Surveillance and molecular epidemiology of human Bocaviruses associated with acute gastroenteritis

• Grant number: 20/14786-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2021
• End date: March 31, 2024
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Adriana Luchs
• Grantee: Adriana Luchs
• Host Institution: Instituto Adolfo Lutz (IAL). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Antonio Charlys da Costa ; HUGO REIS RESQUE ; Luana da Silva Soares Farias ; Marcelle Figueira Marques da Silva Sales
• Associated scholarship(s): 21/09064-8 - Surveillance and molecular epidemiology of human bocaviruses associated with acute gastroenteritis, BP.TT

Abstract

Human Bocaparvovirus (HBoV) was described in 2005, during a study aimed at investigating the presence of etiological agents involved in respiratory infections in hospitalized children in Sweden. There are 4 HBoV genotypes identified so far. HBoV-1 is commonly associated with respiratory infections, while HBoV-2, HBoV-3 and HBoV-4 are often detected in faecal specimens. HBoV has a global distribution and epidemiological studies worldwide confirm its importance as an etiological agent associated with both diarrheal disease and respiratory disease. The worldwide prevalence of HBoV ranges from 1% to 56.8% in respiratory secretion samples and from 1.3% to 63% in faecal specimens. Co-infections involving HBoV and other respiratory and enteric viral agents are common. In Brazil, the first detection of HBoV occurred in 2007 in fecal samples from children with acute gastroenteritis. However, little is known about the incidence of HBoV both in association with diarrheal disease and respiratory conditions in the country. An increase in the frequency of emerging viruses associated with diarrheal and respiratory conditions has been observed in recent years, leading to the need to improve the accuracy of epidemiological and laboratory surveillance for these agents. This project aims to describe the occurrence of HBoV in respiratory secretions and faecal samples from outpatients and to identify the HBoV genotypes associated with their diseases, aiming to establish the role and impact of HBoV as causative agents of respiratory and diarrheal diseases. in Brazil. It also proposes to determine the frequency and spatiotemporal distribution of HBoV in Brazil in patients with acute gastroenteitis. This study will be divided into two phases. Phase 1 aims to recognize the causal relationship between HBoV genotypes and associated respiratory / gastroenteric injury. In this phase will be analyzed samples of respiratory secretion and fecal specimen (paired) of patients of both sexes in different age groups from January to December 2021, attended at outpatient clinics of the metropolitan region of Belém, Pará. There will be collection of patients presenting symptoms suggestive of respiratory infection and/or acute gastroenteritis, as well as asymptomatic individuals for these diseases. Phase 2 aims to define the prevalence of HBoV genotypes in Brazil in relation to space and time. This phase will comprise a retrospective study conducted with fecal samples from national rotavirus surveillance collected between 1998 and 2018. The samples included in the study will be tested for HBoV by real-time PCR (qPCR) targeting the gene coding for the NS1 protein. Genotyping of HBoV positive samples will be performed by conventional PCR targeting VP1/2 genes, followed by gene sequencing. Representative HBoV positive samples (~ 10%) from the entire study period will be selected for phylogenetic and evolutionary analysis. This is a pioneering study in the country. The present study will increase the epidemiological knowledge of infections caused by HBoV, as well as their genetic diversity. Data from this study will also contribute to a better understanding of the role of HBoV in gastroenteritis in the country following the introduction of rotavirus vaccination, as well as historical samples collected prior to its description in 2005. By providing HBoV genomic sequences in publicly available nucleotide sequence annotation databases, it is hoped that the present investigation may also assist in the strategic planning of disease control in the face of an unexpected emergency, as well as in the ability to establish more consistent epidemiological connections. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS, ENDRYA DO SOCORRO FORO; RIBEIRO, GEOVANI DE OLIVEIRA; VILLANOVA, FABIOLA; MILAGRES, FLAVIO AUGUSTO DE PADUA; BRUSTULIN, RAFAEL; ARAUJO, EMERSON LUIZ LIMA; PANDEY, RAMENDRA PATI; RAJ, V. SAMUEL; DENG, XUTAO; DELWART, ERIC; et al. Composition of Eukaryotic Viruses and Bacteriophages in Individuals with Acute Gastroenteritis. Viruses-Basel, v. 13, n. 12, DEC 2021. (20/14786-0, 15/12944-9, 17/00021-9)

FURUSATO, ISABELLA NAOMI; FIGUEIREDO, KETLYN BOLSACHINI; DE CARVALHO, ANA CAROLINA SOUZA RAMOS; FERREIRA, CAMILA SANTOS DA SILVA; TAKAHASHI, JULIANA POSSATTO FERNANDES; KIMURA, LIDIA MIDORI; ALEIXO, CAMILA SIQUEIRA; DE BRITO, ODILIA PEREIRA; LUCHS, ADRIANA; CUNHA, MARIANA SEQUETIN; et al. Detection of herpesviruses in neotropical primates from Sao Paulo, Brazil. Brazilian Journal of Microbiology, v. N/A, p. 9-pg., 2023-09-09. (17/50333-7, 20/14786-0)

MEDEIROS, ROBERTA SALZONE; FRANCA, YASMIN; VIANA, ELLEN; DE AZEVEDO, LAIS SAMPAIO; GUIDUCCI, RAQUEL; DE LIMA NETO, DANIEL FERREIRA; DA COSTA, ANTONIO CHARLYS; LUCHS, ADRIANA. Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone. Viruses-Basel, v. 15, n. 3, p. 34-pg., 2023-03-01. (20/02469-0, 15/12944-9, 20/14786-0, 20/11182-6)

DE AZEVEDO, LAIS SAMPAIO; FRANCA, YASMIN; VIANA, ELLEN; MEDEIROS, ROBERTA SALZONE; MORILLO, SIMONE GUADAGNUCCI; GUIDUCCI, RAQUEL; RIBEIRO, CIBELE DANIEL; VIEIRA, HELOISA ROSA; BARRIO-NUEVO, KAROLINA MORALES; CUNHA, MARIANA SEQUETIN; et al. Lack of molecular evidence of fecal-borne viruses in capybaras from Sao Paulo state, Brazil, 2018-2020: a minor public health issue. Brazilian Journal of Microbiology, v. N/A, p. 9-pg., 2022-11-07. (20/14786-0, 20/02469-0, 15/12944-9, 20/11182-6, 19/21706-5)

CUNHA, LIA; LUCHS, ADRIANA; AZEVEDO, LAIS S. S.; SILVA, VANESSA C. M.; LEMOS, MARCILIO F. F.; COSTA, ANTONIO C. C.; COMPRI, ADRIANA P. P.; FRANCA, YASMIN; VIANA, ELLEN; MALTA, FERNANDA; et al. Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil. Viruses-Basel, v. 15, n. 2, p. 13-pg., 2023-02-01. (20/02469-0, 15/12944-9, 20/14786-0, 20/11182-6)

AZEVEDO, LAIS SAMPAIO; COSTA, FERNANDA FARIA; GHANI, MONIQUE BEERENS ABDUL; VIANA, ELLEN; FRANCA, YASMIN; MEDEIROS, ROBERTA SALZONE; GUIDUCCI, RAQUEL; MORILLO, SIMONE GUADAGNUCCI; PRIMO, DIELI; LOPES, RICARDO DUARTE; et al. Full genotype characterization of Brazilian canine G3P[3] strains during a 10-year survey (2012-2021) of rotavirus infection in domestic dogs and cats. ARCHIVES OF VIROLOGY, v. 168, n. 7, p. 15-pg., 2023-07-01. (20/02469-0, 15/12944-9, 20/14786-0, 20/11182-6)

FRANCA, YASMIN; MEDEIROS, ROBERTA SALZONE; VIANA, ELLEN; DE AZEVEDO, LAIS SAMPAIO; GUIDUCCI, RAQUEL; DA COSTA, ANTONIO CHARLYS; LUCHS, ADRIANA. Genetic diversity and evolution of G12P[6] DS-1-like and G12P[9] AU-1-like Rotavirus strains in Brazil. FUNCTIONAL & INTEGRATIVE GENOMICS, v. 24, n. 3, p. 40-pg., 2024-06-01. (20/11182-6, 17/50333-7, 20/02469-0, 20/14786-0, 22/02529-8, 21/09064-8, 15/12944-9)

DE AZEVEDO, LAIS SAMPAIO; SILVA, VANESSA CRISTINA MARTINS; GUIDUCCI, RAQUEL; GUADAGNUCCI, SIMONE; COSTA, FERNANDA FARIA; GHANI, MONIQUE BEERENS ABDUL; LOPES, RICARDO DUARTE; DA COSTA, ANTONIO CHARLYS; CUNHA, LIA; LEMOS, MARCILIO FIGUEREDO; et al. Emerging zooanthroponotic risks: Detection of the human norovirus GII.4 Sydney[P31] strain in a domestic dog in Brazil. Acta Tropica, v. 260, p. 6-pg., 2024-12-01. (20/14786-0, 17/50333-7, 15/12944-9)

ADRIANA LUCHS; LAIS SAMPAIO DE AZEVEDO; ELLEN VIANA DE SOUZA; ROBERTA SALZONE MEDEIROS; YASMIN FRANÇA VIANA PIRES DE SOUZA; DALANE LOUDAL FLORENTINO TEIXEIRA; THIAGO FRANCO DE OLIVEIRA CARNEIRO; GABRIELA MARIA FERNANDES DE ALENCAR; FERNANDA LÚCIA DE SOUSA LEITE MORAIS; DIANA DE FÁTIMA ALVES PINTO; et al. Coxsackievirus A6 strains causing an outbreak of hand-foot-and-mouth disease in Northeastern Brazil in 2018. Revista do Instituto de Medicina Tropical de São Paulo, v. 64, 2022-02-21. (20/11182-6, 19/21706-5, 20/14786-0, 17/00021-9, 20/02469-0)

MATOS, AMANDA DE OLIVEIRA; ARAUJO, MAISA; PAULINO, JORDANA; FRANCO, FERNANDA CRAVEIRO; LUCHS, ADRIANA; SALES-CAMPOS, HELIOSWILTON; FIACCADORI, FABIOLA; SOUZA, MENIRA; SILVA-SALES, MARCELLE. Mutations in the main antigenic sites of VP7 and VP8*from G3P[8] rotavirus a strains circulating in Brazil may impact immune evasion to rotavirus vaccination. Brazilian Journal of Microbiology, v. N/A, p. 12-pg., 2024-11-07. (20/14786-0, 15/12944-9)