Cellular and electrophysiological changes in the hippocampus caused by neurodegenerative processes related to neonatal anoxia and temporal lobe epilepsy

• Grant number: 20/11667-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2022
• End date: October 31, 2024
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Alexandre Hiroaki Kihara
• Grantee: Alexandre Hiroaki Kihara
• Host Institution: Centro de Matemática, Computação e Cognição (CMCC). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
• City of the host institution: Santo André
• Associated researchers: Érika Reime Kinjo ; Silvia Honda Takada

Abstract

Neurodegenerative diseases and neuronal disorders cause the loss of specific neuronal subtypes, thus contributing to the clinical changes observed in patients. Neonatal anoxia and temporal lobe epilepsy are important public health problems worldwide, since in addition to the mortality rate, they can cause permanent sequelae, probably related to the imbalance between excitatory and inhibitory neurons. The impact of neonatal anoxia and temporal lobe epilepsy on the balance of excitatory and inhibitory neurons is poorly understood. Also, cell loss in neuronal disorders has been linked to oxidative stress and neuroinflammation. In this project, we intend to evaluate possible changes in interneuron populations in animals submitted to models of neonatal anoxia and temporal lobe epilepsy. Among other approaches, we will assess the impact of NOX2, an essential enzyme in the production of reactive oxygen species (ROS), related to both microglia activation and neuronal plasticity. Electrophysiological analyzes will be performed using records using HD-MEA BioCAM 4096 on hippocampus slices, in addition to electrophysiological records performed in vivo. This project has the support of a network of collaborators involving UCLA, USP, and Humboldt University at Berlin. With this, we intend to contribute to future therapeutic proposals related to cognitive deficits related to neonatal anoxia and temporal lobe epilepsy. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, CAYO; PONGILIO, RENAN PASCHOALINO; MOVIO, MARILIA INES; HIGA, GUILHERME SHIGUETO VILAR; RESENDE, RODRIGO RIBEIRO; JIANG, JIANXIONG; KINJO, ERIKA REIME; KIHARA, ALEXANDRE HIROAKI. Distinct Cell-specific Roles of NOX2 and MyD88 in Epileptogenesis. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 10, p. 8-pg., 2022-07-04. (20/11667-0, 19/17892-8)

FERRAZ, MARIANA SACRINI AYRES; MUOTRI, ALYSSON R.; KIHARA, ALEXANDRE HIROAKI. Deciphering Complexity in Human Brain Organoids via a Novel Hurst Exponent Estimation. Physical Review Letters, v. 135, n. 10, p. 8-pg., 2025-09-04. (19/15024-9, 20/11667-0, 17/26439-0, 22/14124-2)

MOVIO, MARILIA INES; DE LIMA-VASCONCELLOS, THEO HENRIQUE; DOS SANTOS, GABRIELI BOVI; ECHEVERRY, MARCELA BERMUDEZ; COLOMBO, ELISABETTA; MATTOS, LEONARDO S.; RESENDE, RODRIGO RIBEIRO; KIHARA, ALEXANDRE HIROAKI. Retinal organoids from human-induced pluripotent stem cells: From studying retinal dystrophies to early diagnosis of Alzheimer?s and Parkinson?s disease. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 144, p. 10-pg., 2023-03-17. (21/14227-3, 21/03711-1, 19/17892-8, 20/02035-0, 20/11667-0, 21/11969-9, 17/26388-6)

DE OLIVEIRA, LUCAS FELIPE; MENDES FILHO, DANIEL; MARQUES, BRUNO LEMES; MACIEL, GIOVANA FIGUEIREDO; PARREIRA, RICARDO CAMBRAIA; NETO, JOSE RODRIGUES DO CARMO; DA SILVA, PRISCILLA ELIAS FERREIRA; GUERRA, RHANOICA OLIVEIRA; DA SILVA, MARCOS VINICIUS; SANTIAGO, HELTON DA COSTA; et al. Organoids as a novel tool in modelling infectious diseases. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 144, p. 10-pg., 2023-03-17. (18/07366-4, 20/11667-0)

SANTOS, ANDERSON K.; SCALZO, SERGIO; DE SOUZA, RAYSA T. V.; SANTANA, PEDRO H. G.; MARQUES, BRUNO L.; OLIVEIRA, LUCAS F.; FILHO DANIEL, M.; KIHARA, ALEXANDRE HIROAKI; SANTIAGO, HELTON DA COSTA; PARREIRA, RICARDO C.; et al. Strategic use of organoids and organs-on-chip as biomimetic tools. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 144, p. 8-pg., 2023-03-17. (18/07366-4, 20/11667-0, 12/50880-4)

DA SILVA, VICTOR A.; BOBOTIS, BIANCA C.; CORREIA, FELIPE F.; LIMA-VASCONCELLOS, THEO H.; CHIARANTIN, GABRIELLY M. D.; DE LA VEGA, LAURA; LOMBELLO, CHRISTIANE B.; WILLERTH, STEPHANIE M.; MALMONGE, SONIA M.; PASCHON, VERA; et al. The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 17, p. 25-pg., 2023-09-01. (17/12112-9, 18/06316-3, 18/19300-8, 19/17892-8, 19/18921-1, 20/02035-0, 20/11667-0, 21/11969-9, 19/24406-2)

DE LIMA-VASCONCELLOS, THEO HENRIQUE; BOVI DOS SANTOS, GABRIELI; MOVIO, MARILIA INES; DONNICI, GIOVANNA KLEMENC; BADIN, GABRIELA MARIA; DE ARAUJO, DANIELE RIBEIRO; KIHARA, ALEXANDRE HIROAKI. Neuroprotection provided by polyphenols and flavonoids in photoreceptor degenerative diseases. NEURAL REGENERATION RESEARCH, v. 21, n. 3, p. 15-pg., 2026-03-01. (24/00828-3, 24/10858-7, 20/11667-0, 21/11969-9, 21/14227-3)

MARINHO, LUCIANA SIMOES RAFAGNIN; CHIARANTIN, GABRIELLY MARIA DENADAI; IKEBARA, JULIANE MIDORI; CARDOSO, DEBORA STERZECK; DE LIMA-VASCONCELLOS, THEO HENRIQUE; HIGA, GUILHERME SHIGUETO VILAR; FERRAZ, MARIANA SACRINI AYRES; DE PASQUALE, ROBERTO; TAKADA, SILVIA HONDA; PAPES, FABIO; et al. The impact of antidepressants on human neurodevelopment: Brain organoids as experimental tools. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 144, p. 10-pg., 2023-03-17. (19/17892-8, 20/16268-6, 20/11451-7, 20/02035-0, 21/11969-9, 20/11667-0, 19/15024-9, 16/17329-3)

MARQUES, BRUNO L.; MACIEL, GIOVANA FIGUEIREDO; BRITO JUNIOR, MARCELLO R.; DIAS, LUCAS D.; SCALZO, SERGIO; SANTOS, ANDERSON K.; KIHARA, ALEXANDRE HIROAKI; SANTIAGO, HELTON DA COSTA; PARREIRA, RICARDO C.; BIRBRAIR, ALEXANDER; et al. Regulatory mechanisms of stem cell differentiation: Biotechnological applications for neurogenesis. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 144, p. 9-pg., 2023-03-17. (19/17892-8, 20/11667-0)

IKEBARA, JULIANE MIDORI; JORGE, RENATA SILVA; MARINHO, LUCIANA SIMOES RAFAGNIN; HIGA, GUILHERME SHIGUETO VILAR; ADHIKARI, AVISHEK; REIS, FERNANDO M. C. V.; BORGES, FERNANDO S.; ULRICH, HENNING; TAKADA, SILVIA HONDA; DE PASQUALE, ROBERTO; et al. Hippocampal Interneurons Shape Spatial Coding Alterations in Neurological Disorders. Molecular Neurobiology, v. N/A, p. 24-pg., 2025-05-20. (20/16268-6, 20/11667-0, 22/00850-3, 22/09277-4, 18/07366-4)

ZHOU, ZHI DONG; KIHARA, ALEXANDRE HIROAKI. Neurodegenerative Diseases: Molecular Mechanisms and Therapies 2nd Edition. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 25, n. 21, p. 3-pg., 2024-11-01. (20/11667-0)

KIHARA, ALEXANDRE HIROAKI. GABA Signaling in Health and Disease in the Nervous System. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 25, n. 20, p. 3-pg., 2024-10-01. (20/11667-0)

MOVIO, MARILIA INES; DE ALMEIDA, GIOVANA WANER CARNEIRO; MARTINES, ISABELLA DAS GRACAS LOPES; BARROS DE LIMA, GILMARA; SASAKI, SERGIO DAISHI; KIHARA, ALEXANDRE HIROAKI; POOLE, EMMA; NEVELS, MICHAEL; DA SILVA, MARIA CRISTINA CARLAN. SARS-CoV-2 ORF8 as a Modulator of Cytokine Induction: Evidence and Search for Molecular Mechanisms. Viruses-Basel, v. 16, n. 1, p. 15-pg., 2024-01-01. (20/11667-0)