Apoptosis in tumors

• Grant number: 04/09932-4
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2005
• End date: May 31, 2009
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Marcello Fabiano de Franco
• Grantee: Marcello Fabiano de Franco
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: José Antonio Silva Junior ; Luciano Neder Serafini

Abstract

Apoptosis is the mechanism of cell death which promotes the removal of cells in normal tissue and is fundamental during development. In tumors, it can be the result of extra cellular stimulus or of characteristics intrinsic to the cells, depending on the expression of oncogenes. Alterations in genes which codify endonuclear caspases, receptors of cell death and mitochondrial proteins are observed frequently in tumors and in infiltrating leucocytes. These alterations result in structural changes determined by several molecular interactions depending on the stimulus path mobilized. This project aims to analyze the various paths involved in apoptosis in three neoplasms derived from the endodermic (gastric and colorectal tumors) and neurorectodermic (glioblastomas) tissue layers, collected by the FAPESP-Clinical Genome project. We will quantify cells in apoptosis and will evaluate the genetic and proteic expression of related molecules with the intrinsic path: p53, pRb, caspases 3, bcl-2, Bad, Bak, Bax, PTEN (phosphatase and tensin homologue deleted from chromosome 10) and extrinsic of apoptosis: Faz, FasL, caspase 8, ILK (integrin-linked kinase), growth factors (NGF - nerve growth factor, EGF - epidermal growth factor, FGF - fibroblast growth factor). This data will be correlated with histopathological and clinical parameters of the tumors: degree of differentiation staging and evolution (data derived from the FAPESP-Clinical Genome project) and in the future integrated to the genetic study of tumors. The project will make it possible to add information between the three central parameters of molecular biology (DNA-RNA-protein) and the study of specific populations from different regions of the state of São Paulo. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, THIAGO S.; OSHIMA, CELINA T. F.; SEGRETO, HELENA R. C.; BARRAZUETA, LUIS M.; COSTA, HENRIQUE O.; LIMA, FLAVIO O.; FORONES, NORA M.; RIBEIRO, DANIEL A.. The extrinsic apoptotic signaling pathway in gastric adenocarcinomas assessed by tissue microarray. PATHOLOGY RESEARCH AND PRACTICE, v. 207, n. 10, p. 613-617, 2011. (04/09932-4)

MESIAS BARREZUETA, LUIS FERNANDO; FUJIYAMA OSHIMA, CELINA TIZUKO; LIMA, FLAVIO OLIVEIRA; COSTA, HENRIQUE DE OLIVEIRA; GOMES, THIAGO SIMAO; ARTIGIANI NETO, RICARDO; DE RANCO, MARCELLO FABIANO. The intrinsic apoptotic signaling pathway in gastric adenocarcinomas of Brazilian patients: Immunoexpression of the Bcl-2 family (Bcl-2, Bcl-x, Bak, Bax, Bad) determined by tissue microarray analysis. MOLECULAR MEDICINE REPORTS, v. 3, n. 2, p. 261-267, MAR-APR 2010. (04/09932-4)

STEFANO, JOSE T.; DE OLIVEIRA, CLAUDIA P. M. S.; CORREA-GIANNELLA, MARIA L.; SOARES, IBERE C.; KUBRUSLY, MARCIA S.; BELLODI-PRIVATO, MARTA; DE MELLO, EVANDRO S.; DE LIMA, VICENCIA M. R.; CARRILHO, FLAIR J.; ALVES, VENANCIO A. F.. Decreased immunoexpression of survivin could be a potential marker in human non-alcoholic fatty liver disease progression?. LIVER INTERNATIONAL, v. 31, n. 3, p. 377-385, MAR 2011. (04/09932-4)

SAGGIORO, FABIANO P.; NEDER, LUCIANO; STAVALE, JOAO NORBERTO; PAIXAO-BECKER, ALINE NAZARETH P.; MALHEIROS, SUZANA M. F.; SOARES, FERNANDO A.; PITTELLA, JOSE EYMARD H.; MATIAS, CAIO CESAR M. S.; COLLI, BENEDICTO O.; CARLOTTI, JR., CARLOS GILBERTO; et al. Fas, FasL, and cleaved caspases 8 and 3 in glioblastomas: A tissue microarray-based study. PATHOLOGY RESEARCH AND PRACTICE, v. 210, n. 5, p. 267-273, 2014. (04/09932-4)

LIMA, FLAVIO DE OLIVEIRA; COSTA, HENRIQUE DE OLIVEIRA; MESIAS BARREZUETA, LUIS FERNANDO; FUJIYAMA OSHIMA, CELINA TIZUKO; SILVA, JR., JOSE ANTONIO; GOMES, THIAGO SIMAO; PINHEIRO, JR., NATHANAEL; ARTIGIANI NETO, RICARDO; FRANCO, MARCELLO. Immunoexpression of inhibitors of apoptosis proteins and their antagonist SMAC/DIABLO in colorectal carcinoma: Correlation with apoptotic index, cellular proliferation and prognosis. ONCOLOGY REPORTS, v. 22, n. 2, p. 295-303, AUG 2009. (04/09932-4)