Fish oil prevents changes induced by a high-fat diet on metabolism and adipokine secretion in mice subcutaneous and visceral adipocytes

Autor(es)

da Cunha de Sa, Roberta D. C. ; Crisma, Amanda R. ; Cruz, Maysa M. ; Martins, Amanda R. ; Masi, Laureane N. ; do Amaral, Catia L. ; Curi, R. ; Alonso-Vale, Maria I. C.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYSIOLOGY-LONDON; v. 594, n. 21, p. 6301-6317, NOV 2016.
• Citações Web of Science: 16

Resumo

In the present study, we investigated the effect of fish oil (FO) on metabolism and adipokine production by adipocytes from s.c. (inguinal; ING) and visceral (retroperitoneal; RP) white adipose depots in high-fat (HF) diet-induced obese mice. Mice were divided into CO (control diet), CO+FO, HF and HF+FO groups. The HF group presented higher body weight, glucose intolerance, insulin resistance, higher plasma total and low-density lipoprotein cholesterol levels, and greater weights of ING and RP adipose depots accompanied by hypertrophy of the adipocytes. FO exerted anti-obesogenic effects associated with beneficial effects on dyslipidaemia and insulin resistance in mice fed a HF diet (HF+FO group). HF raised RP adipocyte lipolysis and the production of pro-inflammatory cytokines and reduced de novo synthesis of fatty acids, whereas, in ING adipocytes, it decreased glucose uptake and adiponectin secretion but did not change lipolysis. Therefore, the adipose depots play different roles in HF diet-induced insulin resistance according to their location in the body. Concerning cytokine secretion, adipocytes per se in addition to white adopise tissue infiltrated leukocytes have to be considered in the aetiology of the comorbidities associated with obesity. Evidence is presented showing that previous and concomitant administration of FO can prevent changes in metabolism and the secretion of hormones and cytokines in ING and RP adipocytes induced by HF. (AU)

• Processo FAPESP: 11/51627-8 - Efeito de ácidos graxos sobre a diferenciação e função do tecido adiposo
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/07596-8 - Avaliação das alterações de Metilação do DNA na obesidade induzida por dieta hiperlipídica e o possível efeito modulador dos ácidos graxos ômega-3 in vivo
• Beneficiário: Cátia Lira Do Amaral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/05669-8 - Efeito da suplementação com ácidos graxos poliinsaturados ômega-3 e do exercício físico regular sobre a resistência à insulina induzida por dieta hiperlipídica
• Beneficiário: Amanda Roque Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 13/03268-4 - Atividades metabólicas e endócrinas de adipócitos extraídos do tecido adiposo sucutâneo e visceral de animais obesos induzidos por dieta hiperlipídica e suplementados com ácidos graxos ômega-3
• Beneficiário: Roberta Dourado Cavalcante da Cunha de Sá
• Modalidade de apoio: Bolsas no Brasil - Mestrado