Effect of incubation temperature on chick metabolism, thermoregulation and chemosensitivity

• Grant number: 19/09469-8
• Support type: Regular Research Grants
• Duration: August 01, 2019 - July 31, 2021
• Field of knowledge: Agronomical Sciences - Animal Husbandry
• Principal Investigator: Luciane Helena Gargaglioni Batalhão
• Grantee: Luciane Helena Gargaglioni Batalhão
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The first third of the incubation is determinant for the embryonic development and the temperature considered one of the most important factors, since small fluctuations of this parameter can affect the quality and the survival rate of the embryos. The goal of this proposal will be to investigate the effects of thermal manipulation during incubation on metabolism, body temperature, ventilatory responses to hypoxia and hypercapnia and neuronal activation in newly hatched chicks (2-3 days) and close to the age of slaughter. Fertile eggs of Cobb® lineage matrices, classified according to weight, from matrices between the ages of 43 to 46 weeks of age from commercial hatchery will be incubated according to the following treatments: Control group - the eggs will be incubated under thermoneutral conditions (37.5 ° C); Group 1 - the eggs will be incubated with temperature above the thermoneutral (39 ° C) for 6 hours, for 5 days (0 - 120 hours of incubation); Group 2 - the eggs will be incubated with temperature below the thermoneutral (36 ° C) for 6 hours, for 5 days (0 - 120 hours incubation). After hatching, the animals will be kept at thermoneutral temperature and the following parameters will be evaluated: hatchability; size of organs; chick quality body temperature; O2 consumption and CO2 production at baseline, and after exposure to hypoxia (7% O2) or hypercapnia (7% CO2) and heat stress (32° C); and c-Fos expression. The data will be evaluated regarding the assumption of the homogeneity of variances and normality of the errors studied. Next, they will be submitted to analysis of variance by the General Linear Models (GLM) procedure of the SAS® program and in case of significant difference, the means will be compared by the Tukey test at 5% probability. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARGAGLIONI, LUCIANE H.; MARQUES, DANUZIA A.; PATRONE, LUIS GUSTAVO A. Sex differences in breathing. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 238, DEC 2019. Web of Science Citations: 0.