Characterization of recombinant antigens and immune response of a new rabies vaccine candidate

• Grant number: 18/10538-1
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2018
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Renato Mancini Astray
• Grantee: Renato Mancini Astray
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Elisabeth de Fatima Pires Augusto ; Helio Langoni ; Soraia Attie Calil Jorge

Abstract

Rabies control is one of the global health challenges. While under developed countries are still implementing basic prevention activities against human and animal rabies, developed countries which have already controlled urban rabies are challenged for its control in sylvatic areas. Currently available vaccines are generally effective but present high production costs, biosafety risks and short term immune protection. The possibility of obtaining a recombinant vaccine against rabies has guided our studies for years. An effective vaccine preparation that combines high immune response mediated by neutralizing antibodies, with a good level of cellular immune response, can lead to the proposition of a new and more powerful anti-rabies vaccine. Several technologies may give rise to this preparation. The approaches considered in this project involve the production of the vaccine antigen, the rabies Lyssavirus glycoprotein (GPV), by S2 insect cells or in the VLP form through the baculovirus-Sf9 system. The production of GPV by these systems already has a good level of knowledge by our group. Our focus at this moment is the characterization of purified GPV according to biochemical aspects of composition and structure and according to immunological aspects, through the analysis of epitopes and the immune response in mice. The project also covers the study of the immune response in the context of the addition of modern adjuvants to vaccine preparations. As a result of this project, an animal vaccine candidate, based on adjuvant-associated recombinant protein, is expected to be proposed. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PILATTI, LIVIA; MANCINI ASTRAY, RENATO; ROCCA, MAYRA PEREIRA; BARBOSA, FLAVIA FERREIRA; JORGE, SORAIA ATTIE CALIL; BUTLER, MICHAEL; DE FATIMA PIRES AUGUSTO, ELISABETH. Purification of rabies virus glycoprotein produced inDrosophila melanogasterS2cells: An efficient immunoaffinity method. BIOTECHNOLOGY PROGRESS, v. 36, n. 6, AUG 2020. (18/10538-1, 12/24647-0)

OLIVEIRA GUARDALINI, LUIS GIOVANI; DA SILVA CAVALCANTE, PAULO EDUARDO; LEME, JACI; DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; TONSO, ALDO; CALIL JORGE, SORAIA ATTIE; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO. Oxygen uptake and transfer rates throughout production of recombinant baculovirus and rabies virus-like particles. Process Biochemistry, v. 124, p. 12-pg., 2022-12-06. (18/10538-1, 16/22780-6)

GUARDALINI, LUIS GIOVANI OLIVEIRA; DIAS, VINICIUS ARAGAO TEJO; LEME, JACI; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; DA SILVEIRA, SUELLEN REGINA; HO, PAULO LEE; TONSO, ALDO; JORGE, SORAIA ATTIE CALIL; NUNEZ, EUTIMIO GUSTAVO FERNANDEZ. Comparison of chemometric models using Raman spectroscopy for offline biochemical monitoring throughout the VLP-making upstream process. Biochemical Engineering Journal, v. 198, p. 12-pg., 2023-06-21. (18/10538-1, 22/02713-3, 22/04711-8, 16/22780-6)

GUARDALINI, LUIS GIOVANI OLIVEIRA; LEME, JACI; CAVALCANTE, PAULO EDUARDO DA SILVA; DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; JARED, SIMONE GONCALVES SILVA; ANTONIAZZI, MARTA MARIA; ASTRAY, RENATO MANCINI; TONSO, ALDO; NUNEZ, EUTIMIO GUSTAVO FERNANDEZ; et al. Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems. MOLECULAR BIOTECHNOLOGY, v. N/A, p. 11-pg., 2023-05-10. (18/10538-1, 16/22780-6)

LEME, JACI; OLIVEIRA GUARDALINI, LUIS GIOVANI; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; TONSO, ALDO; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO; CALIL JORGE, SORAIA ATTIE. Sf9 Cells Metabolism and Viability When Coinfected with Two Monocistronic Baculoviruses to Produce Rabies Virus-like Particles. MOLECULAR BIOTECHNOLOGY, v. N/A, p. 13-pg., 2022-11-18. (16/22780-6, 18/10538-1)

GUARDALINI, LUIS GIOVANI OLIVEIRA; CAVALCANTE, PAULO EDUARDO DA SILVA; LEME, JACI; DE MELLO, RENATA GOIS; BERNARDINO, THAISSA CONSONI; ASTRAY, RENATO MANCINI; BARBOSA, EDUARDO; DA SILVEIRA, SUELLEN REGINA; HO, PAULO LEE; TONSO, ALDO; et al. Biochemical monitoring throughout all stages of rabies virus-like particles production by Raman spectroscopy using global models. Journal of Biotechnology, v. 363, p. 13-pg., 2023-01-06. (16/22780-6, 18/10538-1)