Effect of incubation temperature on chick metabolism, thermoregulation and chemosensitivity

• Grant number: 19/09469-8
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2019
• End date: September 30, 2021
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Luciane Helena Gargaglioni Batalhão
• Grantee: Luciane Helena Gargaglioni Batalhão
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The first third of the incubation is determinant for the embryonic development and the temperature considered one of the most important factors, since small fluctuations of this parameter can affect the quality and the survival rate of the embryos. The goal of this proposal will be to investigate the effects of thermal manipulation during incubation on metabolism, body temperature, ventilatory responses to hypoxia and hypercapnia and neuronal activation in newly hatched chicks (2-3 days) and close to the age of slaughter. Fertile eggs of Cobb® lineage matrices, classified according to weight, from matrices between the ages of 43 to 46 weeks of age from commercial hatchery will be incubated according to the following treatments: Control group - the eggs will be incubated under thermoneutral conditions (37.5 ° C); Group 1 - the eggs will be incubated with temperature above the thermoneutral (39 ° C) for 6 hours, for 5 days (0 - 120 hours of incubation); Group 2 - the eggs will be incubated with temperature below the thermoneutral (36 ° C) for 6 hours, for 5 days (0 - 120 hours incubation). After hatching, the animals will be kept at thermoneutral temperature and the following parameters will be evaluated: hatchability; size of organs; chick quality body temperature; O2 consumption and CO2 production at baseline, and after exposure to hypoxia (7% O2) or hypercapnia (7% CO2) and heat stress (32° C); and c-Fos expression. The data will be evaluated regarding the assumption of the homogeneity of variances and normality of the errors studied. Next, they will be submitted to analysis of variance by the General Linear Models (GLM) procedure of the SAS® program and in case of significant difference, the means will be compared by the Tukey test at 5% probability. (AU)

Scientific publications (10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, ALINE C. G.; CRISTINA-SILVA, CAROLINE; TAXINI, CAMILA L.; DA COSTA SILVA, KAOMA STEPHANI; LIMA, VIRGINIA T. M.; MACARI, MARCOS; BICEGO, KENIA C.; SZAWKA, RAPHAEL E.; GARGAGLIONI, LUCIANE H.. mbryonic Thermal Manipulation Affects Ventilation, Metabolism, Thermal Control and Central Dopamine in Newly Hatched and Juvenile Chick. FRONTIERS IN PHYSIOLOGY, v. 12, JUN 17 2021. (19/09469-8)

RIPAMONTE, GABRIEL C.; GARGAGLIONI, LUCIANE H.. Functional role for preoptic CB1 receptors in breathing and thermal control. Neuroscience Letters, v. 732, JUL 27 2020. (15/04849-6, 19/09469-8)

MARQUES, DANUZIA A.; PATRONE, LUIS GUSTAVO A.; SCARPELLINI, CAROLINA S.; BICEGO, KENIA C.; SZAWKA, RAPHAEL E.; GARGAGLIONI, LUCIANE H.. The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats. Journal of Endocrinology, v. 247, n. 1, p. 14-pg., 2020-10-01. (16/04276-9, 19/09469-8)

TAXINI, CAMILA L.; MARQUES, DANUZIA A.; BICEGO, KENIA C.; GARGAGLIONI, LUCIANE H.. A5 noradrenergic neurons and breathing control in neonate rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, v. 473, n. 6, APR 2021. (19/09469-8)

MARQUES, DANUZIA A.; GARGAGLIONI, LUCIANE H.; JOSEPH, VINCENT; BRETZNER, FREDERIC; BICEGO, KENIA C.; FOURNIER, STEPHANIE; KINKEAD, RICHARD. Impact of ovariectomy and CO2 inhalation on microglia morphology in select brainstem and hypothalamic areas regulating breathing in female rats. Brain Research, v. 1756, APR 1 2021. (19/09469-8, 16/21730-5)

GARGAGLIONI, LUCIANE H.; MARQUES, DANUZIA A.; PATRONE, LUIS GUSTAVO A.. Sex differences in breathing. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 238, DEC 2019. (16/04276-9, 19/09469-8, 16/24577-3, 17/05318-0)

LONGHINI, LEONARDO S.; ZENA, LUCAS A.; POLYMEROPOULOS, ELIAS T.; ROCHA, ALINE C. G.; LEANDRO, GABRIELA DA SILVA; PRADO, CYNTHIA P. A.; BICEGO, KENIA C.; GARGAGLIONI, LUCIANE H.. Thermal Acclimation to the Highest Natural Ambient Temperature Compromises Physiological Performance in Tadpoles of a Stream-Breeding Savanna Tree Frog. FRONTIERS IN PHYSIOLOGY, v. 12, p. 15-pg., 2021-10-08. (18/01899-0, 19/09469-8)

GARGAGLIONI, LUCIANE H.; MARQUES, DANUZIA A.. Reply to Jakovac: Sex differences in COVID-19 course and outcome: progesterone should not be neglected. Journal of Applied Physiology, v. 129, n. 5, p. 1009-1010, NOV 2020. (19/09469-8)

FONSECA, ELISA M.; VICENTE, MARIANE C.; FOURNIER, STEPHANIE; KINKEAD, RICHARD; BICEGO, KENIA C.; GARGAGLIONI, LUCIANE H.. Influence of light/dark cycle and orexins on breathing control in green iguanas (Iguana iguana). SCIENTIFIC REPORTS, v. 10, n. 1, DEC 16 2020. (19/09469-8)

FONSECA, ELISA M.; NORONHA-DE-SOUZA, CAROLINA R.; BICEGO, KENIA C.; BRANCO, LUIZ G. S.; GARGAGLIONI, LUCIANE H.. 5-HT neurons of the medullary raphe contribute to respiratory control in toads. Respiratory Physiology & Neurobiology, v. 293, NOV 2021. (19/09469-8, 16/17681-9)