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Exposure to hypoxia during incubation and its influence on thermal preference and metabolic curve of broiler chicks during the first week post-hatching

Grant number: 11/07509-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2011
Effective date (End): February 28, 2014
Field of knowledge:Agronomical Sciences - Animal Husbandry
Principal Investigator:Kênia Cardoso Bícego
Grantee:Paula Andrea Toro Velásquez
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):12/11565-6 - Effect of hypoxia during incubation on the O2 and CO2 chemosensitivity of one day chick, BE.EP.DR


In this project we will investigate the influence of hypoxia at different periods of incubation on the embryonic development, on the expression of heat shock protein Hsp70 and the concentrations of T3 and T4 at the end and after incubation and on the thermal preference and metabolic curve in the first days post-hatching of broiler chickens. For this, two experiments will be performed. In the first experiment will be used 210 fertile eggs of Cobb-500 ® distributed in a completely randomized design with three treatments: normoxia (N, 21% O2), hypoxia initial (HI, 15% O2 during the first week of incubation) and hypoxia late (HT, 15% O2 during the last week of incubation). On 19th incubation day (DE19) weighed the embryo and some organs (liver, brain and heart), which will be frozen for later analysis of gene transcription and expression of heat shock protein Hsp70. DE19 also be measured in the plasma concentration of T3 and T4. After the hatching will be evaluated: thermal preference of animals in the first week, the curve metabolic (effect of temperature on metabolic rate) between the 7th and 10th days; gene transcription and protein expression of Hsp70 (liver, heart and brain) and plasma T3 and T4 on day 07 post-hatching. From the results of the first experiment, the second will be used in 1200 fertile eggs of Cobb-500 ® distributed in a completely randomized design with two treatments and N HI or HT. We will evaluate the following parameters in the hatchin: eclodobilidade, chick quality, weight and hatch window. The data will be subjected to analysis of variance by the General Linear Model (GLM) of SAS ® (SAS Institute, 2002) and in case of a statistically significant difference (p <0.05), averages are compared by Tukey test. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DO AMARAL-SILVA, LARA; SCARPELLINI, CAROLINA DA S.; TORO-VELASQUEZ, PAULA ANDREA; FERNANDES, MARCIA H. M. R.; GARGAGLIONI, LUCIANE H.; BICEGO, KENIA C. Hypoxia during embryonic development increases energy metabolism in normoxic juvenile chicks. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 207, p. 93-99, MAY 2017. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VELÁSQUEZ, Paula Andrea Toro. Alterações metabólicas e morfométricas induzidas por hipóxia em Gallus gallus. 2014. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

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