Antimicrobial peptides: expanding the horizons for animal production performance optimization

• Grant number: 21/06706-9
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• Start date: February 01, 2022
• End date: January 31, 2027
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Eduardo Festozo Vicente
• Grantee: Eduardo Festozo Vicente
• Host Institution: Faculdade de Ciências e Engenharia. Universidade Estadual Paulista (UNESP). Campus de Tupã. Tupã , SP, Brazil
• Associated researchers: Camila Pires Cremasco Gabriel ; Luciano Hauschild ; Marlus Chorilli ; Priscilla Ayleen Bustos Mac-Lean Megna
• Associated research grant: 16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition, AP.JP
• Associated research grant(s): 22/05319-4 - Multi-user equipment approved in grant 2021/06706-9: Mass Spectrometer + UHPLC, AP.EMU
• Associated scholarship(s): 24/23197-9 - Analysis of the supplementation of the microencapsulated antimicrobial peptide Ctx(Ile²¹)-Ha on performance and intestinal health of swines, BP.MS; 24/20054-2 - In vitro analysis of the addition of antimicrobial peptide analogues Ctx(Ile21)-Ha on rumen fermentation parameters, BP.IC; 23/01834-4 - Evaluation of the Ctx(Ile21)-Ha antimicrobial peptide supplementation in laying hens and its effect on egg quality, BP.MS; + associated scholarships 22/09379-1 - Synthesis and obtaining of Ctx(Ile21)-Ha antimicrobial peptide analogues for evaluation of its application as a zootechnical additive in broilers, BP.MS; 22/02808-4 - Evaluation of protease inhibitory properties and biological activities from Allexis cauliflora's cyclotides for application in animal nutrition, BP.PD; 22/01544-3 - C-terminal truncated sequences of Ctx(Ile21)-Ha antimicrobial peptide: decreasing molecular mass to increase biological activity, BP.IC - associated scholarships

Abstract

Antimicrobial resistance is a worldwide threat and demands urgent actions in different re-search areas for development of strategies to proper combat it. Some studies suggest that, until 2050, uncontrolled diseases caused by microorganism can lead 10 million people to death, which will overcome the deaths caused by cancer at the same period. In this way, antimicrobi-al peptides (AMP) are small natural proteins present in all live organisms, belonging to the in-nate immune system. These molecules are very versatile, exhibiting a broad-spectrum of bio-logical activities to combat viruses, fungi and mainly bacteria. In particular, the peptide Ctx(Ile21)-Ha has been extensively studied by our research group and has been shown to be a promising molecule alternative for a new and natural zootechnical additive for replacement of conventional antibiotics used in animal production. Therefore, the objective of this project is expand the application fields of the Ctx(Ile21)-Ha antimicrobial peptide, in order to confirm the potential of this molecule and its analogues as feed additives in animal production. For this, more potent Ctx(Ile21)-Ha peptide analogues will be proposed for raise the biological ac-tivities' effectiveness against the most pathogenic bacteria in animal production (mainly Sal-monella sp. and Escherichia coli). The selected peptides will be microencapsulated and tested in monogastric animals and ruminal liquid from beef cattle animals, for improvement of the performance and production parameters. In parallel, an evaluation of the egg quality promoted by the peptide will be carried out and, for dairy cows application of the Ctx(Ile21)-Ha AMP or analogues against mastitis infection will be conducted. Finally, more potent Ctx(Ile21)-Ha AMP analogues will be applied in food preservation for increase the shelf life of perishable fruits. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ETEME, OLIVIER NDOGO; ZONDEGOUMBA, ERNESTINE NKWENGOUA; TADAYOZZI, YASMIN SAEGUSA; SERAFIM, JESSICA APARECIDA; LEITE, WENDELL QUEIROZ; SEVERINO, MARIANE DE FREITAS GENARI; VICENTE, EDUARDO FESTOZO. Methods for extraction, isolation and sequencing of cyclotides and others cyclic peptides with anti-helminthic activities: An overview. Parasitology International, v. 98, p. 10-pg., 2023-10-04. (21/06706-9, 22/02808-4)

DE SOUZA, ANGELA VACARO; FAVARO, VITORIA FERREIRA DA SILVA; DE MELLO, JESSICA MARQUES; CANATO, VINICIUS; SARTORI, DIOGO DE LUCCA; PUTTI, FERNANDO FERRARI; TADAYOZZI, YASMIN SAEGUSA; SALGADO, DOUGLAS D'ALESSANDRO. Prediction of Bioactive Compounds and Antioxidant Activity in Bananas during Ripening Using Non-Destructive Parameters as Input Data. FOODS, v. 13, n. 14, p. 14-pg., 2024-07-01. (21/06706-9, 20/01711-1, 21/08901-3, 20/14166-1)