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Evaluation of the immune response, metabolism and chemosensitivity of chickens after in ovo microbiota alteration


In the present study, we wil evaluete the effects of inoculation with probiotic or antibiotic on day 16 of incubation on metabolism, chemosensitivity (hypoxic and hypercapnic response), immune response to challenge with lipopolysaccharide (LPS) considering the microbiota-intestine-brain axis, and chick microbiota juveniles. To this end, fertile eggs from Cobb® lineage breeders will be submitted to the following treatments: Control group - eggs will be incubated and no probiotics/antibiotics will be administered in ovo; Group 1 - eggs will be applied vehicle in the amniotic cavity on the 16th day of incubation; Group 2 - eggs will be inoculated on the 16th embryonic day with a commercial probiotic in the amniotic cavity; Group 3 - eggs will be inoculated on the 16th embryonic day with an antibiotic in the amniotic cavity. After hatching, the chicks will be sexed and the following parameters will be evaluated: hatchability, ventilation, body temperature; O2 consumption, LPS- challenge, and metagenomics of the intestinal microbiota of juvenile chicks. Data will be evaluated for compliance with the assumptions of homogeneity of variances and normality of student errors. Then they will be submitted to analysis of variance by the GLM procedure (General Linear Models) of the SAS® program and in case of significant difference, the means will be compared by Tukey test at 5% probability. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PATRONE, LUIS GUSTAVO A.; ROCHA, ALINE C. G.; BERNARDES-RIBEIRO, MARIANA; LOPES-DA-COSTA, GLEYCE; MACARI, MARCOS; BICEGO, KENIA C.; GARGAGLIONI, LUCIANE H.. Heat stress affects breathing and metabolism of chicks incubated at high temperature. Respiratory Physiology & Neurobiology, v. 314, p. 6-pg., . (20/01702-2, 21/13370-7, 19/14646-6)

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