Concentration of bioactive compounds from grape marc using pressurized liquid extraction followed by integrated membrane processes

Autor(es)

Vitor Pereira, Debora Tamires ^[1] ; Marson, Gabriela Vollet ^{[1, 2]} ; Fernandez Barbero, Gerardo ^[3] ; Tarone, Adriana Gadioli ^[1] ; Betim Cazarin, Cinthia Bau ^[1] ; Hubinger, Miriam Dupas ^[1] ; Martinez, Julian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, UNICAMP, BR-13083862 Campinas, SP - Brazil

^[2] Univ Montpellier, Inst Europeen Membranes, ENSCM, CNRS, UM, CC 047, 2 Pl Eugene Bataillon, F-34095 Montpellier - France

^[3] Univ Cadiz, Fac Sci, Dept Analyt Chem, CeiA3, IVAGRO, Republ Saharaui Ave S-N, Cadiz 11510 - Spain

• Tipo de documento: Artigo Científico
• Fonte: Separation and Purification Technology; v. 250, NOV 1 2020.
• Citações Web of Science: 0

Resumo

The integration of pressurized liquid extraction (PLE) with membrane separation technology was used to recover and concentrate monomeric anthocyanins and total phenolics from grape marc. The extracts obtained by PLE were subjected to a preliminary study of stirred dead-end filtration using different commercial flat sheet membranes of nanofiltration (NF). In addition, to reduce membrane fouling and the content of solids in the feed, previous microfiltration (MF) and ultrafiltration (UF) processes were evaluated. The membranes that presented the best performances were selected for cross-flow filtration. The flux of permeate, the retention of bioactive compounds and antioxidant capacity, and the characterization of the membrane surfaces were used to evaluate the performance of the filtration processes. The results obtained in this study suggest that the sequential process based on the previous MF with the MV020 membrane of the grape extract obtained by PLE, followed by the NF step with the NP010 membrane, was the most effective in the concentration of bioactive compounds. The sequential process MV020-NP010 provided excellent results in terms of permeate flux, concentration factor (2.4), retention coefficients of monomeric anthocyanins (78.2%) and total phenolics (71%), and high antioxidant capacity (52%) compared to other tested NF membranes. Fifteen anthocyanins were concentrated in the extracts by UHPLC-UV-Vis. The NP010 membrane had the smallest change in membrane roughness and functional groups after NF. It is concluded that the integration of PLE with the projected sequential process (MV020-NP010) is efficient for the recovery and concentration of bioactive compounds from grape marc, being promising for obtaining functional products with high added value. (AU)

• Processo FAPESP: 18/04067-6 - Concentração e fracionamento por membranas de hidrolisado proteico de levedura residual cervejeira
• Beneficiário: Gabriela Vollet Marson
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 18/15737-2 - Biorrefinaria da casca do maracujá (Passiflora edulis sp.): extração de pectina e compostos bioativos e concentração dos extratos por membranas
• Beneficiário: Débora Tamires Vitor Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/50593-2 - Concentração de extratos aquosos de própolis por nanofiltração
• Beneficiário: Miriam Dupas Hubinger
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/23670-2 - Biorrefinaria para aproveitamento de subprodutos do processamento de maracujá com tecnologias de alta pressão
• Beneficiário: Julian Martínez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/18465-8 - Concentração e fracionamento por membranas de hidrolisado protéico de levedura residual cervejeira
• Beneficiário: Gabriela Vollet Marson
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto