANDREW; PRADE, ROLF ALEXANDER; POLIKARPOV, IGOR. Characterization of a New Glyoxal Oxidase from the Thermophilic Fungus Myceliophthora thermophila M77: Hydrogen Peroxide Production Retained in 5-Hydroxymethylfurfural Oxidation. CATALYSTS, v. 8, n. 10, OCT 2018. (15/13684-0, 11/20505-4)

BARRA, ANGELICA LUANA C.; DANTAS, LIVIA DE OLIVEIRA C.; MORAO, LUANA GALVAO; GUTIERREZ, RAISSA F.; POLIKARPOV, IGOR; WRENGER, CARSTEN; NASCIMENTO, ALESSANDRO S.. Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance. FRONTIERS IN PUBLIC HEALTH, v. 8, FEB 28 2020. (15/13684-0, 17/18173-0, 15/26722-8)

SEPULCHRO, ANA GABRIELA VEIGA; PELLEGRINI, VANESSA O. A.; BRIGANTI, LORENZO; DE ARAUJO, EVANDRO A.; DE ARAUJO, SIMARA S.; POLIKARPOV, IGOR. Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase. Carbohydrate Polymers, v. 247, NOV 1 2020. (15/13684-0)