A collaborative study between Brazilian and US scientists: the effects of environmental chemical exposures on reproduction

• Grant number: 18/50002-3
• Support type: Regular Research Grants
• Duration: August 01, 2018 - December 31, 2021
• Field of knowledge: Biological Sciences - Morphology
• Cooperation agreement: University of Illinois
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal researcher: Wellerson Rodrigo Scarano
• Grantee: Wellerson Rodrigo Scarano
• Principal researcher abroad: Jodi Anne Flaws
• Institution abroad: University of Illinois at Chicago (UIC), United States
• Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 17/08306-2 - Oncotoxicological programming by the material exposure to the mixture of different fatalatos: multigenerational effect on prostate of rats and gene and epigenetic aspects related to prostate adenocarcinoma, AP.R

Abstract

The incidence of chronic diseases such as diabetes and obesity, as well as genetic diseases such as cancer, has increased in recent decades due to several socio-economic and environmental factors. Thus, interest in environmental factors that interfere without intra-uterine development and rnorphofunctional, genetic and epigenetic promovamzy, including how they can be transmitted to offspring are of paramount importance. The literature has shown that some toxic medications have an ability to increase susceptibility to cancer, creating a scientific perspective of great interest to our group. Phthalates represent a group of chemicals in the industry and are found in different concentrations in the urine and without plasma of humans. Thus, the objective of the study will be to investigate whether a gestational and lactational exposure to different concentrations of a phthalate mixture of phthalates and capable of modifying the rat prostate morphology, as well as altering the expression of mRNAs and miRNAs related with tumorigenesis and development in the F1 and F2 generations. Pregnant female rats (DG10 to DPN21) will be divided into 4 experimental groups: control (vehicle); 20 ug /kg/day, 200 ug/kg/day, 200 mg/kg/day) and they will receive these concentrations in the following ratio: 19% DEHP, 36% DEP, 15% DBP, 10% DiBP, 8 % BBzP and 10% DiNP. The prostate will be collected for the analyzes in the DPN22 and 120 (F1) and I DPN22 (F2). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SCARANO, WELLERSON R.; BEDRAT, AMINA; ALONSO-COSTA, LUIZ G.; AQUINO, ARIANA M.; FANTINATTI, BRUNO E. A.; JUSTULIN, LUIS A.; BARBISAN, LUIS F.; FREIRE, PAULA P.; FLAWS, JODI A.; LEMOS, BERNARDO. Exposure to an Environmentally Relevant Phthalate Mixture During Prostate Development Induces MicroRNA Upregulation and Transcriptome Modulation in Rats. TOXICOLOGICAL SCIENCES, v. 171, n. 1, p. 84-97, SEP 2019. (17/22275-2, 17/08306-2, 18/50002-3)

DE FREITAS, THIAGO; ZAPATERINI, JOYCE R.; MOREIRA, CRISTIANE M.; DE AQUINO, ARIANA M.; ALONSO-COSTA, LUIZ G.; BIDINOTTO, LUCAS T.; KASS, LAURA; FLAWS, JODI A.; SCARANO, WELLERSON R.; BARBISAN, LUIS F.. Prenatal exposure to a mixture of different phthalates increases the risk of mammary carcinogenesis in F1 female offspring. Food and Chemical Toxicology, v. 156, OCT 2021. (17/08306-2, 18/50002-3)