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Effect of hypoxia during late phase of incubation on post-hatching lung structures in broiler

Grant number: 14/01861-2
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Animal Husbandry
Principal Investigator:Kênia Cardoso Bícego
Grantee:Lara do Amaral Silva
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


There is evidence of the effects of hypoxia during different phases of incubation on the development of organs and energetic metabolism of chicken hatchlings. However, no study investigated the effect of hypoxia during late phase of incubation in the lung morphology of chicks. Thus, we will use stereology technique to verify the effect of hypoxia (15%O2) on lung structure of hatchlings and 10 days old chicks. To this end, fertile eggs (Cobb 500 strain) will be incubated in normoxia during entire incubation (Nx) or in hypoxia in the last week of incubation.(HxL). Hatchlings and 10 days old chicks will be euthanized, lungs fixed and lung volume determined using Cavalieri technique. Cubes of 2/2mm in resin will be cut for light and transmission electron microscopy (sequential cuts in crescent angles). Following images it will be possible to determine volume of structures and quantify barrier and surface for gas exchanges.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DO AMARAL-SILVA, LARA; LAMBERTZ, MARKUS; ZARA, FERNANDO JOSE; KLEIN, WILFRIED; GARGAGLIONI, LUCIANE HELENA; BICEGO, KENIA CARDOSO. Parabronchial remodeling in chicks in response to embryonic hypoxia. Journal of Experimental Biology, v. 222, n. 10 MAY 2019. Web of Science Citations: 0.
DO AMARAL-SILVA, LARA; SCARPELLINI, CAROLINA DA S.; TORO-VELASQUEZ, PAULA ANDREA; FERNANDES, MARCIA H. M. R.; GARGAGLIONI, LUCIANE H.; BICEGO, KENIA C. Hypoxia during embryonic development increases energy metabolism in normoxic juvenile chicks. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 207, p. 93-99, MAY 2017. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Lara do Amaral. Remodelamento estrutural do tecido parabronquial de pintinhos em resposta à hipóxia durante o desenvolvimento fetal. 2016. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

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