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Bioavailability, bioaccessibility, and speciation of nutrients and inorganic contaminants in meat and fish

Abstract

With activities facing the developed and validation of analytical methods to evaluate the nutritional quality of meat and fish, this proposal aims to provide analytical information to meet the increasingly restricted quality requirements of the product, providing additional information regarding the behavior of the species studied during food processing. The development of different procedures for preparation and analysis of samples will involve particular characteristics to each desired result, such speciation and fractionation chemical analysis in addition to the total contents, bioavailable and bioaccessible fractions of nutrients and inorganic contaminants. The determinations will be performed by mass spectrometry, optical emission spectrometry with argon plasma and microwave induced plasma (ICP-MS, ICP OES, and MIP OES), and ion chromatography (IC). The chromatographic coupling of ICP-MS in the chemical speciation of As, Hg and Cr in fish and shrimp, the determination of organic and inorganic forms of Mo, P and S in sheep meat and the bioaccessible and bioavailable fractions of nutrients and possible contaminants in samples of sheep, tilapia and shrimp will be evaluated. The dialysis and CaCo-2 cellular models will be used for the determination of bioavailable and bioavailable fractions, respectively.The static and dynamic digestion models were compared. The training of human resources, able to develop research activities related to nutritional chemistry is also included in this proposal. The activities will be carried out at Embrapa Pecuária Sudeste by students who work under the guidance of the proponent in the Group of Applied Instrumental Analysis, GAIA (http://www.gaia.ufscar.br). There will be a constant discussion of the stages and activities with researchers who work on the project "Production Chains of Aquaculture in Brazil," focused on the development of structural actions of the fishing chain and with those responsible for the project of crossbreeding of sheep, developed at Embrapa. The results will be presented at congresses and published in the form of regulations, research bulletins, and scientific articles. The proposal presents a transversal characteristic and meets the needs of the legislation, focused on traceability and food quality. The target is to provide subsidies for the establishment of methods for the correct use of laboratory procedures. Improving the quality of the analytical results, it will be possible increase productivity and raise the quality of the products of the sheep and fish meat chains, such as the increase of efficiency in the use of the inputs, the improvement of nutritional quality and the reduction of anti-nutritional or toxic factors. (AU)

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VEICULO: TITULO (DATA)

Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOREIRA, LUANA S.; COSTA, FLORIATAN S.; MACHADO, RAQUEL C.; NOGUEIRA, ANA RITA A.; GONZALEZ, MARIO H.; DA SILVA, ERIK G. P.; AMARAL, CLARICE D. B. Self-organizing map applied to the choice of internal standards for the determination of Cd, Pb, Sn, and platinum group elements by inductively coupled plasma mass spectrometry. Talanta, v. 233, OCT 1 2021. Web of Science Citations: 0.
MACHADO, RAQUEL C.; SILVA, ANA BEATRIZ S.; ANDRADE, DANIEL F.; PINHEIRO, FERNANDA C.; WILLIAMS, CHARLES B.; DONATI, GEORGE L.; NOGUEIRA, ANA RITA A.; PEREIRA-FILHO, EDENIR R. Alternative Approaches Applied to Inductively Coupled Plasma Techniques: Multi-Flow and Two-Flow Calibration. Journal of the Brazilian Chemical Society, v. 32, n. 4, p. 842-851, APR 2021. Web of Science Citations: 0.
SILVA, ANA BEATRIZ S.; SA, IVERO P.; HIGUERA, JULYMAR M.; NOGUEIRA, ANA RITA A. Feasibility of Dispersive Liquid-Liquid Microextraction for Molybdenum Determination in Lamb Meat by MIP OES. FOOD ANALYTICAL METHODS, v. 14, n. 3, p. 496-502, MAR 2021. Web of Science Citations: 0.
DE SA, IVERO PITA; DE SOUZA, GILBERTO BATISTA; NOGUEIRA, ANA RITA DE ARAUJO. Chromium speciation in organic fertilizer by cloud point extraction and optimization through experimental Doehlert design as support for legislative aspects. Microchemical Journal, v. 160, n. A JAN 2021. Web of Science Citations: 0.
HIGUERA, JULYMAR M.; SILVA, ANA BEATRIZ S.; HENRIQUE, WIGNEZ; ESTEVES, SERGIO N.; BARIONI, JR., WALDOMIRO; DONATI, GEORGE L.; NOGUEIRA, ANA RITA A. Effect of Genetic Crossing and Nutritional Management on the Mineral Composition of Carcass, Blood, Leather, and Viscera of Sheep. BIOLOGICAL TRACE ELEMENT RESEARCH, JAN 2021. Web of Science Citations: 0.
MOREIRA, LUANA S.; SA, IVERO P.; MACHADO, RAQUEL C.; NOGUEIRA, ANA RITA A.; DA SILVA, ERIK G. P.; AMARAL, CLARICE D. B. Coprecipitation magnesium(II) hydroxide as a strategy of pre-concentration for trace elemental determination by microwave-induced plasma optical emission spectrometry. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, v. 169, JUL 2020. Web of Science Citations: 0.
CARLA M. BOSSU; EVELINE A. MENEZES; ANA RITA A. NOGUEIRA. BIOACESSIBILIDADE DE ZINCO, CÁLCIO E FÓSFORO EM EXTRATO DE SOJA E AMOSTRAS DE LEITE BOVINO, CAPRINO E OVINO. Química Nova, v. 43, n. 6, p. 718-722, Jun. 2020.
SILVA, ANA BEATRIZ S.; HIGUERA, JULYMAR M.; BRAZ, CARLOS EDUARDO M.; MACHADO, RAQUEL C.; NOGUEIRA, ANA RITA A. Evaluation of different nebulizers performance on microwave-induced plasma optical emission spectrometry. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, v. 168, JUN 2020. Web of Science Citations: 0.
DE HIGUERA, JULYMAR MARCANO; SANTOS DA SILVA, ANA BEATRIZ; DE OLIVEIRA, ALINE FERNANDES; DE ARAUJO NOGUEIRA, ANA RITA. Multi-elemental determination in meat samples using multi-isotope calibration strategy by ICP-MS. Food Chemistry, v. 303, JAN 15 2020. Web of Science Citations: 6.
HIGUERA, JULYMAR M.; SILVA, ANA BEATRIZ S.; NOGUEIRA, ANA RITA A. Multi Energy Calibration: A Practical Method for Determination of Macro and Micro Nutrients in Meat by ICP OES. Journal of the Brazilian Chemical Society, v. 30, n. 12, SI, p. 2575-2581, DEC 2019. Web of Science Citations: 0.
JULYMAR M. HIGUERA; ANA BEATRIZ S. SILVA; ANA RITA A. NOGUEIRA. Multi-Energy Calibration: A Practical Method for Determination of Macro and Micro Nutrients in Meat by ICP OES. Journal of the Brazilian Chemical Society, v. 30, n. 12, p. 2575-2581, Dez. 2019.

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