| Texto completo | |
| Autor(es): |
Mantovani, Raphaela A.
;
Xavier, Ana Augusta O.
;
Tavares, Guilherme M.
;
Mercadante, Adriana Z.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Food Research International; v. 161, p. 15-pg., 2022-08-27. |
| Resumo | |
Considering that carotenoids are found acylated to fatty acids in most edible fruits, the influence of the ratio of free to acylated lutein on the hydrolysis extent and bioaccessibility was evaluated by in vitro digestion. For this purpose, for the first time, esterified, free, or a mixture of both carotenoid forms was used in the lipid phase of emulsions stabilized by sodium caseinate (NaCas) and native phosphocaseinate (PPCN). Marigold petals was used as a source of lutein-rich extracts. The emulsions were characterized and the extent of ester hydrolysis, carotenoid recovery, and bioaccessibility were evaluated by LC-DAD-MS/MS. Besides low polydispersity, NaCas and PPCN stabilized emulsions exhibited a constant mean droplet diameter of about 260 and 330 nm, respectively, after 7 days. Caseins were completely digested after the gastric digestion step. Moreover, casein supramolecular structure did not significantly affect carotenoid bioaccessibility. Lutein was majorly found in its free form in all bioaccessible fractions. The carotenoid bioaccessibility increased from 3% to 40% by increasing the percentage of free carotenoids from 0.5 to 100% in the emulsions; but the carotenoid recovery and hydrolysis extent of lutein esters were not affected. In conclusion, emulsion-based systems for carotenoid delivery stabilized either by NaCas or PPCN provided similar carotenoid bioaccessibility. Furthermore, bioaccessibility was inversely dependent on the overall hydrophobicity of the carotenoid extract. Our results suggest that the low bioaccessibility of esterified carotenoids was a consequence of their limited hydrolysis extent. This study provides information that may help design emulsion-based systems stabilized by food protein as a vehicle for carotenoids. (AU) | |
| Processo FAPESP: | 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais |
| Beneficiário: | Eneida de Paula |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/09214-4 - Como modificações estruturais de proteínas lácteas afetam a funcionalidade de compostos bioativos em matrizes alimentares complexas? |
| Beneficiário: | Guilherme Miranda Tavares |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 17/26237-8 - Entendendo como a interação proteína-carotenoide influência a estabilidade de emulsões e a bioacessibilidade de carotenóides |
| Beneficiário: | Raphaela de Araujo Mantovani |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos |
| Beneficiário: | Bernadette Dora Gombossy de Melo Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 18/23752-1 - EMU concedido no processo 2013/07914-8 sistema de cromatografia líquida de alta velocidade acoplado a detectores de arranjo de diodos, fluorescência e massas |
| Beneficiário: | Adriana Zerlotti Mercadante |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |