Effects of diacerein, conjugate glucosamine/chondroitin and dental pulp stem cells on Wistar rats femur articular cartilage with osteoarthritis.

• Grant number: 18/11235-2
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2018
• End date: September 30, 2020
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Marcelo Cavenaghi Pereira da Silva
• Grantee: Marcelo Cavenaghi Pereira da Silva
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Adriana da Costa Neves ; Diogo Correa Maldonado

Abstract

Osteoarthritis is the main disease affecting joint cartilage and the most common joint disease in the world that causes major disability. The present study aims to evaluate different types of osteoarthritis treatments in the medial femoral condyle of Wistar rats. The animals will be treated with diacerein (50mg / kg), glucosamine (500mg / kg) / chondroitin conjugate (400mg / kg) and human deciduous dental pulp stem cells. Twenty-five male Wistar rats were distributed in 5 groups with five animals (n = 5): control group, negative group, diacerein-treated arthritis group, glycosamine/chondroitin conjugated arthritis group and deciduous dental pulp stem cells treated group. Arthritis will be induced by joint infiltration with zymosan (1mg) in the left knee of the animals. After 14 days, the animals will be treated for sixty continuous days with the drugs diacerein and glycosamine/chondroitin conjugate by gavage. The deciduous dental pulp stem cells group will receive single intra-articular application of 8x105 cells in 4mL of saline solution. After the sixty-day period, all animals will be euthanized using intraperitoneal injection with excess of Ketamine and Xylazine (180mg and 20 mg / kg body weight). The medial femoral condyles will be removed, fixed, decalcified for light microscopy protocol and immunohistochemistry. Morphometry, stereology and expression of Sox5, Sox6, Ihh, MMp8, MMp13 and Collagen 2 will be analyzed. The results will be expressed as means, medians and p values less than 0,05 will be considered significant after ANOVA and Tukey Post Hoc multiple comparison test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MALDONADO, D. CORREA; NICOLICHE, T.; FABER, J.; KERKIS, I; SAEZ, D. MARTINEZ; SASAKI, R. TETSUO; PEREIRA DA SILVA, M. CAVENAGHI. Intra-articular human deciduous dental pulp stem cell administration vs. pharmacological therapy in experimental osteoarthritis rat mode. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, v. 25, n. 9, p. 3546-3556, 2021. (18/11235-2)

NICOLICHE, TIAGO; MALDONADO, DIOGO CORREA; FABER, JEAN; PEREIRA DA SILVA, MARCELO CAVENAGHI. Evaluation of the articular cartilage in the knees of rats with induced arthritis treated with curcumin. PLoS One, v. 15, n. 3, MAR 12 2020. (18/11235-2)