Development and evaluation of a biomimetic material based on CEMP-1-p1, for remineralization of demineralized tooth enamel.

• Grant number: 22/12445-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2023
• End date: February 28, 2026
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Paulo Nelson Filho
• Grantee: Paulo Nelson Filho
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Carolina Patrícia Aires Garbellini ; Léa Assed Bezerra da Silva ; Maria Vitória Lopes Badra Bentley ; Raquel Assed Bezerra da Silva
• Associated scholarship(s): 23/15273-4 - Development and evaluation of a biomimetic material based on CEMP-1-p1, for remineralization of demineralized tooth enamel., BP.TT

Abstract

Dental caries, which first stage is enamel demineralization, affects 60-90% of school-age children worldwide. Thus, the study of materials and techniques that cause enamel remineralization is relevant in clinical practice. With the discovery of the functional role of enamel-forming proteins involved in the apatite mineralization, biomimetic materials have been proposed for enamel regeneration. The protein "Cementum Protein-1 (CEMP-1)" is a nuclear protein, expressed in cementoblasts, cells of the periodontal ligament and endostal spaces of bones, which promotes cell fixation and differentiation, as well as regulates deposition, composition and the morphology of the hydroxyapatite crystals. Thus, the present project aims to develop a liquid-crystalline gel and cubic phase nanoparticles containing the peptide CEMP-1-p1, for remineralization of demineralized tooth enamel. The in vitro and ex vivo characterization and evaluation of the new biomimetic material will be carried out, verifying the peptide release profile, characteristics of the apatite crystals formed by the system, release mechanisms, toxicity, mucoadhesion properties, system permanence time, adhesion strength of the liquid-crystalline system and color change of the remineralizated enamel, in addition to the assessment of the physicochemical properties of the remineralizated tissue. The results obtained in each of the different analyzes, according to the data distribution, will be subjected to appropriate statistical tests, with a significance level of 5%. (AU)