Development and characterization of adhesive system with curcumin anchored to nanosillic on dentin/composite bond strength

• Grant number: 23/00403-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2023
• End date: June 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Fernanda de Carvalho Panzeri
• Grantee: Fernanda de Carvalho Panzeri
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Mário Alexandre Coelho Sinhoreti ; WASHINGTON LUIZ ESTEVES MAGALHAES

Abstract

This study aims to evaluate the efficiency of the incorporation of curcumin (CAN) anchored in nanosilica into an experimental adhesive system to characterize the mechanical and antimicrobials properties of the suggested adhesive protocols, as well as to evaluate the bond strength to dentin, the morphological characteristics of the adhesive interface and mass variation after dentin biomodification. The first step of this study will determine the two concentrations of curcumin to be used. Thus, an experimental adhesive system containing curcumin anchored in nanosilica will be tested at concentrations of 0%,1% 2,5% and 5%. The flexural strength and the antimicrobial potential will be evaluated. The results found will support the choice of the two most adequate concentrations of curcumin to assess the bond strength (BS). Sound human molars will be selected and cavities (6 x 7 x 4 mm) prepared using carbide burs. Half of them will be submitted to cariogenic challenge by microbiological method with a standard strain S. mutans ATCC25175. Subsequently, the teeth will be separated into groups according to the adhesive protocols to which they will be submitted: Control - Commercial adhesive system (Single Bond Universal - 3M ESPE); CAN0% Group - Exp + CAN a 0%; CAN1 Group - Exp + CAN at a certain concentration; CAN2 Group - Exp + CAN at a certain concentration. After restoration with composite resin (Filtek Z350, 3M ESPE), all the samples will be sectioned into sticks, separated and stored in distilled water at 37 ºC for 24 hours, 6 months, and 1 year. After these periods, they will be subjected to the microtensile test (OM100, Odeme) at a speed of 0.75 mm/min. The fractured sticks will be observed in a stereomicroscope for analysis of the fracture pattern (cohesive, adhesive, or mixed) and then, processed and analyzed by scanning electron microscopy (JSM 5410, Sony). The mass variation after biomodification will be analyzed. The quantitative data obtained will be analyzed statistically according to the data normality analysis. (AU)