Effect of titanium surface with nanotopography on the interaction between osteoblasts and osteoclasts

• Grant number: 18/17356-6
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2019
• End date: February 28, 2021
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Márcio Mateus Beloti
• Grantee: Márcio Mateus Beloti
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Adalberto Luiz Rosa ; Paulo Tambasco de Oliveira

Abstract

The osseointegration of titanium (Ti) implants is based on bone remodeling, which involves a balance between bone tissue formation by osteoblasts and tissue resorption by osteoclasts. Our group has shown that a Ti surface with nanotopography obtained by chemical conditioning with H2SO4/H2O2 solution is capable of inducing osteoblast differentiation under both osteogenic and non-osteogenic conditions. However, the effect of this surface on differentiation and activity of osteoclasts, and their interaction with osteoblasts have not been investigated yet. In this context, we hypothesized that this nanotopography, in addition to promote osteoblastic differentiation, modulates the osteoblast/osteoclast interaction, inhibiting osteoclastic differentiation. Therefore, the aim of this study is to determine the influence of Ti surface with nanotopography, compared with machined Ti surface (control), on the interaction between osteoblastic and osteoclastic cells. We will evaluate (1) the effect of Ti with nanotopography surface, compared with control Ti surface, on osteoblastic differentiation and osteoclastic formation and activity in isolated cultures of MC3T3-E1 and RAW 264.7 cells, respectively, grown on these surfaces, (2) the effect of MC3T3-E1 cells on osteoclastic formation and activity of RAW 264.7 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models, (3) the effect of RAW 264.7 cells on osteoblastic differentiation of MC3T3-E1 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models, (4) the effect of conditioned medium by MC3T3-E1 cells, grown on Ti surface with nanotopography, compared with control Ti surface, on osteoclastic activity and formation of RAW264.7 cells, (5) the effect of conditioned medium by RAW264.7 cells, grown on Ti surface with nanotopography, compared with control Ti surface, on osteoblastic differentiation of MC3T3-E1 cells. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FLORES ABUNA, RODRIGO PAOLO; OLIVEIRA, FABIOLA SINGARETTI; ADOLPHO, LETICIA FAUSTINO; FERNANDES, ROGER RODRIGO; ROSA, ADALBERTO LUIZ; BELOTI, MARCIO MATEUS. Frizzled 6 disruption suppresses osteoblast differentiation induced by nanotopography through the canonical Wnt signaling pathway. Journal of Cellular Physiology, APR 2020. (16/14171-0, 16/14711-4, 18/17356-6)

BIGHETTI-TREVISAN, RAYANA L.; ALMEIDA, LUCIANA O.; CASTRO-RAUCCI, LARISSA M. S.; GORDON, JONATHAN A. R.; TYE, CORALEE E.; STEIN, GARY S.; LIAN, JANE B.; STEIN, JANET L.; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Titanium with nanotopography attenuates the osteoclast-induced disruption of osteoblast differentiation by regulating histone methylation. BIOMATERIALS ADVANCES, v. 134, p. 13-pg., 2022-03-01. (18/17356-6, 19/09349-2, 17/23888-8)

TREVISAN, R. L. B.; FERRAZ, E. P.; GORDON, J. A. R.; TYE, C.; LIAN, J. B.; STEIN, G. S.; STEIN, J. L.; ROSA, A. L.; BELOTI, M. M.. Effects of Osteoclasts on Transcriptional Regulation of Non-Coding RNAs in Osteoblasts Grown on Titanium with Nanotopography. MOLECULAR BIOLOGY OF THE CELL, v. 34, n. 2, p. 2-pg., 2023-02-01. (17/12622-7, 18/17356-6, 19/09349-2, 17/23888-8)

BIGHETTI-TREVISAN, RAYANA LONGO; FERRAZ, EMANUELA PRADO; FERNANDES SILVA, MARCELLE BEATHRIZ; ZATTA, GUILHERME CREPI; DE ALMEIDA, MARCELO BARROS; ROSA, ADALBERTO LUIZ; BELOTI, MARCIO MATEUS. Effect of osteoblasts on osteoclast differentiation and activity induced by titanium with nanotopography. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 229, p. 9-pg., 2023-09-01. (17/23888-8, 18/17356-6)

FLORES ABUNA, RODRIGO PAOLO; OLIVEIRA, FABIOLA SINGARETTI; ADOLPHO, LETICIA FAUSTINO; FERNANDES, ROGER RODRIGO; ROSA, ADALBERTO LUIZ; BELOTI, MARCIO MATEUS. Frizzled 6 disruption suppresses osteoblast differentiation induced by nanotopography through the canonical Wnt signaling pathway. Journal of Cellular Physiology, v. 235, n. 11, p. 11-pg., 2020-04-02. (16/14171-0, 18/17356-6, 16/14711-4)

ABUNA, RODRIGO P. F.; ALMEIDA, LUCIANA O.; SOUZA, ALANN T. P.; FERNANDES, ROGER R.; SVERZUT, THALES F. V.; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Osteoporosis and osteoblasts cocultured with adipocytes inhibit osteoblast differentiation by downregulating histone acetylation. Journal of Cellular Physiology, v. 236, n. 5, p. 3906-3917, MAY 2021. (18/17356-6, 18/13290-0, 16/14171-0, 17/12622-7, 16/14711-4)

FREITAS, GILEADE P.; SOUZA, ALANN T. P.; LOPES, HELENA B.; TREVISAN, RAYANA L. B.; OLIVEIRA, FABIOLA S.; FERNANDES, ROGER R.; FERREIRA, FERNANDA U.; ROSE, FELIPE A.; BELOTI, MARCIO M.; ROSA, ADALBERTO L.. Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation. BIO-PROTOCOL, v. 10, n. 4, FEB 20 2020. (18/17356-6, 17/12622-7)