Solutions for emerging diseases in fish farming: diagnosis, vaccines and breeding

• Grant number: 21/11955-8
• Support Opportunities: Research Grants - Science Centers for Development
• Start date: June 01, 2022
• End date: May 31, 2027
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maria José Tavares Ranzani de Paiva
• Grantee: Maria José Tavares Ranzani de Paiva
• Host Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Principal investigators: Diogo Teruo Hashimoto ; Fabiana Pilarski ; Leonardo Tachibana ; Ricardo Luiz Moro de Sousa
• Associated researchers: Antônio Fernando Gervásio Leonardo ; Carlos Massatoshi Ishikawa ; Cláudia Maris Ferreira Mostério ; Daniela Castellani ; Danielle de Carla Dias ; Eduardo Makoto Onaka ; Fernando Stopato da Fonseca ; Izabela Dutra Alvim ; Leonardo Susumu Takahashi ; Rafael Vilhena Reis Neto ; Silvia Helena Seraphin de Godoy ; Vander Bruno dos Santos
• Associated scholarship(s): 25/18579-2 - Rapid kit for disease diagnosis and vaccination assessment of Nile tilapia, BP.MS; 25/21760-0 - Construction of a plasmid containing the MCP gene of the Infection spleen and kidney necrosis virus fused to the CotC gene for vaccines, BP.IC; 25/17782-9 - Development and evaluation of vaccine products for the control of emerging diseases in fish farming, BP.TT; + associated scholarships 25/19190-1 - Analysis of Gene Expression of Immunological Markers in Nile Tilapia (Oreochromis niloticus) Subjected to DNA Vaccination against Francisella orientalis by intramuscular Route, BP.IC; 25/05872-3 - Development of Rapid Diagnostic Kits for Diseases and Evaluation of Vaccination Efficacy in Nile Tilapia, BP.PD; 25/06294-3 - Use of genomic tools for selection of disease-resistant tilapia (Oreochromis niloticus)., BP.TT; 25/04172-8 - Rapid disease diagnosis and vaccination assessment kit for Nile-tilapia., BP.TT; 25/06063-1 - Recombinant strain development of Lactobacillus casei expressing major capsin protein in infection spleen and kidney necrosis virus (ISKNV), BP.MS; 24/20707-6 - Nile tilapia vaccination using Aeromonas hydrophila ghost cells and its effects on genic expression of IFN-gamma and TNF-alpha, BP.IC; 25/01167-3 - Phenotypic and molecular characterization of ISKNV strains: detection, isolation, and viral production, BP.TT; 24/10640-1 - Development of an oral vaccine based on recombinant Bacillus subtilis endospores for tilapia (Oreochromis niloticus) against infection spleen and kidney necrosis virus (ISKNV), BP.DR; 24/15284-9 - Quantification of the Mean Lethal Dose (LD50) of francisella orientalis in Nile Tilapia (Oreochromis niloticus)., BP.IC; 23/00903-2 - Innate immunity of Nile tilapia (Oreochromis niloticus) and the interaction between virus and bacteria: an in vitro co-infeccion model of macrophages by Ranavirus and Aeromonas hydrophila, BP.PD; 23/04314-1 - Vaccines development for Nile tilapia, BP.PD; 23/04794-3 - Nile Tilapia Rapid Disease Diagnosis Kit and Vaccination Assessment Evaluation, BP.TT; 23/01889-3 - Genetic selection for tilapia (Oreochromis niloticus) production resistant to diseases, BP.TT; 23/01942-1 - Genomic selection for production of tilapia (Oreochromis niloticus) resistant to Francisella noatunensis, BP.PD; 22/11636-2 - Solutions to combat emerging diseases in fish farming: Diagnostics, vaccines and genetic selection, BP.TT - associated scholarships

Abstract

Tilapia, Oreochromis ssp, is the second most cultivated fish in the world and the main specie produced in Brazil. The tilapia health is the main concern of the fish culture chain, and it has been significantly affected by emerging diseases caused by Francisella noatunensis subsp. orientalis (FNO) and, more recently, by the Infectious Necrosis of the Spleen and Kidney virus (ISKNV). The Fish Farming Health Center creation is necessary for the sustainable development of fish production in the State of São Paulo, to improve the technological knowledgments about national fish farming health problems. The objective of this Center will be development a diagnostic kits for emerging diseases, vaccines for these diseases and breeding to disease-related of tilapia. The project will be divided into three subprojects: 1) develop rapid diagnostic kits for FNO, and ISKNV detection based on chromatographic immunoassay test with monoclonal antibodies labeled with nanogold. An ELISA test will be developed to detect and quantify the IgM and identify the fish titration antibody after vaccination; 2) develop new vaccines for Nile tilapia against emerging fish farming diseases (Francisella noatunensis subsp. orientalis and the ISKNV virus); 3) genetic improvement for resistant/tolerant to diseases, with the objective of implementing a genetic selection program through genomics for the tilapia production chain. The potential impact of this study is to enable a technological package to boost the tilapia production chain in the aquaculture industry, with food safety, high genetic quality, added value and improvement in aquaculture health. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, BALTASAR F.; RESTREPO-ARANGO, JAIRO A.; SILVA FILHO, MARCELO S.; SOUSA, ELIELMA L.; AGUDELO, JOHN F. G.; MASTROCHIRICO-FILHO, VITO A.; MANSO, SHISLEY C. S.; PEREIRA, CAROLINA S.; DIAS, DANIELLE C.; LEONARDO, ANTONIO F. G.; et al. Genetic selection for resistance to Francisella orientalis shows significant selection response in Nile tilapia. Aquaculture, v. 606, p. 8-pg., 2025-04-21. (21/11955-8, 23/01942-1, 23/01889-3)