Development of bioactive materials based on proteins and natural polymers aiming applications of biomedical interest.

• Grant number: 18/20560-4
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2019
• End date: November 30, 2021
• Field of knowledge: Engineering - Chemical Engineering - Chemical Technology
• Principal Investigator: Marisa Masumi Beppu
• Grantee: Marisa Masumi Beppu
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Bradley Olsen

Abstract

Prostate cancer (PCa) is a silent neoplasm and if diagnosed in its early stages, has high chances of cure. Thus, it is necessary to search for rapid detection methods and new prognostic markers for PCa. In this context, the development of techniques that allow the control of cell adhesion on the surface of substrates is a determining factor for the construction of biomaterials applicable in the biomedical field. A recent study, developed at the Laboratory of Engineering and Chemistry of Products (LEQUIP), explored the interaction between CD44 receptors, expressed in specific cell types, and the hyaluronic acid (HA) molecule in coatings able to promote a selective capture of prostatic tumor cells. CD44 is an extracellular glycoprotein involved in multiple cellular processes (including cell adhesion), and its principal ligand is HA. Using the Layer-by-layer (LbL) technique, a very versatile and low-cost functionalization method, these coatings point to promising possibilities for the development of rapid diagnostic platforms. To reach this goal, it is necessary to establish strategies that explore the biological functionality of nanometric coatings. In this context, the use of the tools of synthetic biology represents innovative ideas for the production of new materials. In this project, it is aimed at the synthesis of recombinant proteins, the expertise of Prof. Brad Olsen from MIT, aiming at the production of new materials exploring the LbL technique. It is expected to promote the incorporation of proteins with biofunctional ligands to the coatings and to evaluate the potential of these new materials for cell adhesion and selective release of drugs. The design of these new materials, combining the versatility of the LbL technique with the biological functionality of synthetic proteins, approaches the frontiers of the knowledge of two different fields of research, making possible the development of coatings for applications of biomedical interest. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATAGLIOLI, ROGERIO A.; ROCHA NETO, JOAO BATISTA M.; LEAO, BRUNO S.; GERMINIANI, LUIZ GUILHERME L.; TAKETA, THIAGO B.; BEPPU, MARISA M.. Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films. Langmuir, v. 36, n. 42, p. 12532-12544, OCT 27 2020. (18/20560-4, 16/10193-9)

TAKETA, THIAGO B.; BATAGLIOLI, ROGERIO A.; ROCHA NETO, JOAO BATISTA M.; BEPPU, MARISA M.. Probing axial metal distribution on biopolymer-based layer-by-layer films for antimicrobial use. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 199, MAR 2021. (16/10193-9, 18/20560-4)

GERMINIANI, LUIZ G. L.; BEPPU, MARISA M.; FRANCO, LUIS F. M.. Densities of the Standard Amino Acids in Aqueous Solutions via Molecular Dynamics Simulations. JOURNAL OF CHEMICAL AND ENGINEERING DATA, v. 67, n. 4, p. 12-pg., 2022-04-14. (18/02713-8, 18/20560-4)

MAIA ROCHA NETO, JOAO BATISTA; SOARES, ANDREY COATRINI; BATAGLIOLI, ROGERIO APARECIDO; CARR, OLIVIA; RODRIGUES COSTA, CARLOS ALBERTO; OLIVEIRA JR, OSVALDO N.; BEPPU, MARISA MASUMI; CARVALHO, HERNANDES F.. Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions. CELLS, v. 9, n. 6, JUN 2020. (18/22214-6, 18/20560-4, 18/18953-8, 16/10193-9, 14/50938-8)

ROCHA NETO, J. B. M.; LIMA, G. G.; FIAMINGO, A.; GERMINIANI, L. G. L.; TAKETA, T. B.; BATAGLIOLI, R. A.; DA SILVEIRA, G. A. T.; DA SILVA, J. V. L.; CAMPANA-FILHO, S. P.; OLIVEIRA JR, O. N.; et al. Controlling antimicrobial activity and drug loading capacity of chitosan-based layer-by-layer films. International Journal of Biological Macromolecules, v. 172, p. 154-161, MAR 1 2021. (16/10193-9, 18/22214-6, 18/20560-4)

NETO, J. B. M. ROCHA; COPES, F.; CHEVALLIER, P.; VIEIRA, R. S.; DA SILVA, J. V. L.; MANTOVANI, D.; BEPPU, M. M.. Polysaccharide-based layer-by-layer nanoarchitectonics with sulfated chitosan for tuning anti-thrombogenic properties. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 213, p. 8-pg., 2022-02-07. (18/20560-4)

BATAGLIOLI, ROGERIO A.; LEAO, BRUNO S.; MILLS, CAROLYN E.; ROCHA NETO, JOAO BATISTA M.; TAKETA, THIAGO B.; OLSEN, BRADLEY D.; BEPPU, MARISA M.. Automated, High-Throughput Screening of Hybrid Elastin-like Polysaccharide/Polypeptide Multilayer Film Deposition. Biomacromolecules, v. N/A, p. 12-pg., 2023-06-22. (16/10193-9, 18/20560-4)