Sustainable citriculture through controlled release of antibacterial compounds from microgel-based formulations

• Grant number: 21/10839-4
• Support Opportunities: Research Projects - Thematic Grants
• Start date: October 01, 2022
• End date: September 30, 2027
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Agreement: Netherlands Organisation for Scientific Research (NWO)
• Principal Investigator: Henrique Ferreira
• Grantee: Henrique Ferreira
• Principal researcher abroad: Dirk-Jan Scheffers
• Institution abroad: University of Groningen, Netherlands
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated researchers: Andrij Pich ; Fernando Rogério Pavan ; Franklin Behlau ; Michel Brienzo ; Neidiquele Maria Silveira ; Peter Deuss ; Yvonne van der Meer
• Associated research grant(s): 24/01857-7 - Assessment of biopesticides presence, retention and biodegradation residues in citrus leaves, fruit and soil, AP.R
• Associated scholarship(s): 24/23713-7 - Combination of chitosan with different polysaccharides for the production of a biogel carrier of eugenol with bactericidal, fungicidal, and repellent action., BP.MS; 24/21002-6 - Determination of physicochemical properties of bioplastic/biogel produced with different polysaccharides, BP.TT; 24/14761-8 - Evaluate the anti-X. citri activity of formulations and microgels, BP.TT; + associated scholarships 24/07045-4 - Physicochemical determination of bioplastic/biogel produced with different polysaccharides, BP.TT; 24/03745-1 - Evaluate the anti-X. citri activity of formulations and microgels, BP.TT; 23/12187-0 - Comparison of different polysaccharides in combination with chitosan in the preparation of biogel for controlled release of antibacterials in citrus growing, BP.PD; 23/03002-6 - Chitosan gel formulation, drying profile and influence of its application on gas exchange, BP.MS; 23/06702-9 - Development of a Chitosan Biogel for Carrying Eugenol in Combating Citrus Canker: Scale Production and Field Evaluation, BP.PD; 23/14687-0 - Microgels: validation of the anti-X. citri activity, toxicity, plant protection ability, and plant fixation, BP.PD; 23/11269-2 - Evaluate formulations of chitosan for biogel production and determination of their mechanical properties, BP.TT; 23/03027-9 - Characterization of the role of cohLAB genes in copper resistance in Xanthomonas citri subsp. citri., BP.IC; 23/02969-0 - Evaluation of active compounds against Xanthomonas citri, BP.TT - associated scholarships

Abstract

There is a clear need for sustainable methods to combat agricultural pests. Copper salts are widely used in regular agriculture, but also in organic agriculture. One agricultural area where copper salts are used widely is citriculture, to combat citrus canker, a disease caused by the plant pathogen Xanthomonas citri subsp. citri. The disease is endemic in all major orange producing regions in the world, except Europe. Citriculture generates US$2 billion/year in export revenues in Brazil indicating the size and importance of this industry. This project is aimed at the development of a bio-based, sustainable technology for the protection of citrus trees, as an alternative to copper. Specifically, we will focus on the fabrication of stimuli-responsive microgels that are capable of 1) strong attachment to citrus leaves and 2) controlled release of antibacterial compounds. In collaboration with our societal partner in Brazil, the efficacy of the protection system will be tested in the field. Our research program is based on interdisciplinary research contributing to practical solutions for the production of biomass-derived building blocks for the bio-based plant protection systems and sustainable agriculture of the future. Within this project, the partners will combine previously developed antibacterial compounds that target Xanthomonas (gallates, dihydroxybenzoates, eugenol) with microgels, that offer binding to plant leaves and long-term stability. The microgel carriers for antimicrobial compounds will be synthesized using biopolymer (chitosan) building blocks, to ensure complete degradation under field conditions. The extraction and up-scaling of biopolymer production will be optimized for large-scale microgel synthesis. Controlled release of compounds from the microgel will be achieved through use of environmental triggers (humidity, light, enzymes, etc.) that will allow dosage-controlled, precise, and efficient deactivation of bacteria. Rain simulation within a greenhouse will be used to verify if and how well the microgels/compounds can be fixed on leaves. Thus, microgels will be optimized so that release and antibacterial efficacy is optimal at farming conditions. Also, the toxicity to honeybees and other models will be determined to establish whether the microgels are safe to use. Production of the best performing microgel/antibacterial combination will be scaled up. Subsequently, field tests at our societal partner will be done to determine the efficacy of the microgel/antibacterial to protect against citrus canker in farming conditions in comparison with copper and treatment with non-encapsulated antibacterials. In parallel with the development of the microgels, our partner Santa Clara has the expertise to test for compatibility among active compounds and adjuvants, and to prepare formulations that can be directly and easily evaluated in green-house and field trials. Formulations will be tested for effectiveness using the green-house plant protection test, and the best selected formulations will be subsequently tested in the field. To analyse environmental impact, biodegradation and ecotoxicity of the antibacterial molecules, formulations and microgels will be analyzed through soil analysis for compound retention, and metagenome sequencing to determine what the effects of the various compounds are on the soil microbiome. Life Cycle Assessment and market analysis will be performed to assess the environmental, economic, and social impact of the new technology. We plan a comparative Life Cycle Assessment of the environmental and health impact of formulated product that will be developed within this project and the currently used copper-based formulations. The market analysis will include an analysis of social impacts relevant for stakeholders like governments and farmers. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, JULIA RIBEIRO; LLANOS, JAIBER HUMBERTO RODRIGUEZ; BOTARO, VAGNER; GONCALVES, ADILSON ROBERTO; BRIENZO, MICHEL. Hemicellulose Biomass Degree of Acetylation (Natural Versus Chemical Acetylation) as a Strategy for Based Packaging Materials. BioEnergy Research, v. 17, n. 2, p. 20-pg., 2024-02-27. (17/22401-8, 21/10839-4, 23/06702-9)

MARIN, VITOR RODRIGUES; ZAMUNER, CAIO FELIPE CAVICCHIA; HYPOLITO, GIOVANE BOERNER; FERRAREZI, JULIANO HENRIQUE; ALLEONI, NATALIA; CACCALANO, MARIO NICOLAS; FERREIRA, HENRIQUE; SASS, DAIANE CRISTINA. Antibacterial activity of Cymbopogon species essential oils against Xanthomonas citri and their use in post-harvest treatment for citrus canker management. Letters in Applied Microbiology, v. 77, n. 5, p. 8-pg., 2024-05-14. (21/10839-4)

MARIN, VITOR RODRIGUES; ZAMUNER, CAIO FELIPE CAVICCHIA; DOS SANTOS, EDUARDA ARAUJO; FERREIRA, HENRIQUE; SASS, DAIANE CRISTINA. Essential oils as alternatives in the control of phytopathogens: A systematic review of the last five years. Journal of Essential Oil Bearing Plants, v. 27, n. 4, p. 35-pg., 2024-07-10. (21/10839-4)

MARTINS, JULIA RIBEIRO; LLANOS, JAIBER HUMBERTO RODRIGUEZ; ABE, MATEUS MANABU; COSTA, MICHELLE LEALI; BRIENZO, MICHEL. New blend of renewable bioplastic based on starch and acetylated xylan with high resistance to oil and water vapor. Carbohydrate Research, v. 537, p. 12-pg., 2024-02-27. (17/22401-8, 21/10839-4)

ZAMORA, HERNAN DARIO ZAMORA; FERREIRA, HENRIQUE; PICH, ANDRIJ; BRIENZO, MICHEL. Hemicellulose additive to chitosan-based bioplastic improved the tensile strength and allowed to control the material swelling. POLYMER BULLETIN, v. 81, n. 10, p. 21-pg., 2024-02-05. (17/22401-8, 21/10839-4)

CASTANHEIRA, JULIA DE PAULA; LLANOS, JAIBER HUMBERTO RODRIGUEZ; MARTINS, JULIA RIBEIRO; COSTA, MICHELLE LEALI; BRIENZO, MICHEL. Acetylated xylan from sugarcane bagasse: advancing bioplastic formation with enhanced water resistance. POLYMER BULLETIN, v. N/A, p. 18-pg., 2024-11-30. (24/06991-3, 23/06702-9, 21/10839-4)

ZAMUNER, CAIO FELIPE CAVICCHIA; MARIN, VITOR RODRIGUES; DILARRI, GUILHERME; HYPOLITO, GIOVANE BOERNER; SASS, DAIANE CRISTINA; FERREIRA, HENRIQUE. Oregano essential oil and its main components Thymol and Carvacrol as alternatives to control citrus canker. FRONTIERS IN AGRONOMY, v. 5, p. 11-pg., 2023-03-31. (18/21164-5, 17/50454-9, 21/10839-4, 17/09233-9)