Cellular and molecular biology of the kallikrein-kinin and renin-angiotensin systems

• Grant number: 08/06676-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2009
• End date: May 31, 2013
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: João Bosco Pesquero
• Grantee: João Bosco Pesquero
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Adriana Karaoglanovic Carmona ; Clélia Rejane Antõnio ; Clovis Ryuichi Nakaie ; Dulce Elena Casarini ; Edson Lucas dos Santos ; Jorge Luiz Pesquero ; Kely de Picoli Souza
• Associated scholarship(s): 12/10743-8 - Relationship between Muscle Injury in Brazilian Elite Athletes and Polymorphisms of Angiotensin I Converting Enzyme, Bradykinin Receptor B2 and CK-MM, BP.PD; 11/18609-6 - PARTICIPATION OF THE B1 AND B2 KININ RECEPTORS IN FIBRODYSPLASIA OSSIFICANS PROGRESSIVA (FOP, BP.DR; 11/12076-6 - Cellular and molecular biology of the kallikrein-kinin and renin-angiotensin systems, BP.IC; 10/01819-5 - Effect of the kinin receptors deficiency in the regulation of blood pressure and the renin-angiotensin system, BP.PD; 10/00711-6 - THE ROLE OF KALLIKREIN-KININ SYSTEM IN THE DEVELOPMENT OF FIBRODYSPLASIA OSSIFICANS PROGRESSIVA: GENERATION OF ANIMAL MODEL, BP.PD

Abstract

The Renin-Angiotensin (RAS) and Kalikrein-Kinin (KKS) systems play an important role in the regulation of several physiological and pathologic processes related to the control of blood pressure and also affect a large number of processes such as cell growth and proliferation, pain and inflammation, among others. The main effector substance of the RAS is angiotensin II (Ang II), and those of the KKS are bradykinin (BK) and other kinins. Kinins, as well as Ang II act through the activation of G protein coupled receptors (GPCRs): angiotensin receptors AT1 and AT2, and BK receptors B1 e B2 Due to their importance in physiopathological processes involving the regulation of the blood pressure, and because of the great homology between their receptors, the RAS and the KKS have been the object of study of our laboratories, involving molecular modeling and site-directed mutagenesis of the AT1 receptor, conformational studies of synthetic peptides related to the AT1 receptor and their interaction with membranes, and various aspects of the molecular biology of the two systems. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES-ZILLO, THAIS OLIVEIRA; PUGLIESE, LIVIA SOUZA; TOLEDO SALES, VICENCIA MICHELINE; DA SILVA MORI, MARCELO ALVES; SQUAIELLA-BAPTISTAO, CARLA CRISTINA; LONGO-MAUGERI, IEDA MARIA; LOPES, JOSE DANIEL; DE OLIVEIRA, SUZANA MACEDO; MONTEIRO, ANA CAROLINA; PESQUERO, JOAO BOSCO. Increased bone loss and amount of osteoclasts in kinin B1 receptor knockout mice. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 40, n. 7, p. 653-660, JUL 2013. (08/06676-8)

LOIOLA, RODRIGO A.; REIS, FELIPE C. G.; KAWAMOTO, ELISA M.; SCAVONE, CRISTOFORO; ABDALLA, DULCINEIA S.; FERNANDES, LILIAM; PESQUERO, JOAO BOSCO. Role of vascular Kinin B-1 and B-2 receptors in endothelial nitric oxide metabolism. Peptides, v. 32, n. 8, p. 1700-1705, AUG 2011. (08/06676-8)

GUETHE, L. M.; PELEGRINI-DA-SILVA, A.; BORELLI, K. G.; JULIANO, M. A.; PELOSI, G. G.; PESQUERO, J. B.; SILVA, C. L. M.; CORREA, F. M. A.; MURAD, F.; PRADO, W. A.; et al. ANGIOTENSIN (5-8) MODULATES NOCICEPTION AT THE RAT PERIAQUEDUCTAL GRAY VIA THE NO-sGC PATHWAY AND AN ENDOGENOUS OPIOID. Neuroscience, v. 231, p. 315-327, FEB 12 2013. (08/06676-8)

BATISTA, ELICE CARNEIRO; CARVALHO, LUIZ ROBERTO; CASARINI, DULCE ELENA; CARMONA, ADRIANA KARAOGLANOVIC; DOS SANTOS, EDSON LUCAS; DA SILVA, ELTON DIAS; DOS SANTOS, ROBSON AUGUSTO; NAKAIE, CLOVIS RYUICHI; MUNOZ ROJAS, MARIA VERONICA; DE OLIVEIRA, SUZANA MACEDO; et al. ACE activity is modulated by the enzyme alpha-galactosidase A. JOURNAL OF MOLECULAR MEDICINE-JMM, v. 89, n. 1, p. 65-74, JAN 2011. (08/06676-8)

TURACA, LAURO THIAGO; PESSOA, JULIANA GILBERT; MOTTA, FABIANA LOUISE; MUNOZ ROJAS, MARIA VERONICA; MUELLER, KAREN BARBOSA; LOURENCO, CHARLES MARQUES; MARQUES, WILSON JUNIOR; D'ALMEIDA, VANIA; MARTINS, ANA MARIA; PESQUERO, JOAO BOSCO. New mutations in the GLA gene in Brazilian families with Fabry disease. JOURNAL OF HUMAN GENETICS, v. 57, n. 6, p. 347-351, JUN 2012. (08/06676-8)

REIS, FELIPE C. G.; HARO, ANDERSON S.; BACURAU, ALINE V. N.; HIRABARA, SANDRO M.; WASINSKI, FREDERICK; ORMANJI, MILENE S.; MOREIRA, JOSE B. N.; KIYOMOTO, BEATRIZ H.; BERTONCINI, CLELIA R. A.; BRUM, PATRICIA C.; et al. Deletion of Kinin B2 Receptor Alters Muscle Metabolism and Exercise Performance. PLoS One, v. 10, n. 8, AUG 24 2015. (13/04757-9, 08/06676-8)

MERINO, VANESSA F.; TODIRAS, MIHAIL; MORI, MARCELO A.; SALES, VICENCIA M. T.; FONSECA, RAPHAEL G.; SAUL, VERA; TENNER, KATJA; BADER, MICHAEL; PESQUERO, JOAO B.. Predisposition to atherosclerosis and aortic aneurysms in mice deficient in kinin B1 receptor and apolipoprotein E. JOURNAL OF MOLECULAR MEDICINE-JMM, v. 87, n. 10, p. 953-963, OCT 2009. (08/06676-8)

LOIOLA, RODRIGO A.; TORRES, TATHIANY C.; LANDGRAF, RICHARDT G.; PESQUERO, JOAO BOSCO; FERNANDES, LILIAM. Cellular Changes Induced by Kinin B-1 Receptor Deletion: Study of Endothelial Nitric Oxide Metabolism. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, v. 21, n. 3, p. 375-382, SEP 2015. (14/18760-4, 08/06676-8, 10/01404-0)

FONSECA, RAPHAEL GOMES; SALES, VICENCIA MICHELINE; ROPELLE, EDUARDO; BARROS, CARLOS CASTILHO; OYAMA, LILA; IUKI IHARA, SILVIA SAIULI; ABDALLA SAAD, MARIO JOSE; ARAUJO, RONALDO CARVALHO; PESQUERO, JOAO BOSCO. Lack of kinin B-1 receptor potentiates leptin action in the liver. JOURNAL OF MOLECULAR MEDICINE-JMM, v. 91, n. 7, p. 851-860, JUL 2013. (08/06676-8)

LOIOLA, RODRIGO A.; TORRES, TATHIANY C.; ABURAYA, CARLA M.; LANDGRAF, MARISTELLA A.; LANDGRAF, RICHARDT G.; PESQUERO, JOAO BOSCO; FERNANDES, LILIAM. Generation and characterization of a spontaneously immortalized endothelial cell line from mice microcirculation. Experimental Cell Research, v. 319, n. 8, p. 1102-1110, MAY 1 2013. (08/06676-8, 10/01404-0)

TURACA, LAURO THIAGO; SOARES DE FARIA, DOUGLAS OLIVEIRA; KYOSEN, SANDRA OBIKAWA; TEIXEIRA, VALBER DIAS; MOTTA, FABIANA LOUISE; PESSOA, JULIANA GILBERT; RODRIGUES E SILVA, MARINA; DE ALMEIDA, SANDRO SOARES; D'ALMEIDA, VANIA; MUNOZ ROJAS, MARIA VERONICA; et al. Novel GAA mutations in patients with Pompe disease. Gene, v. 561, n. 1, p. 124-131, APR 25 2015. (08/06676-8)