Development of portable biosensor for non-invasive detection of uric acid as indicative of hyperuricemia

• Grant number: 17/19470-8
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: August 31, 2020
• Field of knowledge: Engineering - Materials and Metallurgical Engineering
• Principal Investigator: Adriana Pavinatto da Costa
• Grantee: Adriana Pavinatto da Costa
• Host Institution: Universidade Brasil. Campus São Paulo. São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Bruno Vinícius Manzolli Rodrigues ; Daniel Souza Corrêa ; Mardoqueu Martins da Costa ; Mariana Amorim Fraga ; Vilson Rosa de Almeida
• Associated scholarship(s): 19/04279-6 - Production and characterization of nanostructured sensorial platforms for biosensors, BP.TT

Abstract

High concentrations of uric acid in the blood (hyperuricemia) carries to serious health problems such as gastrointestinal disorders, hypertension, cardiovascular and renal diseases, and gouty arthritis. Currently the diagnosis of uric acid concentration found in the blood is done through laboratory examination, being invasive and time-consuming analysis. In this context, the main objective of this project is the development of a portable electrochemical biosensor for the analysis of uric acid concentration in saliva for rapid and noninvasive diagnosis. Therefore, disposable printed electrodes should be used in the construction of a sensorial platform for the uricase enzyme immobilization and uric acid electrochemical detection. The platform should be composed with polymeric nanomaterials, metal nanoparticles or carbono-based nanoparticles as electrochemical signal amplifiers. After biosensor optimization, a prototype should be developed for electrochemical signal acquisition and correlation with uric acid concentration in saliva samples according to the calibration curve. The prototype should be able to display the analyte concentration through the digital monitor. Furthermore, It is hoped to assist in establishing correlation between the uric acid level found in saliva and blood of people with hyperuricemia and population in general. This Project aims to start the researches activities of the proponent in the Biomedical Engineering Postgraduate Program from University Brazil (Itaquera Campus) where she is recently full Professor. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; FACURE, MURILO H. M.; PENA, RAFAELLA B.; SANFELICE, RAFAELA C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Ultrasensitive biosensor based on polyvinylpyrrolidone/chitosan/reduced graphene oxide electrospun nanofibers for 17 alpha - Ethinylestradiol electrochemical detection. Applied Surface Science, v. 458, p. 431-437, NOV 15 2018. (17/19470-8, 17/12174-4, 13/26712-7)

PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; FACURE, MURILO H. M.; PENA, RAFAELLA B.; SANFELICE, RAFAELA C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Ultrasensitive biosensor based on polyvinylpyrrolidone/chitosan/reduced graphene oxide electrospun nanofibers for 17α - Ethinylestradiol electrochemical detection. Applied Surface Science, v. 458, p. 7-pg., 2018-11-15. (17/19470-8, 17/12174-4, 13/26712-7)

MATOS, M. D. L. P.; SANTOS, E. N.; SILVA, M. D. C. R. M.; PAVINATTO, A.; COSTA, M. M.; BASTOS-FILHO, TF; CALDEIRA, EMD; FRIZERA-NETO, A. Use of Fluorescence in the Diagnosis of Oral Health in Adult Patients Admitted to the Intensive Care Unit of a Public Emergency Hospital. XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020, v. N/A, p. 7-pg., 2022-01-01. (17/19470-8, 18/22628-5, 19/04279-6)

LEAL, SEANIA SANTOS; GUSMAO, GUSTAVO OLIVEIRA DE MEIRA; UCHOA, VALDILEIA TEIXEIRA; FIGUEIREDO-SILVA, JOSE; PINTO, LUCIELMA SALMITO SOARES; TIM, CARLA R.; ASSIS, LIVIA; MAIA-FILHO, ANTONIO LUIZ MARTINS; OLIVEIRA, RAUIRYS ALENCAR DE; LOBO, ANDERSON OLIVEIRA; et al. Evaluation of How Methacrylate Gelatin Hydrogel Loaded with Ximenia americana L. Extract (Steam Bark) Effects Bone Repair Activity Using Rats as Models. JOURNAL OF FUNCTIONAL BIOMATERIALS, v. 14, n. 9, p. 17-pg., 2023-09-01. (17/19470-8)

PACHECO, KAROLINE M. L.; TORRES, BRUNO B. M.; SANFELICE, RAFAELA C.; DA COSTA, MARDOQUEU M.; ASSIS, LIVIA; MARQUES, ROSEMARIE BRANDIM; MAIA FILHO, ANTONIO LUIZ MARTINS; TIM, CARLA R.; PAVINATTO, ADRIANA. Chitosan and chitosan/turmeric-based membranes for wound healing: Production, characterization and application. International Journal of Biological Macromolecules, v. 253, p. 9-pg., 2023-10-20. (17/19470-8)

CARONI, JOICIARA GARCIA; DE ALMEIDA MATTOS, ALEXIA VICTORIA; FERNANDES, KELLY ROSSETTI; BALOGH, DEBORA TEREZIA; MUNIZ RENNO, ANA CLAUDIA; OKURA, MONICA HITOMI; GRANATO MALPASS, ANA CLAUDIA; FERRARESI, CLEBER; GARCIA, LIVIA ASSIS; SANFELICE, RAFAELA CRISTINA; et al. Chitosan-based glycerol-plasticized membranes: bactericidal and fibroblast cellular growth properties. POLYMER BULLETIN, v. 78, n. 8, AUG 2020. (17/19470-8)

PAVINATTO, ADRIANA; DE ALMEIDA MATTOS, ALEXIA VICTORIA; GRANATO MALPASS, ANA CLAUDIA; OKURA, MONICA HITOMI; BALOGH, DEBORA TERESIA; SANFELICE, RAFAELA CRISTINA. Coating with chitosan-based edible films for mechanical/biological protection of strawberries. International Journal of Biological Macromolecules, v. 151, p. 1004-1011, MAY 15 2020. (17/19470-8)

LUIZA A. MERCANTE; RAFAELA S. ANDRE; JULIANA B. MACEDO; ADRIANA PAVINATTO; DANIEL S. CORREA. Nanofibras eletrofiadas e suas aplicações: avanços na última década. Química Nova, v. 44, n. 6, p. 717-736, 2021-08-11. (17/19470-8, 16/23793-4, 18/22214-6, 17/12174-4)

JULIANA B. MACEDO; RAFAELA C. SANFELICE; LUIZA A. MERCANTE; DANILO MARTINS DOS SANTOS; FILIPE HABITZREUTER; SÉRGIO PAULO CAMPANA-FILHO; ADRIANA PAVINATTO. ATIVIDADE ANTIMICROBIANA DE QUITOSANAS E SEUS DERIVADOS: INFLUÊNCIA DAS CARACTERÍSTICAS ESTRUTURAIS. Química Nova, v. 45, n. 6, p. 690-704, 2022-08-29. (17/20973-4, 17/19470-8)

DA SILVA, BRUNO BATISTA; MENEZES, JORGE EDUARDO; DA COSTA, MARDOQUEU MARTINS; DOS SANTOS, LAURITA; SANFELICE, RAFAELA CRISTINA; PAVINATTO, ADRIANA. Comparison of the acyclovir release carried in chitosan-based microstructured systems. POLYMER BULLETIN, v. N/A, p. 15-pg., 2023-08-11. (17/19470-8)

TEODORO, KELCILENE B. R.; SANFELICE, RAFAELA C.; MIGLIORINI, FERNANDA L.; PAVINATTO, ADRIANA; FACURE, MURILO H. M.; CORREA, DANIEL S.. A Review on the Role and Performance of Cellulose Nanomaterials in Sensors. ACS SENSORS, v. 6, n. 7, p. 2473-2496, JUL 23 2021. (18/22214-6, 17/21791-7, 17/19470-8, 17/12174-4, 17/10582-8)