Machine perfusion and disinfection for kidney transplant

• Grant number: 19/23070-0
• Support type: Research Grants - Innovative Research in Small Business - PIPE
• Duration: August 01, 2020 - April 30, 2021
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Carolina de Paula Campos
• Grantee: Carolina de Paula Campos
• Company: Carolina de Paula Campos
• CNAE: Atividades de atenção à saúde humana não especificadas anteriormente
• City: São Carlos
• Assoc. researchers: Cristina Kurachi ; José Dirceu Vollet Filho ; Natalia Mayumi Inada ; Vanderlei Salvador Bagnato
• Associated scholarship(s): 20/10799-0 - Portable perfusion and decontamination machine for kidney transplantation, BP.PIPE

Abstract

Currently, in Brazil, the majority of kidney transplants are performed in static cold storage organ (CS). However, studies have shown that the use of a machine perfusion (MP), which promotes the circulation of preservation solution through the organ, improves kidney transplantation (KT) outcome, reducing costs and post-transplant complications. The MP used in Brazil are imported since there is no national company that produces this equipment. In addition, infections transmited from donor to recipient aggravate the health condition of the immunosuppressed patient, causing discomfort and expenses with medication and hospitalization.The aim for PIPE phase 1 is to couple a circulating fluid decontamination device to a commercial portable perfusion machine and test it for the decontamination of a kidney preservation solution. The device acts by germicidal action through ultraviolet C (UVC) light sources. The device and the machine where it will be coupled will be addapted, and a characterization of the irradiation parameters will be performed. Microbiological tests will be performed to verify the effect of decontamination of the kidney preservation solution. The microbiological reduction protocols will be selected in order to ensure the quality of the preservation solution.Therefore, quality tests and cytotoxicity tests on mammalian cells will be performed with the preservation solution after irradiation. In the final stage of Phase 1, we hope to validate the decontamination principle, obtaining reduction of the microbiological load and maintaining the quality of the kidney preservation solution. Thus, in the next phase we will work on the development of the machine perfusion and disinfection (MPD) for kidney transplantation prototype. The proven benefits of the kidney transplant using MP, along with the growing public policy of organ donation incentive and the evidences of transplante better cost-effectiveness over other renal replacement therapies indicate a great business potential for the product used in this project. (AU)