Genetic and molecular basis of chlorophyll in seeds: a step forward to improve soybean adaptability to climate change

• Grant number: 17/50211-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: May 01, 2018
• End date: April 30, 2023
• Field of knowledge: Biological Sciences - Biology
• Agreement: Netherlands Organisation for Scientific Research (NWO)
• Principal Investigator: Edvaldo Aparecido Amaral da Silva
• Grantee: Edvaldo Aparecido Amaral da Silva
• Principal researcher abroad: Henk Wilhelmus Maria Hilhorst
• Institution abroad: Wageningen University, Netherlands
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 22/11417-9 - Identification of SNP (Single Nucleotide Polymorphisms) markers in genomic regions associated with the regulation of chlorophyll degradation in soybean seeds, BP.PD; 21/03209-4 - How are the transcription factors ABI3 and ABI5 associated with chlorophyll retention in soybean seeds?, BP.IC; 20/12405-9 - Genetic and molecular basis of chlorophyll retention in seeds: a step forward to improve soybean adaptability to climate change, BP.PD; 19/06160-6 - Influence of the environment on the occurrence of green seeds, production of oil and protein in a soybean genotype with accumulation of ß-Carotene, BP.DR

Abstract

Due to global climate change, agricultural areas in the world are increasingly prone to climatic extremes. For most species, extreme temperatures and drought stress affects seed quality. This is particularly problematic in oilseeds, such as soybean and canola, and often results in what is known as the "green seed problem". This is when chlorophyll is retained in ripening seeds, resulting in lower oil and seed quality. Despite its considerable importance for seed quality and food security, the mechanisms underlying chlorophyll degradation in oilseeds are poorly understood, which is a major limiting facto r in research into applied solutions to the green seed problem. Over the past years, our groups have accumulated fundamental and applied knowledge on chlorophyll retention in seeds and a solid and fruitful collaboration between Wageningen University and Research and Brazilian research institutes Universidade Estadual Paulista (UNESP) and Embrapa-Soja, both responsible for the transfer of fundamental knowledge into crops. Now we aim to use this knowledge to identify the main regulators of chlorophyll degradation in seeds, to understand the biological significance of chlorophyll in seeds and identify loci responsible for chlorophyll degradation in soybean seeds. For this, we propose to use a combination of physiological, genetic and molecular approaches using a model plant to shed Iight on the molecular regulatory networks of chlorophyll degradation in seeds, concomitantly with a GBS-GWAS approach that will be used in soybean to allow the association between fundamental and applied research. The identification of genetic and molecular markers will greatly aid plant breeders in selecting varieties with desired oil, protein and seed quality traits, together with a low susceptibility to green seed formation. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATISTA, THIAGO BARBOSA; PERISSATO, SAMARA MOREIRA; QUEIROZ REGO, CARLOS HENRIQUE; FONSECA DE OLIVEIRA, GUSTAVO ROBERTO; HENNING, FERNANDO AUGUSTO; AMARAL DA SILVA, EDVALDO APARECIDO. Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?. JOURNAL OF SEED SCIENCE, v. 43, 2021. (17/50211-9, 19/06160-6)

DA SILVA, TIAGO ALEXANDRE; COSTA CRUSCIOL, CARLOS ALEXANDRE; BATISTA, THIAGO BARBOSA; BOSSOLANI, JOAO WILLIAM; FONSECA DE OLIVEIRA, GUSTAVO ROBERTO; BASSO, DENISE PUNTEL; DE ALMEIDA CARMEIS FILHO, ANTONIO CARLOS; BRAVO, JULIANA PEREIRA; AMARAL DA SILVA, EDVALDO APARECIDO. Liming enhances longevity of wheat seeds produced in acid soils. SCIENTIFIC REPORTS, v. 12, n. 1, p. 10-pg., 2022-10-27. (14/16712-2, 17/50211-9, 13/18462-0)

RIBEIRO-OLIVEIRA, JOAO PAULO; SILVEIRA, LILIAN E. D.; PINTO, LILIAN V. A.; SILVA, EDVALDO A. A.; HILHORST, HENK W. M.. Clues on an intraspecific communication system in seed-seedling transition. PHYSIOLOGIA PLANTARUM, v. 172, n. 3, p. 1609-1618, JUL 2021. (17/50211-9, 20/12405-9)

DE ALBUQUERQUE, JANAI PEREIRA; RIBEIRO-OLIVEIRA, JOAO PAULO; DE OLIVEIRA-JUNIOR, JOAO BOSCO; COSTA, FREDERICO HENRIQUE DA SILVA; DA SILVA, EDVALDO APARECIDO AMARAL. Understanding In Vitro Embryo Development through Classical Germination Measurements: A Case Study of Dragon's Blood (Croton lechleri Mull Arg.). AGRICULTURE-BASEL, v. 13, n. 8, p. 13-pg., 2023-08-01. (17/50211-9)

CANTAO, RENATO FERNANDES; RIBEIRO-OLIVEIRA, JOAO PAULO; DA SILVA, EDVALDO A. AMARAL; DOS SANTOS, AMANDA RITHIELI; DE FARIA, RUTE QUELVIA; SARTORI, MARIA MARCIA PEREIRA. POMONA: a multiplatform software for modeling seed physiology. FRONTIERS IN PLANT SCIENCE, v. 14, p. 13-pg., 2023-07-06. (18/25698-4, 17/50211-9, 16/13126-0)

CHAMMA, LARISSA; DA SILVA, GUSTAVO FERREIRA; PERISSATO, SAMARA MOREIRA; ALIEVI, CLEONEI; CHAVES, PRINSCILLA PAMELA NUNES; GIANDONI, VALERIA CRISTINA RETAMEIRO; CALONEGO, JULIANO CARLOS; DA SILVA, EDVALDO APARECIDO AMARAL. Does Forced Plant Maturation by Applying Herbicide with Desiccant Action Influence Seed Longevity in Soybean?. PLANTS-BASEL, v. 12, n. 15, p. 12-pg., 2023-08-01. (17/50211-9)

ZINSMEISTER, JULIA; BERRIRI, SOUHA; BASSO, DENISE PUNTEL; LY-VU, BENOIT; DANG, THI-THU; LALANNE, DAVID; AMARAL DA SILVA, EDVALDO APARECIDO; LEPRINCE, OLIVIER; BUITINK, JULIA. The seed-specific heat shock factorA9regulates the depth of dormancy inMedicago truncatulaseeds viaABAsignalling. PLANT CELL AND ENVIRONMENT, v. 43, n. 10, AUG 2020. (17/50211-9)

DA SILVA, GUSTAVO FERREIRA; CHAMMA, LARISSA; LUPERINI, BRUNO CESAR OTTOBONI; CHAVES, PRINSCILLA PAMELA NUNES; CALONEGO, JULIANO CARLOS; NAKAGAWA, JOAO; DA SILVA, EDVALDO APARECIDO AMARAL. Physiological Quality of Soybean Seeds as a Function of Soil Management Systems and Pre-Harvest Desiccation. AGRONOMY-BASEL, v. 13, n. 3, p. 12-pg., 2023-03-01. (17/50211-9)

AJALA-LUCCAS, DAIANI; RIBEIRO-OLIVEIRA, JOAO PAULO; TEIXEIRA, RENAKE N.; DUCATTI, KARINA RENOSTRO; FRANCA-NETO, J. B.; HILHORST, HENK W. M.; DA SILVA, EDVALDO APARECIDO AMARAL. The Seed-Seedling Transition in Commercial Soybean Cultivars with the Presence of Greenish Seeds in the Sample: A Perspective from Classical Genetic Parameters. AGRONOMY-BASEL, v. 13, n. 8, p. 20-pg., 2023-08-01. (20/12405-9, 17/50211-9)

DUCATTI, KARINA RENOSTRO; BATISTA, THIAGO BARBOSA; HIRAI, WELINTON YOSHIO; LUCCAS, DAIANI AJALA; MORENO, LETICIA DE AGUILA; GUIMARAES, CRISTIANE CARVALHO; BASSEL, GEORGE W.; AMARAL DA SILVA, EDVALDO APARECIDO. Transcripts Expressed during Germination Sensu Stricto Are Associated with Vigor in Soybean Seeds. PLANTS-BASEL, v. 11, n. 10, p. 15-pg., 2022-05-01. (17/50211-9, 19/06160-6)