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Responses of fetuses and chicks of eggs from young and old breeders to incubation temperature deviations: glycolytic metabolism and thermogenesis

Grant number: 12/22133-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2013
Effective date (End): February 28, 2014
Field of knowledge:Agronomical Sciences - Animal Husbandry
Principal Investigator:Isabel Cristina Boleli
Grantee:Viviane de Souza Morita
Supervisor abroad: Hendrik van den Brand
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Local de pesquisa : Wageningen University, Netherlands  
Associated to the scholarship:11/18373-2 - Manipulation of temperature of incubation: effects on thermal preference, characteristics of skin, feathers and bones of broilers, BP.DR

Abstract

The present study will investigate the influence of incubation temperature on the glycolytic metabolism and thermogenesis of fetuses and chicks from eggs of two ages broiler breeders. For this, one experiment will be conducted using fertile eggs (Cobb TM) from young and old breeders. Eggs will be divided into three incubators and will be maintained at 37.5°C until 12 days. From the 13th day until to hatching, two incubators will have its temperature altered, one to 36 º C and another for 39 º C. The other incubator will be remained at 37.5 ° C throughout the experimental period. The relative humidity will be maintained at 60% throughout the incubation period on all incubators, and the eggs will be stopped to be turned from 18th day of incubation. Throughout the incubation period will be evaluated the eggshell temperature and heat production by the same. The analyzes of glucose, lactate and plasma uric acid as well as the quantification of glycogen in muscle, liver and yolk sac will be evaluated in fetuses on 18th day of incubation, during the internal and external piping, in the hatching and 18 hours post-hatching. The quality of newly-hatched chicks will be evaluated based on their morphological and morphometric characteristics. The experiment will follow by completely randomized design with the following treatments: two ages breeder broiler (young and old) and three temperatures (36, 37.5 and 39 C). Data will be submitted to analysis of variance by the General Linear Model (GLM) of SAS ® (SAS Institute, 2002) and in the case of a statistically significant difference (p <0.05), the average will be compared by the Tukey test. (AU)