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Association between polymorphism of the eosinophil cationic protein-gene and tumor-associated tissue eosinophilia in oral squamous cell carcinomas

Grant number: 06/03830-0
Support type:Regular Research Grants
Duration: January 01, 2007 - December 31, 2008
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal researcher:Denise Tostes Oliveira
Grantee:Denise Tostes Oliveira
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Eosinophil cationic protein (ECP), located in the specific granules of eosinophils, presents many biological activities but its cytotoxic capacity to kill tumoral cells seems to be of particular interest. The functional role of eosinophils in solid malignant tumors, such as squamous cell carcinoma (SCC), remains uncertain. The aim of this study is to investigate the prevalence of the ECP gene polymorphism 434(G>C) in patients with oral SCC and its possible correlation with tumor-associated tissue eosinophilia (TATE) and patients' outcome. From 1994 to 1998, patients newly diagnosed and histologically confirmed SCC of the oral cavity, who were admitted for surgical treatment at the Head and Neck Surgery and Otorhinolaryngology Department of the Hospital A.C. Camargo, Brazil, will be enrolled to this study. TATE will be determined from surgically resected specimens obtained from the Department of Pathology files of the Hospital A.C. Camargo. Polymorphism analysis will be performed at NeoGene Laboratory at Urology Department of Universidade Estadual Paulista (UNESP), using genomic DNA extracted from peripheral blood. The genotype 434 will be detected by the cleavage of a DNA specific sequence amplified by the PstI restriction enzyme and the cleaved products will be subsequently analyzed on an agarose gel. The associations between clinical variables with polymorphism and TATE will be determined by chi-square or Fisher exact test. Disease-free survival and overall survival will be calculated by the Kaplan-Meier product-limit actuarial method and the comparison of the survival curves will be performed using log rank test. Statistical differences between groups will be assumed for p values <0.05. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, MICHELE CONCEICAO; OLIVEIRA, DENISE TOSTES; KOWALSKI, LUIZ PAULO. The role of eosinophils and eosinophil cationic protein in oral cancer: A review. ARCHIVES OF ORAL BIOLOGY, v. 56, n. 4, p. 353-358, APR 2011. Web of Science Citations: 16.
PEREIRA, MICHELE C.; OLIVEIRA, DENISE T.; OLIVIERI, ELOISA H. R.; ROGATTO, SILVIA R.; CARVALHO, ANDRE L.; LANDMAN, GILLES; KOWALSKI, LUIZ P. The 434(G > C) polymorphism in the eosinophil cationic protein gene and its association with tissue eosinophilia in oral squamous cell carcinomas. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 39, n. 1, p. 56-62, JAN 2010. Web of Science Citations: 4.

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