Effects of Photobiomodulation therapy in the wound healing: in vitro and in vivo assay

• Grant number: 22/13470-4
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2024
• End date: February 28, 2026
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal Investigator: Giovana Tofoli Moniz
• Grantee: Giovana Tofoli Moniz
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil
• Associated researchers: Aguinaldo Silva Garcez Segundo

Abstract

Photobiomodulation therapy is emerging as an effective strategy for the management of wound healing. The healing process of cutaneous lesions is considered a complex event that involves coagulation, inflamattions, fibroplasty and collagen deposition, and responds satisfactorily to photobiomodulation that can assist tissue repair. However, there are no consensus regarding the type of photobiomodulation and how it can involve the NFºB and Nrf2 in wound healing. Thus, the aim of this study is to evaluate the effect photobiomodulation in the cutaneous wound healing process. The treatments will be first evaluated in vitro with MTT scratch assays. After the in vitro evaluation, the best conditions for photobiomodulation will be used in vivo. Laser will be applied to Wistar rats in one of the two excisional wounds on the back of the animals, one cranial and the other caudal. The animals (n=12) will be divided in four groups: control group (without treatment, in the caudal lesion of the same animal); red; green, blue and violet light groups. Samples were collected on the 3th, 7th and 14th days for histological analysis of HE and immunohistochemistry (Collagen1 e VEGF). Also, ELISA kits will be used to determine the levels of the cytokines TNF-alfa, IL-1 beta, IL-6 and IL-10. Gene expression of NF kappa beta, Nrf2 e TGF-beta will be evaluated by real-time RT-qPCR. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAPINI, JULIANA ZAMPOLI BOAVA; ESTEVES, BRUNO DE ASSIS; OLIVEIRA, VAGNER GOMES DE SOUZA; ABDALLA, HENRIQUE BALLASSANI; CEREDA, CINTIA MARIA SAIA; DE ARAUJO, DANIELE RIBEIRO; TOFOLI, GIOVANA RADOMILLE. Analgesic Effect of Sulforaphane: A New Application for Poloxamer-Hyaluronic Acid Hydrogels. GELS, v. 10, n. 7, p. 14-pg., 2024-07-01. (22/13470-4, 19/06969-0)