Enzymatic modification of arabinoxylans from soft and hard Argentinian wheat inhibits the viability of HCT-116 cells

Author(s)

Paesani, Candela ^[1] ; Degano, Alicia L. ^[2] ; Ines Zalosnik, Maria ^[2] ; Fabi, Joao Paulo ^{[3, 4, 5]} ; Perez, Gabriela T. ^{[1, 6]}

Affiliation

^[1] CONICET UNC, ICYTAC Inst Ciencia & Tecnol Cordoba, Cordoba - Argentina

^[2] CONICET UNC, Dept Quim Biol Ranwel Caputto, CIQUIBIC Ctr Invest Quim Biol Cordoba, Cordoba - Argentina

^[3] Univ Sao Paulo, Food & Nutr Res Ctr NAPAN, Sao Paulo, SP - Brazil

^[4] Sao Paulo Res Fdn, Food Res Ctr FoRC, CEPID FAPESP Res Innovat & Disseminat Ctr, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, SP - Brazil

^[6] UNC, Fac Ciencias Agr, Catedra Quim Biol, Cordoba - Argentina

• Document type: Journal article
• Source: Food Research International; v. 147, SEP 2021.
• Web of Science Citations: 1

Abstract

Dietary fiber plays an important role in the prevention of colorectal cancer, and arabinoxylans are an important source of this in grains, with some studies reporting the inhibition of cancer cell growth. However, very few studies have been conducted on this, and most previous studies have used oligosaccharides derived from arabinoxylans of specific molecular weight. The aim of this work is to extract, isolate, and analyze arabinoxylans from two different Argentinian genotypes of wheat (hard and soft) and study if they have the capacity to decrease the cellular viability of a colon cancer line (HCT-116). To determine whether the molecular size influences the inhibition of HCT-116 cell viability, specific hydrolysis was performed with endoxylanase, and the cells were exposed to the hydrolyzed arabinoxylans. The arabinoxylans treatment resulted in HCT-116 cell viability of 74% for the soft genotype and 64% for the hard genotype in comparison to nontreated cells. Hydrolyzedarabinoxylans result in HCT-116 cell viability of 68% for soft and 36% for hard genotypes (the lowest IC50 values) compared to nontreated cells. More importantly, no decrease after the arabinoxylans treatment was observed in the viability of murine noncancer cells known to rapidly respond to polysaccharide presence. The arabinoxylans from hard wheat showed more disubstituted xylose and alpha-1,2/alpha-1,3 linkages than the arabinoxylans from soft wheat, the possible cause for showing the best in vitro biological effect. The results showed other beneficial effects than the prebiotic ones and support the use of enzymatic treatment to increase the biological impacts of arabinoxylans. (AU)

• FAPESP's process: 19/11816-8 - Effect of papaya modified fibers on three-dimensional co-culture of human colon cancer cells and on in vivo model of rats with chemically induced colon carcinogenesis
• Grantee: Joao Paulo Fabi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 12/23970-2 - Biological changes of papaya pectins with possible benefits to human health
• Grantee: Joao Paulo Fabi
• Support Opportunities: Research Grants - Young Investigators Grants