Establishment of a synthetic bacterial community and its genetic association with tropical maize

• Grant number: 19/04697-2
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2019
• End date: August 31, 2021
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: João Lúcio de Azevedo
• Grantee: João Lúcio de Azevedo
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Maria Carolina Quecine Verdi ; Roberto Fritsche Neto

Abstract

In order to obtain a sustainable agriculture, totally or partially reducing the use of mineral fertilizers, several studies have been conducted addressing the association of plants-microorganisms with the purpose of promoting plant development. In this context, plant growth promoting bacteria (PGPB) have been isolated from the rhizospheric or endophytic region of plants and used as inoculants. However, the recent panorama of the inoculants use in Brazil has shown some inconsistency comparing in vitro and in vivo assays, sometime resulting in negative effects at the field level. The advancement of molecular sequencing technologies of DNA and RNA has demonstrated that inoculant efficiency is closely associated with microbe-microbe interactions, in which the microbiome itself buffers the effect of invasion and stability of inoculating agents in the environment. Thus, the aim of this project is to create a synthetic bacterial community, using key plant growth promotion bacterial strains, as the mainframe to modulate the taxonomy and functionality of the indigenous ecosystem microbiota, therefore creating mechanisms to favor the survival of the microbial inoculants and its association with plant, in order to improve the plant sanity. For this, a joint approach will be carried out on ecological theories, reductionist methodologies of microbial community, metagenomics and metatranscriptomics sequencing technologies. Moreover, the genetic control of SNPs (single nucleotide polymorphisms) to maize - synthetic bacterial community - PGPB will be investigated. A total of 384 tropical maize linages will be evaluated under greenhouse condition with and without inoculation. The phenotyping will be sampled by traditional and images (high resolution) methodologies. To identification of SNPs in linkage disequilibrium and characters related with plant growth promotion, molecular and phenotypic data will be evaluated by Genome-wide association studies (GWAS) (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRAREZI, JESSICA APARECIDA; CARVALHO-ESTRADA, PAULA DE ALMEIDA; BATISTA, BRUNA DURANTE; ANICETO, RAFAEL MARTINS; PROHMANN TSCHOEKE, BRUNO AUGUSTO; DE MAIA ANDRADE, PEDRO AVELINO; LOPES, BRUNA DE MOURA; BONATELLI, MARIA LETICIA; ODISI, ESTACIO JUSSIE; AZEVEDO, JOAO LUCIO; et al. Effects of inoculation with plant growth-promoting rhizobacteria from the Brazilian Amazon on the bacterial community associated with maize in field. APPLIED SOIL ECOLOGY, v. 170, FEB 2022. (15/01188-9, 19/04697-2, 19/25720-2)

YASSUE, RAFAEL MASSAHIRO; CARVALHO, HUMBERTO FANELLI; GEVARTOSKY, RAYSA; SABADIN, FELIPE; SOUZA, PEDRO HENRIQUE; BONATELLI, MARIA LETICIA; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA; FRITSCHE-NETO, ROBERTO. On the genetic architecture in a public tropical maize panel of the symbiosis between corn and plant growth-promoting bacteria aiming to improve plant resilience. MOLECULAR BREEDING, v. 41, n. 10, OCT 2021. (17/24327-0, 19/04697-2)

FERRAREZI, JESSICA APARECIDA; DEFANT, HELOISA; DE SOUZA, LEANDRO FONSECA; AZEVEDO, JOAO LUCIO; HUNGRIA, MARIANGELA; QUECINE, MARIA CAROLINA. Meta-omics integration approach reveals the effect of soil native microbiome diversity in the performance of inoculant Azospirillum brasilense. FRONTIERS IN PLANT SCIENCE, v. 14, p. 15-pg., 2023-06-29. (19/25720-2, 19/04697-2, 21/11983-1)

YASSUE, RAFAEL MASSAHIRO; GALLI, GIOVANNI; FRITSCHE-NETO, ROBERTO; MOROTA, GOTA. Classification of plant growth-promoting bacteria inoculation status and prediction of growth-related traits in tropical maize using hyperspectral image and genomic data. CROP SCIENCE, v. 63, n. 1, p. 13-pg., 2022-11-29. (17/19407-4, 17/24327-0, 19/04697-2)