Dentin remineralization with the use of composite containing calcium orthophosphate particles associated with a Polymer-Induced Liquid Precursor (PILP): material development, in vitro and randomized clinical studies

• Grant number: 19/04737-4
• Support Opportunities: Research Projects - Thematic Grants
• Start date: October 01, 2020
• End date: September 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Roberto Ruggiero Braga
• Grantee: Roberto Ruggiero Braga
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Daniela Rios Honório ; Marcelo Giannini
• Associated researchers: Bruna Marin Fronza ; Marília Afonso Rabelo Buzalaf ; Stefan Habelitz
• Associated research grant(s): 24/01640-8 - In vitro analysis of dentin remineralization using an experimental composite containing calcium silicate particles, AP.R
• Associated scholarship(s): 24/03730-4 - Physicochemical Characterization of Resin Composites Containing Calcium Orthophosphate or Bioactive Glass-ceramic and Their Effects on Dentin Remineralization in vitro, BP.MS; 24/10714-5 - Effects of the ratio between BisGMA and TEGDMA on the ability to incorporate calcium orthophosphate particles into restorative composites, BP.IC; 23/12176-8 - Contribution of the particle-matrix bonding to the mechanical characteristics of composites containing calcium orthophosphate, BP.IC; + associated scholarships 23/18387-0 - Resistance to toothbrush wear and surface roughness of resin composites containing DCPD - in vitro study, BP.IC; 22/15593-6 - Dentin remineralization using a composite containing calcium orthophosphate particles associated to a mineral precursor in liquid phase (polymer-induced liquid-precursor, PILP): randomized clinical trial, BP.PD; 21/09297-2 - Effect of the ratio between DCPD and barium glass particles on the fracture toughness (KIc) of experimental composites, BP.IC; 21/09657-9 - Dentin functional remineralization using an biomimetic analog, associated or not with a composite containing calcium orthophosphate, BP.DD; 20/12761-0 - In vitro analysis of dentin remineralization using glass ionomer cement with or without calcium orthophosphate particles or with mineral trioxide aggregate, BP.DD; 20/10208-1 - Effect of polyaspartic acid on dentin regeneration in rat molars, BP.MS; 20/13983-6 - Effect of temperature, pH and precursors concentrations on the synthesis of DCPD particles and its application on a remineralizing resin composite, BP.PD - associated scholarships

Abstract

The objective of this project is to develop a resin-based composite containing DCPD particles (dicalcium phosphate dihydrate, CaHPO4.2H2O) to be used in minimally invasive restorations, and evaluate its efficacy in the functional (or biomimetic) remineralization of artificially demineralized human dentin, combined or not with the PILP process. DCPD particles will be synthesized by co-precipitation, characterized and used in resin composite formulations associating DCPD particles and reinforcing particles at different ratios. Composites will be tested for degree of conversion, fracture toughness and ion release up to 14 days. The material with the most favorable relation between KIc and ion release will be used in the remineralization studies. For the in vitro phase, dentin blocks (previously immersed for 66 hours in acetate buffer, pH 5.0) will receive a layer of composite, combined or not to a pre-treatment with the PILP solution. After 14 days of immersion in simulated body fluid, dentin remineralization at the interface with the restorative material will be evaluated by attenuated total reflectance mid-infrared spectroscopy (ATR-FTIR). The recovery of mechanical properties will be evaluated by nanoindentation. Additionally, cross-section of dentin subject to the different treatments will be observed under transmission electron microscopy. For the randomized clinical phase, children with mixed dentition presenting carious lesions in primary molars (ICDAS 5 or 6) will be ascribed to one of the four treatments described in the in vitro phase after selective caries removal. After exfoliation, teeth will be analyzed according to the same methods used for the in vitro specimens. Statistical treatment of the data will be defined after preliminary testing for normality and homogeneity of variance. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMPOS, AMANDA LOPES; VELA, BEATRIZ FONSECA; BORGES, LINCOLN PIRES SILVA; TRINCA, RAFAEL BERGAMO; PFEIFER, CARMEM SILVIA; BRAGA, ROBERTO RUGGIERO. Compositional boundaries for functional dental composites containing calcium orthophosphate particles. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 144, p. 9-pg., 2023-06-09. (21/09297-2, 20/13983-6, 19/04737-4, 21/09657-9)

TRINCA, RAFAEL BERGAMO; OLIVEIRA, BEATRIZ ALMEIDA; VILELA, HANDIALLY DOS SANTOS; BRAGA, ROBERTO RUGGIERO. Effect of calcium orthophosphate particle size and CaP:glass ratio on optical, mechanical and physicochemical characteristics of experimental composites. Dental Materials, v. 39, n. 9, p. 9-pg., 2023-08-23. (19/04737-4, 20/12761-0, 20/13983-6)

RESENDE, MARIANA C. A.; VILELA, HANDIALLY S.; CHIARI, MARINA D. S.; TRINCA, RAFAEL B.; SILVA, FLAVIA R. O.; BRAGA, ROBERTO R.. Physicochemical characterization of experimental resin-based materials containing calcium orthophosphates or calcium silicate. Dental Materials, v. 40, n. 10, p. 8-pg., 2024-09-13. (20/05986-5, 19/04737-4, 20/12761-0, 22/08411-9)

TRINCA, RAFAEL BERGAMO; VELA, BEATRIZ FONSECA; VILELA, HANDIALLY DOS SANTOS; BRAGA, ROBERTO RUGGIERO. Ion release mechanisms in composites containing CaP particles and hydrophilic monomers. Dental Materials, v. 40, n. 7, p. 9-pg., 2024-06-14. (19/04737-4, 21/09297-2, 20/13983-6)

DA SILVA, R. A. A.; TRINCA, R. B.; VILELA, H. S.; BRAGA, R. R.. Composite Containing Calcium Phosphate Particles Functionalized with 10-MDP. JOURNAL OF DENTAL RESEARCH, v. 103, n. 4, p. 7-pg., 2024-01-29. (22/03854-0, 19/04737-4, 20/12761-0, 20/13983-6)

CAMPOS, AMANDA LOPES; CHIARI, MARINA DAMASCENO SOUZA DE CARVALHO; VELA, BEATRIZ FONSECA; TRINCA, RAFAEL BERGAMO; BALBINOT, GABRIELA DE SOUZA; COLLARES, FABRICIO MEZZOMO; BRAGA, ROBERTO RUGGIERO. Dentin remineralization induced by experimental composites containing calcium orthophosphate particles. Dental Materials, v. 41, n. 3, p. 7-pg., 2025-03-01. (20/13983-6, 19/04737-4, 21/09657-9)

VILELA, HANDIALLY SANTOS; RODRIGUES, MARCELA CHARANTOLA; FRONZA, BRUNA MARIN; TRINCA, RAFAEL BERGAMO; VICHI, FLAVIO MARON; BRAGA, ROBERTO RUGGIERO. Effect of Temperature and pH on Calcium Phosphate Precipitation. Crystal Research and Technology, v. 56, n. 12, OCT 2021. (20/13983-6, 18/21023-2, 19/04737-4, 19/01701-9)