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Understanding the c-di-GMP signaling networks and the Type II secretion system present into the human pathogen Leptospira interrogans Copenhageni

Grant number: 19/00195-2
Support type:Regular Research Grants
Duration: August 01, 2019 - July 31, 2021
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Cristiane Rodrigues Guzzo Carvalho
Grantee:Cristiane Rodrigues Guzzo Carvalho
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):19/18356-2 - Expression and purification of the GspC-GspD complex of the type II secretion system of Leptospira interrogans Copenhgeni, BP.TT


The goals of the project are the study of proteins of the human pathogen Leptospira interrogans. This bacterium is the causative agent of leptospirosis, a re-emerging infectious disease considered a zoonosis worldwide that affects several animals including humans. More than 500,000 cases of severe leptospirosis are reported per year, and in severe cases the patient ends up dying. Due to the medical importance of this pathogen functional and structural studies of the c-di-GMP signalling pathways and the type II secretion system will contribute to the understanding of the molecular bases involved in the biology of this spirochete and may contribute to the elucidation of its virulence pathways. Possible results from our research may result in the creation of drugs to combat leptospirosis infection. However, this is not the goal of this project. The project proposed here has two main objectives: 1- Structural and functional study of proteins involved in the synthesis, degradation, binding to the second bacterial messenger, c-di-GMP and 2- structural and functional study of the Leptospira type II secretion system, the only secretory system noted in its genome.Our studies will be done using different techniques of structural biology such as X-ray diffraction and cryo-electron microscopy, as well as several techniques of biochemistry of macromolecules, such as Western Blotting, kinetic enzymatic, among others. In general, this proposal encompasses well established techniques in our research group and innovative techniques such as cryo-electron microscopy. The results obtained in this project will contribute to the scientific community with the projection of publications in journals of high scientific impact. (AU)

Articles published in other media outlets (1 total):
Jornal da USP online: Pesquisadores do Instituto de Ciências Biomédicas descobrem nova família de toxina bacteriana (03/Jul/2020)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SIBINELLI-SOUSA, STEPHANIE; HESPANHOL, JULIA T.; NICASTRO, GIANLUCCA G.; MATSUYAMA, BRUNO Y.; MESNAGE, STEPHANE; PATEL, ANKUR; DE SOUZA, ROBSON F.; GUZZO, CRISTIANE R.; BAYER-SANTOS, ETHEL. A Family of T6SS Antibacterial Effectors Related to L,D-Transpeptidases Targets the Peptidoglycan. CELL REPORTS, v. 31, n. 12 JUN 23 2020. Web of Science Citations: 0.
ALINE DIAS DA, PURIFICACAO; NATHALIA MARINS DE, AZEVEDO; GABRIEL GUARANY DE, ARAUJO; ROBSON FRANCISCO DE, SOUZA; RODRIGUES, GUZZO CRISTIANE. The World of Cyclic Dinucleotides in Bacterial Behavior. Molecules, v. 25, n. 10 MAY 2020. Web of Science Citations: 0.

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