Enzymatic modification of arabinoxylans from soft and hard Argentinian wheat inhibits the viability of HCT-116 cells

Autor(es)

Paesani, Candela ^[1] ; Degano, Alicia L. ^[2] ; Ines Zalosnik, Maria ^[2] ; Fabi, Joao Paulo ^{[3, 4, 5]} ; Perez, Gabriela T. ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] CONICET UNC, ICYTAC Inst Ciencia & Tecnol Cordoba, Cordoba - Argentina

^[2] CONICET UNC, Dept Quim Biol Ranwel Caputto, CIQUIBIC Ctr Invest Quim Biol Cordoba, Cordoba - Argentina

^[3] Univ Sao Paulo, Food & Nutr Res Ctr NAPAN, Sao Paulo, SP - Brazil

^[4] Sao Paulo Res Fdn, Food Res Ctr FoRC, CEPID FAPESP Res Innovat & Disseminat Ctr, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, SP - Brazil

^[6] UNC, Fac Ciencias Agr, Catedra Quim Biol, Cordoba - Argentina

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 147, SEP 2021.
• Citações Web of Science: 1

Resumo

Dietary fiber plays an important role in the prevention of colorectal cancer, and arabinoxylans are an important source of this in grains, with some studies reporting the inhibition of cancer cell growth. However, very few studies have been conducted on this, and most previous studies have used oligosaccharides derived from arabinoxylans of specific molecular weight. The aim of this work is to extract, isolate, and analyze arabinoxylans from two different Argentinian genotypes of wheat (hard and soft) and study if they have the capacity to decrease the cellular viability of a colon cancer line (HCT-116). To determine whether the molecular size influences the inhibition of HCT-116 cell viability, specific hydrolysis was performed with endoxylanase, and the cells were exposed to the hydrolyzed arabinoxylans. The arabinoxylans treatment resulted in HCT-116 cell viability of 74% for the soft genotype and 64% for the hard genotype in comparison to nontreated cells. Hydrolyzedarabinoxylans result in HCT-116 cell viability of 68% for soft and 36% for hard genotypes (the lowest IC50 values) compared to nontreated cells. More importantly, no decrease after the arabinoxylans treatment was observed in the viability of murine noncancer cells known to rapidly respond to polysaccharide presence. The arabinoxylans from hard wheat showed more disubstituted xylose and alpha-1,2/alpha-1,3 linkages than the arabinoxylans from soft wheat, the possible cause for showing the best in vitro biological effect. The results showed other beneficial effects than the prebiotic ones and support the use of enzymatic treatment to increase the biological impacts of arabinoxylans. (AU)

• Processo FAPESP: 19/11816-8 - Efeito das fibras alimentares modificadas de mamões papaia em co-cultura tridimensional de células humanas de câncer de cólon e em modelo in vivo de ratos com carcinogênese de cólon quimicamente induzida
• Beneficiário: Joao Paulo Fabi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/23970-2 - Alterações biológicas das pectinas de mamão com possíveis benefícios à saúde humana
• Beneficiário: Joao Paulo Fabi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores