Analgesic and anti-inflammatory efficacy of lidocaine administered at acupuncture points compared to intravenous route in dogs with pyometra

• Grant number: 18/13116-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: January 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Renata Navarro Cassu
• Grantee: Renata Navarro Cassu
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• Associated researchers: Stelio Pacca Loureiro Luna

Abstract

The aim of this study is to evaluate the analgesic and anti-inflammatory efficacy of lidocaine administered at acupuncture points compared to intravenous (IV) route in dogs. Thirty dogs undergoing ovariohysterectomy diagnosed with pyometra will be included in the study. For the surgical procedure the dogs will be sedated with methadone (0.3 mg/kg) administered by intramuscular (IM) route. Anesthesia will be induced with propofol and maintained with isoflurane/O2. Five minutes after induction of anesthesia the dogs will be distributed into three groups: LIV (n=10): IV lidocaine 1% infusion (loading dose of 2 mg/kg, followed by a constant rate infusion of 50µg/kg/min); LAP (n=10): subcutaneous lidocaine 1% (1 mg/kg) will be injected bilaterally at ST36 and SP6 acupoints; SAL (n=10): IV saline infusion (loading dose of 2 mL/kg, followed by a constant rate infusion of 2mL/kg/min). The degree of analgesia and sedation will be measured before (baseline), 1, 2, 4, 8, 12, 18 and 24 hours after extubation. Rescue analgesia will be provided with IM methadone (0.3 mg/kg). Blood serum levels of interleukin (IL-6 and IL-10) tumor necrosis factor-a (TNF-a) and C-reative protein will be measured at baseline, 6, 12 and 24 hours after surgery. Numerical data will be submitted to analysis of variance followed by Tukey's test for parametric measures and Kruskall-Wallis with post-Dunn test for nonparametric measures (p <0.05). (AU)