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Dentin remineralization with the use of composite containing calcium orthophosphate particles associated with a Polymer-Induced Liquid Precursor (PILP): material development, in vitro and randomized clinical studies

Grant number: 19/04737-4
Support Opportunities:Research Projects - Thematic Grants
Duration: October 01, 2020 - September 30, 2025
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Roberto Ruggiero Braga
Grantee:Roberto Ruggiero Braga
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Pesquisadores principais:
Daniela Rios Honório ; Marcelo Giannini
Associated researchers:Bruna Marin Fronza ; Marília Afonso Rabelo Buzalaf ; Stefan Habelitz
Associated scholarship(s):23/12176-8 - Contribution of the particle-matrix bonding to the mechanical characteristics of composites containing calcium orthophosphate, BP.IC
23/18387-0 - Resistance to toothbrush wear and surface roughness of resin composites containing DCPD - in vitro study, BP.IC
22/15593-6 - Dentin remineralization using a composite containing calcium orthophosphate particles associated to a mineral precursor in liquid phase (polymer-induced liquid-precursor, PILP): randomized clinical trial, BP.PD
+ associated scholarships 21/09297-2 - Effect of the ratio between DCPD and barium glass particles on the fracture toughness (KIc) of experimental composites, BP.IC
21/09657-9 - Dentin functional remineralization using an biomimetic analog, associated or not with a composite containing calcium orthophosphate, BP.DD
20/12761-0 - In vitro analysis of dentin remineralization using glass ionomer cement with or without calcium orthophosphate particles or with mineral trioxide aggregate, BP.DD
20/10208-1 - Effect of polyaspartic acid on dentin regeneration in rat molars, BP.MS
20/13983-6 - Effect of temperature, pH and precursors concentrations on the synthesis of DCPD particles and its application on a remineralizing resin composite, BP.PD - associated scholarships


The objective of this project is to develop a resin-based composite containing DCPD particles (dicalcium phosphate dihydrate, CaHPO4.2H2O) to be used in minimally invasive restorations, and evaluate its efficacy in the functional (or biomimetic) remineralization of artificially demineralized human dentin, combined or not with the PILP process. DCPD particles will be synthesized by co-precipitation, characterized and used in resin composite formulations associating DCPD particles and reinforcing particles at different ratios. Composites will be tested for degree of conversion, fracture toughness and ion release up to 14 days. The material with the most favorable relation between KIc and ion release will be used in the remineralization studies. For the in vitro phase, dentin blocks (previously immersed for 66 hours in acetate buffer, pH 5.0) will receive a layer of composite, combined or not to a pre-treatment with the PILP solution. After 14 days of immersion in simulated body fluid, dentin remineralization at the interface with the restorative material will be evaluated by attenuated total reflectance mid-infrared spectroscopy (ATR-FTIR). The recovery of mechanical properties will be evaluated by nanoindentation. Additionally, cross-section of dentin subject to the different treatments will be observed under transmission electron microscopy. For the randomized clinical phase, children with mixed dentition presenting carious lesions in primary molars (ICDAS 5 or 6) will be ascribed to one of the four treatments described in the in vitro phase after selective caries removal. After exfoliation, teeth will be analyzed according to the same methods used for the in vitro specimens. Statistical treatment of the data will be defined after preliminary testing for normality and homogeneity of variance. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VILELA, HANDIALLY SANTOS; RODRIGUES, MARCELA CHARANTOLA; FRONZA, BRUNA MARIN; TRINCA, RAFAEL BERGAMO; VICHI, FLAVIO MARON; BRAGA, ROBERTO RUGGIERO. Effect of Temperature and pH on Calcium Phosphate Precipitation. Crystal Research and Technology, v. 56, n. 12, . (20/13983-6, 18/21023-2, 19/04737-4, 19/01701-9)
CAMPOS, AMANDA LOPES; VELA, BEATRIZ FONSECA; BORGES, LINCOLN PIRES SILVA; TRINCA, RAFAEL BERGAMO; PFEIFER, CARMEM SILVIA; BRAGA, ROBERTO RUGGIERO. Compositional boundaries for functional dental composites containing calcium orthophosphate particles. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 144, p. 9-pg., . (21/09297-2, 20/13983-6, 19/04737-4, 21/09657-9)

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