Maximization of the anti-caries and antibiofilm effect of conventional substances: evaluation in a polymicrobial biofilm model

• Grant number: 19/08375-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2020
• End date: February 28, 2023
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Fernanda Lourenção Brighenti
• Grantee: Fernanda Lourenção Brighenti
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Alberto Carlos Botazzo Delbem ; Marcelle Danelon

Abstract

Dental caries is a chronic, multifactorial disease resulting from the presence of biofilms and fermentable carbohydrates. Suppressing the hegemony of cariogenic species in the dental biofilm can be an effective alternative for its control, which can be achieved using different strategies. The present study is divided into three phases. The aim of the first phase will be to define the most appropriate concentration for the associated use of three substances of recognized anticariogenic action. In the second phase, the antibiofilm and anti-caries properties the three substances alone or in combination will be evaluated using enamel or dentin blocks. In the third phase, the mechanisms of action involved in the antibiofilm and anti-caries activity of the substances alone or in combination will be evaluated. Polymicrobial biofilms obtained from a donor's saliva will be used. Biofilms will grow in an active adhesion model, using intermittent sucrose exposure. In phase 1, the biofilms will grow on glass discs. The microbial composition and acidogenicity of the biofilms from 16 experimental groups will be assessed. In phase 2, acidogenicity and microbial composition of biofilms will be evaluated, as well as demineralization of the enamel surface and the depth of the lesion formed. In phase 3, the chemical composition of the enamel and dentin surface, the concentration of insoluble extracellular polysaccharides in the biofilm matrix, the biofilm biomass and the concentration of calcium, fluoride and phosphate in the biofilm fluid will be evaluated. The data will be analyzed using the GraphPad Prism version 3.02. A test of normality of the sample distribution and homogeneity of variances (p <0.05) will be performed. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; DE ANNUNZIO, SARAH RAQUEL; FRANCA, MARIA GLEIZIANE ARAUJO; SILVA, MARIA GORETTI DE VASCONCELOS; CAVALHEIRO, ALBERTO JOSE; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest. PHARMACEUTICS, v. 15, n. 1, p. 16-pg., 2023-01-01. (13/07600-3, 22/12828-2, 14/50926-0, 20/07110-0, 19/08375-0, 18/09088-1, 18/18440-0, 18/23015-7, 21/01191-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FRANCA, GABRIELA GOMES; DE ANNUNZIO, SARAH RAQUEL; KOPP, WILLIAN; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms. PATHOGENS, v. 12, n. 2, p. 19-pg., 2023-02-01. (19/08375-0, 18/23015-7, 18/18440-0, 13/07276-1, 21/01191-0, 20/07110-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BASSO, FERNANDA GONCALVES; FONTANA, CARLA RAQUEL; APARECIDA GIRO, ELISA MARIA; BRIGHENTI, FERNANDA LOURENCAO. A systematic review and meta-analysis of the effect of photodynamic therapy for the treatment of oral mucositis. Photodiagnosis and Photodynamic Therapy, v. 34, JUN 2021. (18/18440-0, 20/07110-0, 21/01191-0, 19/08375-0, 18/23015-7)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; BASSO, FERNANDA GONCALVES; BRIGHETI, FERNANDA LOURENCAO. Photodynamic therapy for treating infected skin wounds: A systematic review and meta-analysis from randomized clinical trials. Photodiagnosis and Photodynamic Therapy, v. 40, p. 11-pg., 2022-12-01. (13/07276-1, 18/23015-7, 21/01191-0, 20/07110-0, 19/08375-0, 18/18440-0)

THALITA MENDES; LUCIANA SOLERA SALES; MARCELLE DANELON; FERNANDA LOURENÇÃO BRIGHENTI. Seleção de doadores de saliva para crescimento de biofilme in vitro. Rev. odontol. UNESP, v. 52, 2023-12-04. (21/12931-5, 19/26066-4, 19/08375-0)

DE FARIAS, ALINE LEITE; ARBELAEZ, MARIA ISABEL AMAYA; MENEGUIN, ANDREIA BAGLIOTTI; BARUD, HERNANE DA SILVA; BRIGHENTI, FERNANDA LOURENCAO. ucoadhesive controlled-release formulations containing morin for the control of oral biofilm. BIOFOULING, v. 38, n. 1, DEC 2021. (19/08375-0)

SALES, LUCIANA SOLERA; MIRANDA, MARINA LINS; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; MILWARD, MICHAEL; BRIGHENTI, FERNANDA LOURENCAO. Effect of the technique of photodynamic therapy against the main microorganisms responsible for periodontitis: A systematic review of in-vitro studies. ARCHIVES OF ORAL BIOLOGY, v. 138, p. 13-pg., 2022-03-28. (19/26066-4, 21/12931-5, 20/07110-0, 19/08375-0, 21/01191-0)

GABBAI-ARMELIN, PAULO R.; SALES, LUCIANA S.; FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; DE OLIVEIRA, JULIANA R.; GIRO, ELISA M. A.; BRIGHENTI, FERNANDA L.. A systematic review and meta-analysis of the effect of thymol as an anti-inflammatory and wound healing agent A review of thymol effect on inflammation and wound healing. Phytotherapy Research, v. 36, n. 9, p. 29-pg., 2022-07-18. (20/07110-0, 19/08375-0, 21/01191-0, 19/26066-4)

GABBAI-ARMELIN, PAULO ROBERTO; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; SALES, LUCIANA SOLERA; BARBOSA, ELIS RODRIGUES OLIVEIRA; MIRANDA, MARINA LINS; SALOMAO, KARINA BORGES; BRIGHENTI, FERNANDA LOURENCAO. COVID-19 (SARS-CoV-2) infection and thrombotic conditions: A systematic review and meta-analysis. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, v. 51, n. 6, APR 2021. (21/01191-0, 19/08375-0, 19/26066-4, 18/18440-0, 20/07110-0)

SALES, LUCIANA SOLERA; GIMENES, MILENA DA SILVA; MENEGUIN, ANDREIA BAGLIOTTI; BARUD, HERNANE DA SILVA; ACHCAR, JORGE ALBERTO; BRIGHENTI, FERNANDA LOURENCAO. Development of multiparticulate systems based on natural polymers for morin controlled release. International Journal of Biological Macromolecules, v. 228, p. 12-pg., 2022-12-23. (19/26066-4, 19/19817-3, 17/50334-3, 21/12931-5, 19/08375-0, 13/07276-1)

Filed patent(s) as a result of this research project