Characterization molecular and phenotypic of opportunistic bacteria: development of vaccines and drugs using subtractive proteomics

• Grant number: 20/11964-4
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2021
• End date: April 30, 2023
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Maria Cristina da Silva Pranchevicius
• Grantee: Maria Cristina da Silva Pranchevicius
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Anderson Ferreira da Cunha ; André Pitondo da Silva ; Louise Teixeira Cerdeira

Abstract

The evolution, the increased prevalence and the spread of bacteria resistant to multiple antibiotics, aggravated by the lack of new drugs, produces a serious global problem for the control and management of infections. Molecular techniques have been used to mitigate some of these problems. Most of these techniques can be used to identify genes and genetic elements responsible for the dissemination of antibiotic resistance determinants and virulence factors, and also allow the identification of potential therapeutic candidates that can be used in the clinical management of infected patients. Therefore, we are proposing molecular studies on the antimicrobial resistance profile; virulence; phylogenetic analyzes; sequencing, and genome analysis of opportunistic bacteria such as Serratia marcescens, Klebsiella pneumoniae and Staphylococci strains. We also intend, through subtractive proteomics and reverse vaccinology techniques, to find potential drug targets and vaccine candidates that can be used against S. marcescens, an opportunistic pathogen, that have become multidrug resistant (MDR) and have been associated with serious infections and high mortality rate. The data obtained will provide important information about the reservoirs of resistance and virulence genes, the genetic relationships, and will be useful for identification of new therapeutic compounds against MDR bacteria. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES, GABRIELA GUERRERA; CAMPANINI, EMELINE BONI; FERREIRA, ROUMAYNE LOPES; DAMAS, MARCELO SILVA FOLHAS; RODRIGUES, SAULO HENRIQUE; CAMPOS, LESLIE CAMELO; GALVAO, JUCIMARIA DANTAS; FUENTES, ANDREA SOARES DA COSTA; FREIRE, CAIO CESAR DE MELO; MALAVAZI, IRAN; et al. Brevundimonas brasiliensis sp. nov.: a New Multidrug-Resistant Species Isolated from a Patient in Brazil. MICROBIOLOGY SPECTRUM, v. 11, n. 3, p. 21-pg., 2023-04-17. (18/20697-0, 20/11964-4, 21/08423-4, 18/24213-7)

FOLHAS DAMAS, MARCELO SILVA; MAZUR, FERNANDO GABRIEL; DE MELO FREIRE, CAIO CESAR; DA CUNHA, ANDERSON FERREIRA; PRANCHEVICIUS, MARIA-CRISTINA DA SILVA. A Systematic Immuno-Informatic Approach to Design a Multiepitope-Based Vaccine Against Emerging Multiple Drug Resistant Serratia marcescens. FRONTIERS IN IMMUNOLOGY, v. 13, p. 24-pg., 2022-03-14. (18/24213-7, 20/11964-4, 18/20697-0)

RODRIGUES, SAULO HENRIQUE; NUNES, GUSTAVO DANTAS; SOARES, GABRIELA GUERRERA; FERREIRA, ROUMAYNE LOPES; DAMAS, MARCELO SILVA FOLHAS; LAPREGA, PEDRO MENDES; SHILLING, REBECCA ELIZABETH; CAMPOS, LESLIE CAMELO; DA COSTA, ANDREA SOARES; MALAVAZI, IRAN; et al. First report of coexistence of blaKPC-2 and blaNDM-1 in carbapenem-resistant clinical isolates of Klebsiella aerogenes in Brazil. FRONTIERS IN MICROBIOLOGY, v. 15, p. 21-pg., 2024-02-14. (21/00425-8, 22/16872-6, 18/20697-0, 21/08423-4, 22/01223-2, 18/24213-7, 23/08917-2, 20/11964-4, 22/12429-0)

DAMAS, MARCELO SILVA FOLHAS; FERREIRA, ROUMAYNE LOPES; CAMPANINI, EMELINE BONI; SOARES, GABRIELA GUERRERA; CAMPOS, LESLIE CAMELO; LAPREGA, PEDRO MENDES; DA COSTA, ANDREA SOARES; FREIRE, CAIO CESAR DE MELO; PITONDO-SILVA, ANDRE; CERDEIRA, LOUISE TEIXEIRA; et al. Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil. FRONTIERS IN MEDICINE, v. 9, p. 20-pg., 2022-07-28. (18/24213-7, 21/08423-4, 20/11964-4, 18/20697-0, 21/00425-8)