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Heterotypic signaling between epithelial tumor cells and fibroblasts in carcinoma of the breast

Grant number: 04/04607-8
Support type:Research Projects - Thematic Grants
Duration: May 01, 2005 - July 31, 2008
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Maria Mitzi Brentani
Grantee:Maria Mitzi Brentani
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The predominant stromal cell type in breast carcinomas is the fibroblast. These fibroblasts are phenotypically different from their normal counterparts and they present several altered features such as proliferative potential, expression of growth factors and production of different ECM proteins. Moreover tumor environmental is involved in angiogenesis and invasion. Thus, fibroblasts may be a critical impact on various therapeutic strategies and also should be considered as potential targets of anti tumor treatment. However few genes involved in this interaction have been described. Our main goal in the present work is analyzing the molecular mediators of the heterotypic signaling interactions between epithelial cells and fibroblasts. Specifically, human breast tumor tissues are available from breast cancer surgery, enabling the establishment of fibroblasts primary cultures. Otherwise, fibroblasts can be obtained by laser dissection. Our first step is to quantify individual gene expression in RNA extracted from fibroblasts of tumors obtained from patients at different clinical stages by serial analysis of gene expression (SAGE) and compare the gene pattern obtained with that measured in RNAS of fibroblasts obtained from mammoplasties. Once candidate genes have been identified, their differential expression will be validated by real time PCR. Chromosomal imbalance will be determined by comparative genomic hybridization (CGH). (AU)

Articles published in Pesquisa FAPESP Magazine about the research grant:
Unexpected supporting actors 
Unexpected supporting actors 

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROZENCHAN, PATRICIA BORTMAN; PASINI, FATIMA SOLANGE; ROELA, ROSIMEIRE A.; HIRATA KATAYAMA, MARIA LUCIA; LOPES MUNDIM, FIORITA GONZALES; BRENTANI, HELENA; LYRA, EDUARDO C.; BRENTANI, MARIA MITZI. Specific upregulation of RHOA and RAC1 in cancer-associated fibroblasts found at primary tumor and lymph node metastatic sites in breast cancer. TUMOR BIOLOGY, v. 36, n. 12, p. 9589-9597, DEC 2015. Web of Science Citations: 11.
LOGULLO, ANGELA FLAVIA; AGATA STIEPCICH, MONICA MARIA; BUENO DE TOLEDO OSORIO, CINTIA APARECIDA; NONOGAKI, SUELI; PASINI, FATIMA SOLANGE; ROCHA, RAFAEL MALAGOLI; SOARES, FERNANDO AUGUSTO; BRENTANI, MARIA M. Role of Fos-related antigen 1 in the progression and prognosis of ductal breast carcinoma. Histopathology, v. 58, n. 4, p. 617-625, MAR 2011. Web of Science Citations: 12.
LOGULLO, ANGELA FLAVIA; NONOGAKI, SUELY; PASINI, FATIMA SOLANGE; BUENO DE TOLEDO OSORIO, CYNTHIA APARECIDA; SOARES, FERNANDO AUGUSTO; BRENTANI, M. MITZI. Concomitant expression of epithelial-mesenchymal transition biomarkers in breast ductal carcinoma: Association with progression. ONCOLOGY REPORTS, v. 23, n. 2, p. 313-320, FEB 2010. Web of Science Citations: 45.
ROZENCHAN, PATRICIA BORTMAN; CARRARO, DIRCE MARIA; BRENTANI, HELENA; DE CARVALHO MOTA, LOUISE DANIELLE; BASTOS, ELEN PEREIRA; NAPOLITANO E FERREIRA, ELISA; TORRE, CESAR H.; HIRATA KATAYAMA, MARIA LUCIA; ROELA, ROSIMEIRE APARECIDA; LYRA, EDUARDO C.; SOARES, FERNANDO AUGUSTO; AZEVEDO KOIKE FOLGUEIRA, MARIA APARECIDA; GUEDES SAMPAIO GOES, JOAO CARLOS; BRENTANI, MARIA MITZI. Reciprocal changes in gene expression profiles of cocultured breast epithelial cells and primary fibroblasts. International Journal of Cancer, v. 125, n. 12, p. 2767-2777, DEC 15 2009. Web of Science Citations: 41.
A.C. MARCHIORI; D.A. CASOLARI; M.A. NAGAI. Transcriptional up-regulation of PHLDA1 by 17β-estradiol in MCF-7 breast cancer cells. Brazilian Journal of Medical and Biological Research, v. 41, n. 7, p. -, Jul. 2008.
MARCHIORI, A. C.; CASOLARI, D. A.; NAGAI, M. A. Transcriptional up-regulation of PHLDA1 by 17 beta-estradiol in MCF-7 breast cancer cells. Brazilian Journal of Medical and Biological Research, v. 41, n. 7, p. 579-582, JUL 2008. Web of Science Citations: 10.

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