Phage display and immune system: identification of molecular targets with diagnostic and therapeutic properties in immunological disorders

• Grant number: 04/02721-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2005
• End date: October 31, 2008
• Field of knowledge: Biological Sciences - Immunology
• Principal Investigator: Jorge Elias Kalil Filho
• Grantee: Jorge Elias Kalil Filho
• Host Institution: Fundação Zerbini. São Paulo , SP, Brazil
• Pesquisadores principais: Luisa Lina Villa
• Associated research grant(s): 06/54249-6 - Identification of molecular markers of atherosclerotic vessels., AR.EXT

Abstract

Peptide phage display is a powerful tool that utilizes bacteriophages to screen cells, tissues or organs in search of receptor-pair ligands. Phage display particles are like biological nanoprobes, capable of interacting with the target though small peptides, genetically engineered to be expressed on the virus outer coat. Since the peptide sequence information is coded by bacteriophage genome, a single virus particle bound to a cell or tissue sample can be recovered by bacterial infection, amplified and the peptide identified. Few techniques allow studying molecular interactions of single molecules or cell without previous knowledge of the target. The aim of this project is to establish a web of knowledge and think tank in phage display technology in Brazil. The technique will be employed at full capacity, in different approaches but the main theme will be the immune system. Specifically: identification of epitopes in humoral response (lgG) to Papilomavirus and acute renal vascular rejection (Component A); identification of auto immune CD4+ T cell epitopes involved in the rheumatic fever disease (Component B); inflammation and endothelial cells in the atherosclerotic disease (Component C); B1 Iymphocytes and tumor growth (Component O); thymus and regulatory cells development (Component E); study of the structural behavior of selected peptides and the dynamic of interaction with their respective receptors by nuclear magnetic resonance. Below are the experimental details of each component of the project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, ROSEMEIRE A.; GIORDANO, RICARDO J.; GUTIERREZ, PAULO S.; ROCHA, VIVIANE Z.; RUDNICKI, MARTINA; KEE, PATRICK; ABDALLA, DULCINEIA S. P.; PUECH-LEAO, PEDRO; CARAMELLI, BRUNO; ARAP, WADIH; et al. CTHRSSVVC Peptide as a Possible Early Molecular Imaging Target for Atherosclerosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 17, n. 9, SEP 2016. (04/02721-8)

SILVA, ROSEMEIRE A.; GIORDANO, RICARDO J.; GUTIERREZ, PAULO S.; ROCHA, VIVIANE Z.; RUDNICKI, MARTINA; KEE, PATRICK; ABDALLA, DULCINEIA S. P.; PUECH-LEAO, PEDRO; CARAMELLI, BRUNO; ARAP, WADIH; et al. CTHRSSVVC Peptide as a Possible Early Molecular Imaging Target for Atherosclerosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 17, n. 9, p. 18-pg., 2016-09-01. (04/02721-8)