Multi - user equipment approved in grant 2012/50191-4: mass spectrometer impact II - Bruker

• Grant number: 18/13588-0
• Support Opportunities: Multi-user Equipment Program
• Start date: August 01, 2018
• End date: July 31, 2025
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Maria Aparecida Juliano
• Grantee: Maria Aparecida Juliano
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 12/50191-4 - Synthesis, kinetic studies and applications of substrates and inhibitors for proteolytic enzymes, AP.TEM
• EMU web page: http://www.posgrad.epm.br/multiequipa/
• Type of equipment: Caracterização de Materiais - Espectrometria - De massas
• Manufacturer: Bruker
• Model: Impact II

Abstract

We have been using mass spectrometry since 1994 as a control tool in the parallel synthesis of peptides established by us since 1990 and this experience has accredited us to use mass spectrometry during development of new projects, as well as in the complementary projects. For 03 years UNIFESP has been structuring the area of oncology, adding researchers from different areas and different institutions of the State of São Paulo, with whom we already had collaborations on account of the peptides and now we are able to contribute with mass spectrometry. We are currently involved in collaborations with Dr. André Vettore and Dr. Giselle Zanker, professors from UNIFESP-Campus Diadema in the identification of classes of proteases of head and neck tumor cells; of brain and leukemic cells using substrates and inhibitors. The next step will be the identification of the proteins present in the cells, as well as the absolute and relative quantification of these proteins. Other projects are being developed aiming at the use of this tool, mainly in the field of clinical oncology for various purposes, such as biomarkers, being used for differentiation of the plasma secret in peptidoma and identification of post-translational modifications of patients with gastric cancer. This project will be performed in collaboration with Dr. Nora Manouikian Forones, Professor of the EPM/UNIFESP, with emphasis on gastroenterology/cancerology. From this thematic project (# 12 / 50191-4), a Nano-HPLC (Thermo) was purchased to be attached to an automatic dispenser on MALDI-TO / TOF (Proteineer FC, Bruker) plates and a Workstation with programs (Peaks studio, Waterloo, Canada; Profile Analysis and Protein Scape, Bruker Daltonics, Bremen, Germany) for proteomic analyzes. However, this system does not allow carrying out the experiments exposed above. The objective of this request is to make possible the purchase of the mass spectrometer Impact II (Bruker), already quoted by the company responsible, from the financial resource available in the project without budget increase, since the company manufacturer of the equipment already made feasible the sale according to the available resource. The equipment will be multiuser, since there are already collaborations with several researchers in the areas of basic and clinical research of the Escola Paulista de Medicina, as well as researchers from UNIFESP Campus - Diadema / SP, who they are looking forward to exploring applications that this tool can provide. The nanoLC system coupled to mass spectrometer and softwares, can perform the identification of proteins (proteomics topdown and botton-up), sequencing and identification of novel compounds, peptidomic and metabolomic analyze, besides to perform additional operations, such as label-free quantification, since it is possible to evaluate the chromatogram and intensity / area of the precursor ions and all tools are compatible with date acquired. Demands in the area of metabolomics may also be used since our HPLCs are compatible with the system. Finally, it is important to emphasize that the infrastructure installed on account of this thematic project, already mentioned above and added with the acquisition of equipment will be part of an ample project of metabolic analysis in the rejection of kidneys transplanted by Hospital Rim, which 900 to 950 transplants/year, being the most active kidney transplant center in the world. In this project, we will use the mass spectrometry associated to Magnetic Nuclear Resonance (NMR) in metabolomics analysis whose equipment and infrastructure have just been installed in INFAR under the coordination of Dra. Helena Nader. In addition, Bruker company, also manufacturer of NMR, will provide technical and logistic support for this technological innovation of MS / RMN association. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO NASCIMENTO, EDILZA SILVA; ANAYA, KATYA; DE OLIVEIRA, JULIA MARIANO CAJU; DE LACERDA, JOSE THALLES JOCELINO GOMES; MILLER, MICHAEL EDWARD; DIAS, MERIELLEN; MENDES, MARIA ANITA; PALLONE, JULIANA DE AZEVEDO LIMA; ARNS, CLARICE WEIS; JULIANO, MARIA APARECIDA; et al. Identification of bioactive peptides released from in vitro gastrointestinal digestion of yam proteins (Dioscorea cayennensis). Food Research International, v. 143, MAY 2021. (12/50191-4, 18/13588-0)

FREIRE, MARJORIE C. L. C.; NOSKE, GABRIELA D.; BITENCOURT, NATALIA V.; SANCHES, PAULO R. S.; SANTOS-FILHO, NORIVAL A.; GAWRILJUK, VICTOR O.; DE SOUZA, EDUARDO P.; NOGUEIRA, VICTOR H. R.; DE GODOY, MARIANA O.; NAKAMURA, ALINE M.; et al. Non-Toxic Dimeric Peptides Derived from the Bothropstoxin-I Are Potent SARS-CoV-2 and Papain-like Protease Inhibitors. Molecules, v. 26, n. 16, AUG 2021. (18/17095-8, 20/04602-9, 13/07600-3, 20/05761-3, 20/12519-4, 18/25600-4, 16/19712-9, 16/13884-2, 18/13588-0)

TELES, C. M.; LAMMOGLIA, L. C.; JULIANO, M. A.; RUIZ, A. L. T. G.; CANDIDO, T. Z.; DE CARVALHO, J. E.; LIMA, C. S. P.; ABBEHAUSEN, C.. Novel anticancer Pd-II complexes: The effect of the conjugation of transferrin binding peptide and the nature of halogen coordinated on antitumor activity. Journal of Inorganic Biochemistry, v. 199, OCT 2019. (17/12719-0, 18/13588-0, 16/07729-4)

GRACIOSO, LOUISE HASE; PENA-BAHAMONDE, JANIRE; KAROLSKI, BRUNO; BORREGO, BRUNA BACARO; PERPETUO, ELEN AQUINO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; HASHIGUCHI, HIROKI; JULIANO, MARIA APARECIDA; ROBLES HERNANDEZ, FRANCISCO C.; RODRIGUES, DEBORA FRIGI. Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper. SCIENCE ADVANCES, v. 7, n. 17, APR 2021. (15/18089-3, 18/22790-7, 13/11020-2, 13/50218-2, 18/13588-0)

NETO, OSCAR BENTO SILVA; VALLADAO, RODRIGO; COELHO, GUILHERME RABELO; DIAS, RENATA; PIMENTA, DANIEL CARVALHO; LOPES, ADRIANA RIOS. Spiders' digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family. SCIENTIFIC REPORTS, v. 13, n. 1, p. 13-pg., 2023-01-29. (15/23745-7, 18/13588-0)

NISHIYAMA-JR, MILTON Y.; TASHIMA, ALEXANDRE K.; NISHIDUKA, ERIKA S.; ABREU, THIAGO F.; ABUKAWA, FERNANDA MIDORI; OLIVEIRA, URSULA C.; TARDIVO, CAIO E. O.; NASCIMENTO, SORAIA M.; MEISSNER, GABRIEL O.; CHAIM, OLGA M.; et al. Multiomics Profiling of Toxins in the Venom of the Amazonian Spider Acanthoscurria juruenicola. JOURNAL OF PROTEOME RESEARCH, v. 21, n. 11, p. 15-pg., 2022-10-19. (17/20106-9, 18/13588-0, 13/07467-1)

DE OLIVEIRA, TALITA MENDES; JOCELINO GOMES DE LACERDA, JOSE THALLES; FIGUEIREDO LEITE, GIUSEPPE GIANINI; DIAS, MERIELLEN; MENDES, MARIA ANITA; KASSAB, PAULO; SALES E SILV, CLISLEYD GLAUCIELLY; JULIANO, MARIA APARECIDA; FORONES, NORA MANOUKIAN. Label-free peptide quantification coupled with in silico mapping of proteases for identification of potential serum biomarkers in gastric adenocarcinoma patients. CLINICAL BIOCHEMISTRY, v. 79, p. 61-69, MAY 2020. (18/13588-0, 12/50191-4)

BEZERRA, TALIANA; ESTEVEZ, MARIO; LACERDA, JOSE THALLES; DIAS, MERIELLEN; JULIANO, MARIA; MENDES, MARIA ANITA; MORGANO, MARCELO; PACHECO, MARIA TERESA; MADRUGA, MARTA. Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS2 and Bioactivity Assessment. Molecules, v. 25, n. 7, APR 2020. (18/13588-0, 12/50191-4)

ALENCAR BEZERRA, TALIANA KENIA; JOCELINO GOMES DE LACERDA, JOSE THALLES; SALU, BRUNO RAMOS; VILELA OLIVA, MARIA LUIZA; JULIANO, MARIA APARECIDA; PACHECO, MARIA TERESA BERTOLDO; MADRUGA, MARTA SUELY. Identification of Angiotensin I-Converting Enzyme-Inhibitory and Anticoagulant Peptides from Enzymatic Hydrolysates of Chicken Combs and Wattles. JOURNAL OF MEDICINAL FOOD, v. 22, n. 12, DEC 2019. (18/13588-0, 12/50191-4)