Analysis of exported vesicular structures and of secreted molecules by the pathogenic fungi Paracoccidioides brasiliensis e Cryptococcus neoformans

• Grant number: 06/05095-6
• Support Opportunities: Research Projects - Thematic Grants
• Start date: November 01, 2007
• End date: July 31, 2013
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Rosana Puccia
• Grantee: Rosana Puccia
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Adriana Karaoglanovic Carmona ; Carlos Pelleschi Taborda ; Edna Freymüller Haapalainen ; Leonardo Nimrichter ; Marcio Lourenço Rodrigues ; Zoilo Pires de Camargo
• Associated scholarship(s): 10/03193-6 - Heterolougous expression and antigenic characterization of Plgp43 from Paracoccidioides lutzii, BP.MS; 09/06618-0 - Cellular localization of MDJ1 chaperone from Paracoccidioides brasiliensis: comparison between isolates and related fungal species under stress growth conditions, BP.MS; 07/04757-8 - Structural and functional characterization of vesicular structures exported by the pathogenic fungus Paracoccidioides brasiliensis, BP.DR

Abstract

We aim at using the fungal pathogens Paracoccidioides brasiliensis and Cryptococcus neoformans as model systems to the study of membranous vesicular structures (exosome-like) and of cell wall components. We intend to isolate and characterize the vesicles, to determine their composition in comparison with cell wall fractions, to evaluate their immunomodulatory potential and the role of monohexosyl ceramide in their secretion mechanisms. In C. neoformans, we intend to understand the role of these vescicles in secretion and distal growth of the capsular material. The importance of extracellular vesicles as a secretory mechanism, as a means of transportation for immunomodulatory and/or relevant components in the host/parasite relationship has been recently recognized. In fungi, however, their presence has only been detected by the groups composing this project and reported in congress abstracts. The analyses will be held in two isolates of P. brasiliensis that evoke mice infections with distinct profiles. Other secreted P. brasiliensis molecules will also be addressed here: chaperone Mdj1 recently found in the cell wall, recombinant isoforms of the gp43 antigen, whose patent has been asked, and a recombinant exocelular subtilase-like proteinase to be hopefully expressed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEITAO, JR., NATANAEL P.; VALLEJO, MILENE C.; CONCEICAO, PALLOMA M.; CAMARGO, ZOILO P.; HAHN, ROSANE; PUCCIA, ROSANA. Paracoccidioides lutzii Plp43 Is an Active Glucanase with Partial Antigenic Identity with P. brasiliensis gp43. PLoS Neglected Tropical Diseases, v. 8, n. 8, AUG 2014. (06/05095-6, 10/03193-6)

VALLEJO, MILENE C.; NAKAYASU, ERNESTO S.; LONGO, LARISSA V. G.; GANIKO, LUCIANE; LOPES, FELIPE G.; MATSUO, ALISSON L.; ALMEIDA, IGOR C.; PUCCIA, ROSANA. Lipidomic Analysis of Extracellular Vesicles from the Pathogenic Phase of Paracoccidioides brasiliensis. PLoS One, v. 7, n. 6, p. e39463, 2012. (07/04757-8, 06/05095-6)

GUILHEN LONGO, LARISSA VALLE; BREYER, CARLOS ALEXANDRE; NOVAES, GABRIELA MACHADO; GEGEMBAUER, GREGORY; LEITAO JR, NATANAEL PINHEIRO; OCTAVIANO, CARLA ELIZABETE; TOYAMA, MARCOS HIKARI; DE OLIVEIRA, MARCOS ANTONIO; PUCCIA, ROSANA. The Human PathogenParacoccidioides brasiliensisHas a Unique 1-Cys Peroxiredoxin That Localizes Both Intracellularly and at the Cell Surface. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, AUG 4 2020. (13/07937-8, 17/19942-7, 06/05095-6, 13/25950-1, 17/20291-0, 11/13500-6)